Zobrazeno 1 - 10
of 44
pro vyhledávání: '"Santiago M. Di Pietro"'
Autor:
Andrew K. Lamb, Santiago M. Di Pietro
Publikováno v:
STAR Protocols, Vol 3, Iss 2, Pp 101323- (2022)
Summary: Chemically induced dimerization (CID) is a useful tool for artificially inducing protein-protein interactions. Although CID has been used extensively for live-cell microscopy applications in mammalian systems, it is rarely utilized in yeast
Externí odkaz:
https://doaj.org/article/56a679cdc0a94401b7aefb1348e5eae9
Publikováno v:
Blood Advances, Vol 3, Iss 17, Pp 2617-2626 (2019)
Abstract: Platelet α-granules play important roles in platelet function. They contain hundreds of proteins that are synthesized by the megakaryocyte or taken up by endocytosis. The trafficking pathways that mediate platelet α-granule biogenesis are
Externí odkaz:
https://doaj.org/article/3ed4cb9288e14957b9e47b06125dc6d8
Publikováno v:
Platelets, Vol 28, Iss 2, Pp 138-146 (2017)
Platelet dense granules (DGs) are membrane bound compartments that store polyphosphate and small molecules such as ADP, ATP, Ca2+, and serotonin. The release of DG contents plays a central role in platelet aggregation to form a hemostatic plug. Accor
Externí odkaz:
https://doaj.org/article/281b15913724464ab70e0a34bc3e79d0
Publikováno v:
Blood
Platelet α-granules regulate hemostasis and myriad other physiological processes, but their biogenesis is unclear. Mutations in only 3 proteins are known to cause α-granule defects and bleeding disorders in humans. Two such proteins, VPS16B and VPS
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1399643ba50e71ddbf8269925729e781
https://doi.org/10.1182/blood.2021012056
https://doi.org/10.1182/blood.2021012056
Autor:
Alexandra Unapanta, Farbod Shavarebi, Jacob Porath, Carson Balen, Albert Nguyen, Josh Tseng, Yiyi Shen, Michelle Liu, Pawel Lis, Santiago M. Di Pietro, Annie Hiniker
Point mutations in LRRK2 cause Parkinson’s Disease and augment LRRK2’s kinase activity. However, cellular pathways that enhance LRRK2 kinase function have not been identified. While overexpressed Rab29 draws LRRK2 to Golgi membranes to increase L
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::916c1b5deaeb305ea7014b9530e413bf
https://doi.org/10.1101/2022.06.20.496629
https://doi.org/10.1101/2022.06.20.496629
Publikováno v:
The Journal of biological chemistry. 298(12)
Mutations in C10orf11 (oculocutaneous albinism type 7 [OCA7]) cause OCA, a disorder that presents with hypopigmentation in skin, eyes, and hair. The OCA7 pathophysiology is unknown, and there is virtually no information on the OCA7 protein and its ce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cbfdc84e702c8ca8e7ad44b9972d790b
https://pubmed.ncbi.nlm.nih.gov/36334630
https://pubmed.ncbi.nlm.nih.gov/36334630
Publikováno v:
Journal of Cell Science.
Endocytosis of plasma membrane proteins is mediated by their interaction with adaptor proteins. Conversely, emerging evidence suggests adaptor protein recruitment to the plasma membrane may depend on binding to endocytic cargo. To test this idea we a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fa9d2c0b2ab0357c1f2d1627e689569e
https://doi.org/10.1242/jcs.247684
https://doi.org/10.1242/jcs.247684
Publikováno v:
Traffic. 19:446-462
Clathrin mediated endocytosis is a fundamental transport pathway that depends on numerous protein-protein interactions. Testing the importance of the adaptor protein-clathrin interaction for coat formation and progression of endocytosis in vivo has b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae01c3777975c9e85eebd4ead309aa27
https://doi.org/10.1111/tra.12563
https://doi.org/10.1111/tra.12563
Publikováno v:
Molecular Biology of the Cell
Clathrin- and actin-mediated endocytosis is a fundamental process in eukaryotic cells. Previously, we discovered Tda2 as a new yeast dynein light chain that works with Aim21 to regulate actin assembly during endocytosis. Here, we show Tda2 functions
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d7b08649bb481612967fb2f6458f5db
https://doi.org/10.1091/mbc.e21-01-0032
https://doi.org/10.1091/mbc.e21-01-0032
Autor:
Santiago M. Di Pietro, Seth McDonald, Olve B. Peersen, Kristen B. Farrell, Andrew K. Lamb, Colette R. Worcester
Publikováno v:
The Journal of Cell Biology
Actin-capping protein is a key component of the actin cytoskeleton at sites of clathrin-mediated endocytosis. Farrell et al. show that a newly discovered component of the endocytic machinery belongs to the dynein light chain family and regulates the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c753c99e0dfba192e2de6be3346165bf
https://doi.org/10.1083/jcb.201604123
https://doi.org/10.1083/jcb.201604123