Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Sanne E Klompe"'
Publikováno v:
Nucleic Acids Research. 51:4519-4535
CRISPR-associated transposases (CASTs) direct DNA integration downstream of target sites using the RNA-guided DNA binding activity of nuclease-deficient CRISPR-Cas systems. Transposition relies on several key protein-protein and protein-DNA interacti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::25a2c397300bf985691ae1df81a64156
https://doi.org/10.1093/nar/gkad270
https://doi.org/10.1093/nar/gkad270
Autor:
George D. Lampe, Rebeca T. King, Tyler S. Halpin-Healy, Sanne E. Klompe, Marcus I. Hogan, Phuc Leo H. Vo, Stephen Tang, Alejandro Chavez, Samuel H. Sternberg
Publikováno v:
Nature Biotechnology.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::23f5be4d30797441e7f22401a637dca6
https://doi.org/10.1038/s41587-023-01748-1
https://doi.org/10.1038/s41587-023-01748-1
Autor:
Diego R. Gelsinger, Phuc Leo H. Vo, Sanne E. Klompe, Carlotta Ronda, Harris Wang, Samuel H. Sternberg
Publikováno v:
bioRxiv
CRISPR-associated transposons (CASTs) have the potential to transform the technology landscape for kilobase-scale genome engineering, by virtue of their ability to integrate large genetic payloads with high accuracy, easy programmability, and no requ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::614f99902e87d153625df3e1e92083d2
https://europepmc.org/articles/PMC10055292/
https://europepmc.org/articles/PMC10055292/
Autor:
George D. Lampe, Rebeca T. King, Tyler S. Halpin-Healy, Sanne E. Klompe, Marcus I. Hogan, Phuc Leo H. Vo, Stephen Tang, Alejandro Chavez, Samuel H. Sternberg
Publikováno v:
bioRxiv
Traditional genome-editing reagents such as CRISPR-Cas9 achieve targeted DNA modification by introducing double-strand breaks (DSBs), thereby stimulating localized DNA repair by endogenous cellular repair factors. While highly effective at generating
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dedf336c5dfa340acb16ed7115aea378
https://europepmc.org/articles/PMC10055298/
https://europepmc.org/articles/PMC10055298/
CRISPR-associated transposons (CASTs) direct DNA integration downstream of target sites using the RNA-guided DNA binding activity of nuclease-deficient CRISPR-Cas systems. Transposition relies on several key protein-protein and protein-DNA interactio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1a3555acbf3f66a7f9145015afe4382c
https://doi.org/10.1101/2023.01.19.524723
https://doi.org/10.1101/2023.01.19.524723
Publikováno v:
Digital.CSIC. Repositorio Institucional del CSIC
instname
Digital.CSIC: Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)
instname
Digital.CSIC: Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)
Bacteria use adaptive immune systems encoded by CRISPR and Cas genes to maintain genomic integrity when challenged by pathogens and mobile genetic elements1,2,3. Type I CRISPR–Cas systems typically target foreign DNA for degradation via joint actio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6535059ddab9916692f380959b18f350
https://doi.org/10.1038/s41586-019-1849-0
https://doi.org/10.1038/s41586-019-1849-0
Publikováno v:
Nature. 571:219-225
Conventional CRISPR-Cas systems maintain genomic integrity by leveraging guide RNAs for the nuclease-dependent degradation of mobile genetic elements, including plasmids and viruses. Here we describe a notable inversion of this paradigm, in which bac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7c579a9cc035727c290c08e3fdcee601
https://doi.org/10.1038/s41586-019-1323-z
https://doi.org/10.1038/s41586-019-1323-z
Autor:
Peter Cameron, Mckenzi S. Toh, Steven B. Kanner, John van der Oost, Scott Gradia, Bastien Vidal, Carolyn Williams, Samuel H. Sternberg, Lynda M. Banh, Alexandra M. Lied, Paul Daniel Donohoue, David B. Nyer, Chris R. Fuller, B. Kohrs, Mary M. Coons, Matthew J. Irby, Chun Han Lin, Leslie S. Edwards, Arthur L.G. Owen, Tim Künne, Tomer Rotstein, Sanne E. Klompe, Rachel Kennedy, Euan M. Slorach, Andrew May, Stan J. J. Brouns, Stephen C. Smith
Publikováno v:
Nature Biotechnology
Nature Biotechnology, 37, 1471-1477
Nature Biotechnology 37 (2019)
Nature Biotechnology, 37, 1471-1477
Nature Biotechnology 37 (2019)
Type I CRISPR–Cas systems are the most abundant adaptive immune systems in bacteria and archaea1,2. Target interference relies on a multi-subunit, RNA-guided complex called Cascade3,4, which recruits a trans-acting helicase-nuclease, Cas3, for targ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bca5f5a349524ce734ff58581aea866d
https://doi.org/10.1038/s41587-019-0310-0
https://doi.org/10.1038/s41587-019-0310-0
Publikováno v:
Nature. 585(7825)
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6b955a5d7bdedbf840e265a4d02fe3f
https://pubmed.ncbi.nlm.nih.gov/31853065
https://pubmed.ncbi.nlm.nih.gov/31853065
Autor:
Sanne E. Klompe, Phuc Leo H. Vo, Ethan E. Chen, Harris H. Wang, Christopher Acree, Carlotta Ronda, Samuel H. Sternberg
Tn7-like transposons are pervasive mobile genetic elements in bacteria that mobilize using heteromeric transposase complexes comprising distinct targeting modules. We recently described a Tn7-like transposon fromVibrio choleraethat employs a Type I-F
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a47950d02ed968d8aa9f00a88a568c44
https://doi.org/10.1101/2020.07.17.209452
https://doi.org/10.1101/2020.07.17.209452