Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Sara A.S.L. Rosa"'
Autor:
Pedro M. R. Paulo, Duarte M. F. Prazeres, Sara A.S.L. Rosa, Rui Oliveira-Silva, A. Rita Silva-Santos
Publikováno v:
ACS Applied Nano Materials. 4:14169-14177
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ce0cb20ded84d00c3e7e211ca58e8709
https://doi.org/10.1021/acsanm.1c03623
https://doi.org/10.1021/acsanm.1c03623
Publikováno v:
Vaccine
Vaccines are one of the most important tools in public health and play an important role in infectious diseases control. Owing to its precision, safe profile and flexible manufacturing, mRNA vaccines are reaching the stoplight as a new alternative to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13cdb790966b6136eb88cddba5bc71b3
https://pubmed.ncbi.nlm.nih.gov/33771389
https://pubmed.ncbi.nlm.nih.gov/33771389
Autor:
A. Rita Silva-Santos, Alexandra Wagner, Duarte M. F. Prazeres, Ana Azevedo, Sophie Gierak, Sara A.S.L. Rosa
Publikováno v:
Methods in Molecular Biology ISBN: 9781071608715
A method for the intermediate recovery of plasmid DNA (pDNA) from alkaline lysates is described that comprises differential isopropanol precipitation steps. In a first low-cut precipitation, a smaller amount of isopropanol (20% v/v) is used so that o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2735d1daa0c6d113b898f5c0cae916d3
https://doi.org/10.1007/978-1-0716-0872-2_8
https://doi.org/10.1007/978-1-0716-0872-2_8
Publikováno v:
Journal of Chromatography A. 1569:118-127
The aim of this work was to investigate the complex phenomena underlying the adsorption of an anti-human IL-8 (anti-IL8) monoclonal antibody (mAb) to m-aminophenylboronate (m-APBA) by Flow Microcalorimetry (FMC) and to understand the role of non-spec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbad77cc0136c337798a7345f41d717d
https://doi.org/10.1016/j.chroma.2018.07.050
https://doi.org/10.1016/j.chroma.2018.07.050
Autor:
Edith Espitia-Saloma, M. Raquel Aires-Barros, Isabel Campos-Pinto, Ana Azevedo, Oscar Aguilar, Miguel Arévalo-Rodríguez, Sara A.S.L. Rosa, Marco Rito-Palomares
Publikováno v:
Separation and Purification Technology. 173:129-134
Monoclonal antibodies (mAbs) are currently the most important class of recombinant protein therapeutics in the biotechnological and biopharmaceutical industry with more than 250 therapeutic mAbs currently undergoing clinical trials. High titer produc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a818a995ae8c88080b3bb43701e4c0e7
https://doi.org/10.1016/j.seppur.2016.09.017
https://doi.org/10.1016/j.seppur.2016.09.017
Autor:
Miguel de Almeida Fuzeta, Joaquim M. S. Cabral, Cecília R.C. Calado, Rodrigo Luz da Cunha, Ana Fernandes-Platzgummer, Rita de Cássia Silva, Sara A.S.L. Rosa, Ariana Azevedo, C.L. da Silva, C. Carvalho
Publikováno v:
Cytotherapy. 22:S48-S49
Background & Aim Currently, the production of extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSC) is performed in laborious and time-consuming static culture systems using serum-containing media, and their purification is throug
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fab85b226ad4014c68a3aecd6c1a60d4
https://doi.org/10.1016/j.jcyt.2020.03.058
https://doi.org/10.1016/j.jcyt.2020.03.058
Autor:
Maria Raquel Aires-Barros, Ana Azevedo, Alexandra Wagner, Sara A.S.L. Rosa, Cláudia Lobato da Silva, A.C. Dias-Cabral
Publikováno v:
Biotechnology journal. 14(10)
Phenylboronate chromatography has been employed for bioseparation applications though details concerning the mechanisms of interaction between the ligand and macromolecules remain widely unknown. Here, the phenomena underlying the adsorption of an an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27e84ec5f0f7cfd257de7bb081f00e37
https://pubmed.ncbi.nlm.nih.gov/31305007
https://pubmed.ncbi.nlm.nih.gov/31305007
Publikováno v:
Separation and Purification Technology. 160:43-50
This work addresses the feasibility of scaling-up phenylboronic acid (PBA) chromatography for the purification of human mAbs directly from Chinese Hamster Ovary cell culture supernatants. Column performance optimization regarding superficial velocity
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b0b9a8960216d7a5d481f454dd5635a9
https://doi.org/10.1016/j.seppur.2016.01.002
https://doi.org/10.1016/j.seppur.2016.01.002
Publikováno v:
Biochemical Engineering Journal. 104:27-33
In this work, an innovative and cost effective alternative to Protein A based on a commercially available HiTrap™ Heparin resin was evaluated as a first capture step for the purification of a monoclonal antibody from different CHO cell culture supe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0c0f4239bd7b67224557e53bec604f70
https://doi.org/10.1016/j.bej.2015.05.011
https://doi.org/10.1016/j.bej.2015.05.011
Publikováno v:
Separation and Purification Technology. 132:593-600
Monoclonal antibodies (mAbs) are the class of biopharmaceuticals showing the fastest growth in the pharmaceutical market. Therefore, an efficient manufacture process must be designed to meet the actual market needs. Diffusion-based devices such as pa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8ed14e8bb22cabb0eacc5ce6d038155f
https://doi.org/10.1016/j.seppur.2014.06.005
https://doi.org/10.1016/j.seppur.2014.06.005