Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Sarah B. Batt"'
Combinatorial Enzyme Approach for Production and Screening of Libraries of Feruloyl Oligosaccharides
Publikováno v:
Advances in Enzyme Research. :27-37
Combinatorial chemistry involves the chemical or biological synthesis of diverse variation of the structures of a target molecule and the library is then screened for variants of desirable target properties. The approach has been a focus of research
Publikováno v:
Industrial Biotechnology. 6:112-118
Corn-ethanol production requires enzymatic hydrolysis of the starch prior to yeast fermentation in a two-stage process. To create yeast capable of producing both endo- and exo-acting starch-degrading enzymes, recombinant constructs of Hordeum vulgare
Publikováno v:
Protein & Peptide Letters. 10:459-468
High-throughput screening for high-activity barley alpha-amylase mutants expressed in Saccharomyces cerevisiae is hampered by the interference of reducing agents, particularly the glucose used in yeast growth media. The present investigation employed
Publikováno v:
Journal of Protein Chemistry. 20:619-623
Recombinant barley alpha-amylase 1 isozyme was constitutively secreted by Saccharomyces cerevisiae. The enzyme was purified to homogeneity by ultrafiltration and affinity chromatography. The protein had a correct N-terminal sequence of His-Gln-Val-Le
Publikováno v:
Journal of Protein Chemistry. 19:373-377
Barley alpha-amylase was purified by ammonium sulfate fraction, ion-exchange, ultrafiltration, and gel filtration to homogeneity. The purified enzyme was partially digested with trypsin, and the reaction mixture was applied to a cyclohepta-amylose ep
Publikováno v:
Journal of industrial microbiologybiotechnology. 38(12)
The Aspergillus niger feruloyl esterase gene (faeA) was cloned into Saccharomyces cerevisiae via a yeast expression vector, resulting in efficient expression and secretion of the enzyme in the medium with a yield of ~2 mg/l. The recombinant enzyme wa
Publikováno v:
Protein and peptide letters. 17(6)
A novel exo-glucanase gene (xeg5B) was isolated from a rumenal microbial metagenome, cloned, and expressed in E. coli. The 1548 bp gene coded for a protein of 516 amino acids, which assumed an (a/b)(8) fold typical of glycoside hydrolase (GH) family
Publikováno v:
Applied microbiology and biotechnology. 79(6)
A novel exo-alpha-1,5-L-arabinanase gene (arn3) was isolated, cloned, and expressed in E. coli. The recombinant enzyme (ARN3) had a pH optimum of 6.0-7.0 and a pH 3.0-7.0 stability range. The temperature optimum was 50 degrees C with a stability less
Publikováno v:
The protein journal. 24(7-8)
The gene encoding Lentinula edodes glucoamylase (GLA) was cloned into Saccharomyces cerevisiae, expressed constitutively and secreted in an active form. The enzyme was purified to homogeneity by (NH4)2SO4 fractionation, anion exchange and affinity ch
Publikováno v:
Protein and peptide letters. 10(5)
High-throughput screening for high-activity barley alpha-amylase mutants expressed in Saccharomyces cerevisiae is hampered by the interference of reducing agents, particularly the glucose used in yeast growth media. The present investigation employed