Method for isolation of PCR-ready genomic DNA from zebrafish tissues

Autor: Nathan D. Meeker, Sarah A. Hutchinson, Linh Ho, Nikolaus S. Trede

Publikováno v: BioTechniques, Vol 43, Iss 5, Pp 610-614 (2007)

Here we describe a method for the isolation of PCR-ready genomic DNA from various zebrafish tissues that is based on a previously published murine protocol. The DNA solutions are of sufficient quality to allow PCR detection of transgenes from all com

Externí odkaz: https://doaj.org/article/392adcecec3e46bba9ee1dee247f9279

Feasibility of establishing a Canadian Obstetric Survey System (CanOSS) for severe maternal morbidity: a study protocol

Autor: Rohan D’Souza, Rebecca J Seymour, Marian Knight, Susie Dzakpasu, K S Joseph, Sara Thorne, Maria B Ospina, Jon Barrett, Jocelynn Cook, Deshayne B Fell, Heather Scott, Amy Metcalfe, Thomas van den Akker, Stephen Lapinsky, Leslie Skeith, Beth Murray-Davis, Prakesh Shah, Milena Forte, Rizwana Ashraf, Josie Chundamala, Sarah A Hutchinson, Kenneth K Chen, Isabelle Malhamé

Publikováno v: D'Souza, R, Seymour, R J, Knight, M, Dzakpasu, S, Joseph, K S, Thorne, S, Ospina, M B, Barrett, J, Cook, J, Fell, D B, Scott, H, Metcalfe, A, van den Akker, T, Lapinsky, S, Skeith, L, Murray-Davis, B, Shah, P, Forte, M, Ashraf, R, Chundamala, J, Hutchinson, S A, Chen, K K & Malhamé, I 2022, ' Feasibility of establishing a Canadian Obstetric Survey System (CanOSS) for severe maternal morbidity: a study protocol ', BMJ Open, vol. 12, no. 3, e061093, pp. 1-7 . https://doi.org/10.1136/bmjopen-2022-061093
BMJ Open, 12(3):e061093, 1-7. BMJ Publishing Group

IntroductionSevere maternal morbidity (SMM)—an unexpected pregnancy-associated maternal outcome resulting in severe illness, prolonged hospitalisation or long-term disability—is recognised by many, as the preferred indicator of the quality of mat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9b8f32b160b59b464dee8d65d40e90f
https://doi.org/10.1136/bmjopen-2022-061093

Synthesis and characterization of poly(N-isopropyl methacrylamide) core/shell nanogels for controlled release of chemotherapeutics

Autor: Nicholas A. Peppas, Jonathan T. Peters, Nisha Lizana, Sarah S. Hutchinson, Isha Verma

Publikováno v: Chemical Engineering Journal. 340:58-65

A method is presented for synthesizing and characterizing core/shell nanogels based around poly(N-isopropyl methacrylamide) (PNIPMAAm). The effectiveness of the sequential addition reaction scheme was determined for its impact on temperature response

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::236032450ef9e8c6866d7198876b9f33
https://doi.org/10.1016/j.cej.2018.01.009

Weevil borne microbes contribute as much to the reduction of photosynthesis in water hyacinth as does herbivory

Autor: Brad S. Ripley, Sarah-Leigh Hutchinson, Nic Venter, Martin Hill

Publikováno v: Biological Control. 64:138-142

Arthropods released for weed biocontrol can have effects other than simply removing biomass and frequently decrease photosynthetic rate more than can be attributed to the mere loss of photosynthetic surface area. Some of this effect may result becaus

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::541a57bdc0399b23e4b46a3c23674d9f
https://doi.org/10.1016/j.biocontrol.2012.10.011

Anti-CD8 antibodies can inhibit or enhance peptide-MHC class I (pMHCI) multimer binding: this is paralleled by their effects on CTL activation and occurs in the absence of an interaction between pMHCI and CD8 on the cell surface

Autor: Linda Wooldridge, P. Rod Dunbar, Vincenzo Cerundolo, David Price, Anna Lissina, Andrew K. Sewell, Emma Jones, Fareed Mirza, Sarah L. Hutchinson, Man-Lik Choi Edward

Publikováno v: Scopus-Elsevier
ResearcherID

Cytotoxic T lymphocytes recognize short peptides presented in association with MHC class I (MHCI) molecules on the surface of target cells. The Ag specificity of T lymphocytes is conferred by the TCR, but invariable regions of the peptide-MHCI (pMHCI

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1790155dee5b6f9ebb3aa23bfc34886
https://ora.ox.ac.uk/objects/uuid:b7d55420-e14b-4a04-b466-8182875bf4e0