Rate of transcription elongation and sequence-specific pausing by RNA polymerase I directly influence rRNA processing

Autor: Abigail K. Huffines, Krysta L. Engel, Sarah L. French, Yinfeng Zhang, Olga V. Viktorovskaya, David A. Schneider

Publikováno v: The Journal of biological chemistry. 298(12)

One of the first steps in ribosome biogenesis is transcription of the ribosomal DNA by RNA polymerase I (Pol I). Processing of the resultant rRNA begins cotranscriptionally, and perturbation of Pol I transcription elongation results in defective rRNA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d99b1a9112fcfcbb007b9fa3f4fd40c1
https://pubmed.ncbi.nlm.nih.gov/36423683

Kinetic analysis demonstrates a requirement for the Rat1 exonuclease in cotranscriptional pre-rRNA cleavage.

Autor: Konstantin Axt, Sarah L French, Ann L Beyer, David Tollervey

Publikováno v: PLoS ONE, Vol 9, Iss 2, p e85703 (2014)

During yeast ribosome synthesis, three early cleavages generate the 20S precursor to the 18S rRNA component of the 40S subunits. These cleavages can occur either on the nascent transcript (nascent transcript cleavage; NTC) or on the 35S pre-rRNA that

Externí odkaz: https://doaj.org/article/3462cf860d8f4bd5a6029673144eb2c0

Nascent Transcript Folding Plays a Major Role in Determining RNA Polymerase Elongation Rates

Autor: Benjamin D. Goddard, Aleksandra Helwak, Elisabeth Petfalski, Tomasz W. Turowski, David Tollervey, Sarah L. French

Publikováno v: Molecular Cell
Turowski, T, Petfalski, E, Goddard, B D, French, S L, Helwak, A & Tollervey, D 2020, ' Nascent transcript folding plays a major role in determining RNA polymerase elongation rates ', Molecular Cell, vol. 79, no. 3, pp. 488-503.e11 . https://doi.org/10.1016/j.molcel.2020.06.002

Summary Transcription elongation rates influence RNA processing, but sequence-specific regulation is poorly understood. We addressed this in vivo, analyzing RNAPI in S. cerevisiae. Mapping RNAPI by Miller chromatin spreads or UV crosslinking revealed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a4ebe0de430ec8a2e5befdc70a6e39e
https://doi.org/10.1016/j.molcel.2020.06.002

Cohesion promotes nucleolar structure and function

Autor: Brian D. Slaughter, Rhonda Trimble Ross, Ann L. Beyer, Bethany Harris, Tania Bose, Sarah L. French, Jay R. Unruh, Fei Wang, Ying Zhang, Jennifer L. Gerton, Shuai Lu, Kenneth K. Lee

Publikováno v: Molecular Biology of the Cell

Mutations in the cohesin acetyltransferase Eco1 or the cohesin ring compromise nucleolar function in budding yeast. A mutation in Eco1 that is associated with the human disease Roberts syndrome compromises looping interactions at the ribosomal DNA an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8456d76756880756b8ee2a0aea42fdaa
https://doi.org/10.1091/mbc.e13-07-0377

Distinguishing the Roles of Topoisomerases I and II in Relief of Transcription-Induced Torsional Stress in Yeast rRNA Genes

Autor: Robert D. Hontz, Aziz El Hage, David Tollervey, Jeffrey S. Smith, M. Mitchell Smith, Tashima E. Lambert, Ann L. Beyer, Sarah L. French, Martha L. Sikes, Yvonne N. Osheim

Publikováno v: French, S L, Sikes, M L, Hontz, R D, Osheim, Y N, Lambert, T E, El Hage, A, Smith, M M, Tollervey, D, Smith, J S & Beyer, A L 2011, ' Distinguishing the Roles of Topoisomerases I and II in Relief of Transcription-Induced Torsional Stress in Yeast rRNA Genes ', Molecular and Cellular Biology, vol. 31, no. 3, pp. 482-494 . https://doi.org/10.1128/MCB.00589-10

To better understand the role of topoisomerase activity in relieving transcription-induced supercoiling, yeast genes encoding rRNA were visualized in cells deficient for either or both of the two major topoisomerases. In the absence of both topoisome

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b4e1371892ccafbdddf262b184008dc
https://doi.org/10.1128/mcb.00589-10