Macrophage migration inhibitory factor is required for NLRP3 inflammasome activation

Autor: Tali Lang, Jacinta P. W. Lee, Kirstin Elgass, Anita A. Pinar, Michelle D. Tate, Elizabeth H. Aitken, Huapeng Fan, Sarah J. Creed, Nadia S. Deen, Daouda A. K. Traore, Ivo Mueller, Danielle Stanisic, Francesca S. Baiwog, Colin Skene, Matthew C. J. Wilce, Ashley Mansell, Eric F. Morand, James Harris

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-15 (2018)

MIF is a cytokine best known for its modulatory effect on expression of proinflammatory cytokines. Here the authors show that MIF facilitates the NLRP3–vimentin interaction, resulting in Nlrp3 inflammasome activation.

Externí odkaz: https://doaj.org/article/e3f4f9c8a1b94be9979682bf263d2d83

An Optimized and Detailed Step-by-Step Protocol for the Analysis of Neuronal Morphology in Golgi-Stained Fetal Sheep Brain

Autor: Ingrid Dudink, Tegan A. White, Maryam Ardalan, Carina Mallard, Giulia Ballerin, Sarah J. Creed, Yen Pham, Amy E. Sutherland, Margie Castillo-Melendez, Beth J. Allison, Suzanne L. Miller

Publikováno v: Dudink, I, A. White, T, Ardalan, M, Mallard, C, Ballerin, G, J. Creed, S, Pham, Y, E Sutherland, A, Castillo-Melendez, M, Allison, B & L Miller, S 2022, ' An optimized and detailed step-by-step protocol for the analysis of neuronal morphology in golgi-stained fetal sheep brain ', Developmental Neuroscience .

Antenatal brain development during the final trimester of human pregnancy is a time when mature neurons become increasingly complex in morphology, through axonal and dendritic outgrowth, dendritic branching, and synaptogenesis, together with myelin p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51fc7fdf2f5efa86c81649d50c2cf8e9
https://pubmed.ncbi.nlm.nih.gov/35447627

Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis

Autor: Stephen R. Holdsworth, Sarah J. Creed, Kim M. O’Sullivan, Poh-Yi Gan

Publikováno v: Journal of visualized experiments : JoVE. (160)

Glomerular cell death is a pathological feature of myeloperoxidase anti neutrophil cytoplasmic antibody associated vasculitis (MPO-AAV). Extracellular deoxyribonucleic acid (ecDNA) is released during different forms of cell death including apoptosis,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ff874dc965e8ee3a63350cfbdade601
https://pubmed.ncbi.nlm.nih.gov/32628162

Microscopy Methods for Imaging MIF and Its Interaction Partners

Autor: Kirstin D, Elgass, Sarah J, Creed, Ina, Rudloff

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2080

Fluorescence microscopy has become a powerful tool to investigate proteins in their natural environment. Well-established techniques like widefield and confocal fluorescence microscopy have commonly been used for decades to visualize biomolecules in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::4db07fdcef23025014e8a011c6c263f8
https://pubmed.ncbi.nlm.nih.gov/31745874

Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM)

Autor: Matthew McKenzie, Sarah J. Creed

Publikováno v: Methods in Molecular Biology ISBN: 9781493990269

The mitochondrial membrane potential (Δψm) drives the generation of ATP by mitochondria. Interestingly, Δψm is higher in many cancer cells comparted to healthy noncancerous cell types, providing a unique metabolic marker. This feature has also be

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ddd382b935b75ce82b9c37f64c4f85e3
https://doi.org/10.1007/978-1-4939-9027-6_5