Optimized Spatial Configuration of Heterogeneous Biocatalysts Maximizes Cell-Free Biosynthesis of ω-Hydroxy and ω-Amino Acids.

Autor: Santiago-Arcos J; Heterogeneous Biocatalysis Group, CIC biomaGUNE, Edificio Empresarial 'C', Paseo de Miramón 182, 20009 Donostia, Spain., Velasco-Lozano S; Heterogeneous Biocatalysis Group, CIC biomaGUNE, Edificio Empresarial 'C', Paseo de Miramón 182, 20009 Donostia, Spain.; Instituto de Síntesis Química y Catálisis Homogénea (ISQCH), CSIC-Universidad de Zaragoza, C/Pedro Cerbuna, 12, 50009 Zaragoza, Spain.; Aragonese Foundation for Research and Development (ARAID), 50018 Zaragoza, Spain., Diamanti E; Heterogeneous Biocatalysis Group, CIC biomaGUNE, Edificio Empresarial 'C', Paseo de Miramón 182, 20009 Donostia, Spain., Benítez-Mateos AI; Department of Chemistry, Biochemistry and Pharmaceutical Sciences, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland., Grajales-Hernández D; Heterogeneous Biocatalysis Group, CIC biomaGUNE, Edificio Empresarial 'C', Paseo de Miramón 182, 20009 Donostia, Spain., Paradisi F; Department of Chemistry, Biochemistry and Pharmaceutical Sciences, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland., López-Gallego F; Heterogeneous Biocatalysis Group, CIC biomaGUNE, Edificio Empresarial 'C', Paseo de Miramón 182, 20009 Donostia, Spain.; IKERBASQUE, Basque Foundation for Science, 48009 Bilbao, Spain.

Publikováno v: ACS sustainable chemistry & engineering [ACS Sustain Chem Eng] 2024 Jun 10; Vol. 12 (25), pp. 9474-9489. Date of Electronic Publication: 2024 Jun 10 (Print Publication: 2024).

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Engineered repeat proteins as scaffolds to assemble multi-enzyme systems for efficient cell-free biosynthesis.

Autor: Ledesma-Fernandez A; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramón 194, Donostia-San Sebastián, 20014, Spain., Velasco-Lozano S; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramón 194, Donostia-San Sebastián, 20014, Spain.; Institute of Chemical Synthesis and Homogeneous Catalysis (ISQCH-CSIC), University of Zaragoza, C/ Pedro Cerbuna, 12, 50009, Zaragoza, Spain.; Aragonese Foundation for Research and Development (ARAID), Zaragoza, Spain., Santiago-Arcos J; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramón 194, Donostia-San Sebastián, 20014, Spain., López-Gallego F; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramón 194, Donostia-San Sebastián, 20014, Spain. flopez@cicbiomagune.es.; Ikerbasque, Basque Foundation for Science, 48009, Bilbao, Spain. flopez@cicbiomagune.es., Cortajarena AL; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramón 194, Donostia-San Sebastián, 20014, Spain. alcortajarena@cicbiomagune.es.; Ikerbasque, Basque Foundation for Science, 48009, Bilbao, Spain. alcortajarena@cicbiomagune.es.

Publikováno v: Nature communications [Nat Commun] 2023 May 04; Vol. 14 (1), pp. 2587. Date of Electronic Publication: 2023 May 04.

Co-immobilization and colocalization of multi-enzyme systems for the cell-free biosynthesis of aminoalcohols

Autor: Velasco-Lozano, S, Santiago-Arcos, J, Mayoral, JA, López-Gallego, F.

The manufacturing of aminoalcohols is an appealing transformation for industrial biocatalysis due to their high value as building blocks in synthetic chemistry. By co‐immobilizing dehydrogenases, ω‐transaminases and oxidases on porous carriers,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______2659::0f7b3a5af459caa5dc3a686bc2083b24
https://zenodo.org/record/3740872

Intraparticle Kinetics Unveil Crowding and Enzyme Distribution Effects on the Performance of Cofactor-Dependent Heterogeneous Biocatalysts.

Autor: Diamanti E; Center for Cooperative Research in Biomaterials (CIC biomaGUNE)-Basque Research and Technology Alliance (BRTA), Paseo Miramón, 194, 20014 Donostia-San Sebastián, Spain., Santiago-Arcos J; Center for Cooperative Research in Biomaterials (CIC biomaGUNE)-Basque Research and Technology Alliance (BRTA), Paseo Miramón, 194, 20014 Donostia-San Sebastián, Spain., Grajales-Hernández D; Center for Cooperative Research in Biomaterials (CIC biomaGUNE)-Basque Research and Technology Alliance (BRTA), Paseo Miramón, 194, 20014 Donostia-San Sebastián, Spain., Czarnievicz N; Center for Cooperative Research in Biomaterials (CIC biomaGUNE)-Basque Research and Technology Alliance (BRTA), Paseo Miramón, 194, 20014 Donostia-San Sebastián, Spain., Comino N; Center for Cooperative Research in Biomaterials (CIC biomaGUNE)-Basque Research and Technology Alliance (BRTA), Paseo Miramón, 194, 20014 Donostia-San Sebastián, Spain., Llarena I; Center for Cooperative Research in Biomaterials (CIC biomaGUNE)-Basque Research and Technology Alliance (BRTA), Paseo Miramón, 194, 20014 Donostia-San Sebastián, Spain., Di Silvio D; Center for Cooperative Research in Biomaterials (CIC biomaGUNE)-Basque Research and Technology Alliance (BRTA), Paseo Miramón, 194, 20014 Donostia-San Sebastián, Spain., Cortajarena AL; Center for Cooperative Research in Biomaterials (CIC biomaGUNE)-Basque Research and Technology Alliance (BRTA), Paseo Miramón, 194, 20014 Donostia-San Sebastián, Spain.; IKERBASQUE, Basque Foundation for Science, Maria Diaz de Haro 3, 48013 Bilbao, Spain., López-Gallego F; Center for Cooperative Research in Biomaterials (CIC biomaGUNE)-Basque Research and Technology Alliance (BRTA), Paseo Miramón, 194, 20014 Donostia-San Sebastián, Spain.; IKERBASQUE, Basque Foundation for Science, Maria Diaz de Haro 3, 48013 Bilbao, Spain.

Publikováno v: ACS catalysis [ACS Catal] 2021 Dec 17; Vol. 11 (24), pp. 15051-15067. Date of Electronic Publication: 2021 Dec 01.