Role of metalloproteases in the CD95 signaling pathways

Autor: Laurent Devel, Nicolas Guedeney, Sarah Bregant, Animesh Chowdhury, Mickael Jean, Patrick Legembre

Publikováno v: Frontiers in Immunology, Vol 13 (2022)

CD95L (also known as FasL or CD178) is a member of the tumor necrosis family (TNF) superfamily. Although this transmembrane ligand has been mainly considered as a potent apoptotic inducer in CD95 (Fas)-expressing cells, more recent studies pointed ou

Externí odkaz: https://doaj.org/article/dd73535bfe1845b39f323c06cc290558

Ligand‐Directed Modification of Active Matrix Metalloproteases: Activity‐based Probes with no Photolabile Group

Autor: Marion Doladilhe, Vincent Dive, Olivier Lequin, Pierrick Bruyat, Monika Kaminska, Dimitris Georgiadis, Carole Fruchart Gaillard, Mélissa De Souza, Robert Thai, Evelyne Cassar-Lajeunesse, Carole Malgorn, Laurent Devel, Andrzej Galat, Fabrice Beau, Sarah Bregant, Isabelle Correia

Publikováno v: Angewandte Chemie International Edition
Angewandte Chemie International Edition, 2021, 60 (33), pp.18272-18279. ⟨10.1002/anie.202106117⟩

International audience; Activity-based probes enable discrimination between the active enzyme and its inactive or inactivated counterparts. Since metalloproteases catalysis is non-covalent, activity-based probes targeting them have been systematicall

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71534c08742b02912c580f9fcb997039
https://doi.org/10.1002/ange.202106117

Sydnone-based turn-on fluorogenic probes for no-wash protein labeling and in-cell imaging

Autor: Antoine Sallustrau, Manas R. Pattanayak, Marc Nothisen, Sarah Bregant, Alain Wagner, Lucie Plougastel, Frédéric Taran, Davide Audisio, Margaux Riomet

Publikováno v: Chemical Communications. 55:4582-4585

We report the synthesis and use of sydnone-based profluorophores as tools for imaging applications. These new probes display exquisite reactivity towards strain promoted cycloaddition reactions with cycloalkynes allowing fast, efficient and selective

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b515f89aff1cb26c7ebada444ee029b6
https://doi.org/10.1039/c9cc01458f

DMF-MALDI: droplet based microfluidic combined to MALDI-TOF for focused peptide detection

Autor: Robert Thai, Sarah Bregant, Kiarach Mesbah, Florent Malloggi

Publikováno v: Scientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
Scientific Reports
Scientific Reports, Nature Publishing Group, 2017, 7 (1), pp.6756. ⟨10.1038/s41598-017-06660-8⟩
Scientific Reports, 2017, 7 (1), pp.6756. ⟨10.1038/s41598-017-06660-8⟩

We present an automated droplet microfluidic system (DMF) to generate monitored nanoliter aqueous droplets in oil and their deposition on a commercial stainless steel plate for MALDI-TOF analysis of peptides or protein digests. We demonstrate that DM

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::665a1a2c1974fe036403a47a4a275af8
https://doaj.org/article/e69f693adbf54fc9b48ca747cf076df1

Detection of Endogenous Matrix Metalloprotease-12 Active Form with a Novel Broad Spectrum Activity-based Probe*

Autor: Sarah Bregant, Vincent Dive, Fannely Berthon, Bertrand Czarny, Evelyne Cassar-Lajeunesse, Catherine Nury

Publikováno v: Journal of Biological Chemistry. 288:5636-5644

Matrix metalloproteases (MMPs) have attracted considerable attention as critical mediators of pathological tissue remodeling processes. However it remains an unresolved challenge to detect their active forms in biological samples. To prove the effica

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46fe920a9a446118fd89ea0dcd0ecfa5
https://doi.org/10.1074/jbc.m112.419499