Zobrazeno 1 - 10
of 37
pro vyhledávání: '"Sara Arana-Peña"'
Autor:
Sara Arana-Peña, Nathalia S. Rios, Diego Carballares, Carmen Mendez-Sanchez, Yuliya Lokha, Luciana R. B. Gonçalves, Roberto Fernandez-Lafuente
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
The lipase from Pseudomonas fluorescens (PFL) has been immobilized on octyl-agarose beads under 16 different conditions (varying pH, ionic strength, buffer, adding some additives) at two different loadings, 1 and 60 mg of enzyme/g of support with the
Externí odkaz:
https://doaj.org/article/eacc428aa9324285955c78f60617b5af
Autor:
Sabrina Ait Braham, El-Hocine Siar, Sara Arana-Peña, Diego Carballares, Roberto Morellon-Sterling, Hossein Bavandi, Diandra de Andrades, Jakub F. Kornecki, Roberto Fernandez-Lafuente
Publikováno v:
Molecules, Vol 26, Iss 4, p 968 (2021)
This paper aims to investigate the effects of some salts (NaCl, (NH4)2SO4 and Na2SO4) at pH 5.0, 7.0 and 9.0 on the stability of 13 different immobilized enzymes: five lipases, three proteases, two glycosidases, and one laccase, penicillin G acylase
Externí odkaz:
https://doaj.org/article/407a73dcf6fd46a8b4ecdf174f4a9e25
Publikováno v:
Catalysts, Vol 10, Iss 10, p 1207 (2020)
The lipases A and B from Candida antarctica (CALA and CALB), Thermomyces lanuginosus (TLL) or Rhizomucor miehei (RML), and the commercial and artificial phospholipase Lecitase ultra (LEU) may be co-immobilized on octyl agarose beads. However, LEU and
Externí odkaz:
https://doaj.org/article/60bfbced70d54e47a8ded29eebd1bddf
Autor:
Javier A. Martínez-Sanchez, Sara Arana-Peña, Diego Carballares, Malcom Yates, Cristina Otero, Roberto Fernandez-Lafuente
Publikováno v:
Catalysts, Vol 10, Iss 7, p 738 (2020)
Eversa® Transform (ET), and the lipase from Thermomyces lanuginosus (TLL), liquid commercial lipases formulations, have been immobilized on octyl agarose beads and their stabilities were compared. Immobilized and free ET forms were more thermostable
Externí odkaz:
https://doaj.org/article/adace0aa9f7e4bfb944508eb6f6adfc9
Autor:
Sara Arana-Peña, Diego Carballares, Ángel Berenguer-Murcia, Andrés R. Alcántara, Rafael C. Rodrigues, Roberto Fernandez-Lafuente
Publikováno v:
Catalysts, Vol 10, Iss 6, p 605 (2020)
Lipases are among the most utilized enzymes in biocatalysis. In many instances, the main reason for their use is their high specificity or selectivity. However, when full modification of a multifunctional and heterogeneous substrate is pursued, enzym
Externí odkaz:
https://doaj.org/article/8e9f7bc7dbd94cb0aea1bfe1f75bb965
Autor:
Nathalia S. Rios, Sara Arana-Peña, Carmen Mendez-Sanchez, Yuliya Lokha, Vicente Cortes-Corberan, Luciana R. B. Gonçalves, Roberto Fernandez-Lafuente
Publikováno v:
Catalysts, Vol 9, Iss 7, p 576 (2019)
A new strategy to increase the enzyme-loading capacity of porous supports was investigated. Lipase from Pseudomonas fluorescens (PFL) was immobilized on octyl-agarose (OA) beads and treated with polyethyleneimine (PEI). Then, PFL was immobilized on t
Externí odkaz:
https://doaj.org/article/836771c156dc460984dd54657f9cb72c
Autor:
Nathalia S. Rios, Sara Arana-Peña, Carmen Mendez-Sanchez, Claudia Ortiz, Luciana R. B. Gonçalves, Roberto Fernandez-Lafuente
Publikováno v:
Catalysts, Vol 9, Iss 5, p 487 (2019)
Coimmobilization of lipases may be interesting in many uses, but this means that the stability of the least stable enzyme determines the stability of the full combilipase. Here, we propose a strategy that permits the reuse the most stable enzyme. Lec
Externí odkaz:
https://doaj.org/article/1089dff32d5e4a7ab6227446244e8c0b
Autor:
Fouzia Hussain, Sara Arana-Peña, Roberto Morellon-Sterling, Oveimar Barbosa, Sabrina Ait Braham, Shagufta Kamal, Roberto Fernandez-Lafuente
Publikováno v:
Molecules, Vol 23, Iss 12, p 3188 (2018)
Alcalase was immobilized on glyoxyl 4% CL agarose beads. This permitted to have Alcalase preparations with 50% activity retention versus Boc-l-alanine 4-nitrophenyl ester. However, the recovered activity versus casein was under 20% at 50 °C, as it m
Externí odkaz:
https://doaj.org/article/87b6f62a8d0c4ed4a024b45723e50a79
Publikováno v:
Catalysts, Vol 8, Iss 11, p 511 (2018)
Eversa is an enzyme recently launched by Novozymes to be used in a free form as biocatalyst in biodiesel production. This paper shows for first time the immobilization of Eversa (a commercial lipase) on octyl and aminated agarose beads and the compar
Externí odkaz:
https://doaj.org/article/830493d88db2465790965b1b00672137
Autor:
El-Hocine Siar, Sara Arana-Peña, Oveimar Barbosa, Mohammed Nasreddine Zidoune, Roberto Fernandez-Lafuente
Publikováno v:
Catalysts, Vol 8, Iss 4, p 149 (2018)
Ficin extract has been immobilized on different 4% aminated-agarose beads. Using just ion exchange, immobilization yield was poor and expressed activity did not surpass 10% of the offered enzyme, with no significant effects on enzyme stability. The t
Externí odkaz:
https://doaj.org/article/2b08a47ab4904744982050c4cd09185f