Zobrazeno 1 - 10
of 52
pro vyhledávání: '"Sang-Wook Cha"'
Autor:
Zachary Agricola, Sang-Wook Cha
Publikováno v:
BioTechniques, Vol 61, Iss 5, Pp 263-268 (2016)
Xenopus embryo yolk proteins present a serious barrier to fluorescence microscopy. Previously, in situ assays of gene transcripts in whole embryos was limited to the use of chromogenic alkaline phosphatase substrates, which restricted researchers' ab
Externí odkaz:
https://doaj.org/article/b317549af6834e37bff64a4836388b27
Autor:
Jeeyeon Cha, Amanda Bartos, Craig Park, Xiaofei Sun, Yingju Li, Sang-Wook Cha, Rieko Ajima, Hsin-Yi Henry Ho, Terry P. Yamaguchi, Sudhansu K. Dey
Publikováno v:
Cell Reports, Vol 8, Iss 2, Pp 382-392 (2014)
Embryo homing and implantation occur within a crypt (implantation chamber) at the antimesometrial (AM) pole along the uterus. The mechanism by which this is achieved is not known. Here, we show that villi-like epithelial projections from the main ute
Externí odkaz:
https://doaj.org/article/14bfc18ed32c4ffd9246a11e469215d3
Autor:
Emmanuel Tadjuidje, Matthew Kofron, Adnan Mir, Christopher Wylie, Janet Heasman, Sang-Wook Cha
Publikováno v:
Open Biology, Vol 6, Iss 8 (2016)
Nodal class TGF-β signalling molecules play essential roles in establishing the vertebrate body plan. In all vertebrates, nodal family members have specific waves of expression required for tissue specification and axis formation. In Xenopus laevis,
Externí odkaz:
https://doaj.org/article/8edb9acd2fd547aebf315884b8327bd8
Publikováno v:
PLoS ONE, Vol 8, Iss 10, p e76854 (2013)
During early vertebrate development, epithelial cells establish and maintain apicobasal polarity, failure of which can cause developmental defects or cancer metastasis. This process has been mostly studied in simple epithelia that have only one layer
Externí odkaz:
https://doaj.org/article/7e0583a7ff5d4a6f8de8ebd28102244c
Publikováno v:
PLoS ONE, Vol 7, Iss 7, p e41782 (2012)
Foxi1e is a zygotic transcription factor that is essential for the expression of early ectodermal genes. It is expressed in a highly specific pattern, only in the deep cell layers of the animal hemisphere, and in a mosaic pattern in which expressing
Externí odkaz:
https://doaj.org/article/37e9bd1ef2a2420db3a59e39cf1bf9e2
Publikováno v:
PLoS ONE, Vol 7, Iss 6, p e38756 (2012)
Alpha (α)-E-catenin is a component of the cadherin complex, and has long been thought to provide a link between cell surface cadherins and the actin skeleton. More recently, it has also been implicated in mechano-sensing, and in the control of tissu
Externí odkaz:
https://doaj.org/article/227a6dc6f0db458883b86af568e7ae26
Autor:
Sung-Young Lee, Jaeho Yoon, Hyun-Shik Lee, Yoo-Seok Hwang, Sang-Wook Cha, Chul-Ho Jeong, Jong-Il Kim, Jae-Bong Park, Jae-Yong Lee, SungChan Kim, Mae Ja Park, Zigang Dong, Jaebong Kim
Publikováno v:
PLoS ONE, Vol 6, Iss 7, p e21796 (2011)
BACKGROUND:Activator protein-1 (AP-1) is a mediator of BMP or FGF signaling during Xenopus embryogenesis. However, specific role of AP-1 in activin signaling has not been elucidated during vertebrate development. METHODOLOGY/PRINCIPAL FINDINGS:We pro
Externí odkaz:
https://doaj.org/article/67bb874c3bd942d0bac7797164de3c09
Autor:
Hyun-Shik Lee, Sung-Young Lee, Hyosang Lee, Yoo-Seok Hwang, Sang-Wook Cha, Soochul Park, Jae-Yong Lee, Jae-Bong Park, Sungchan Kim, Mae Ja Park, Jaebong Kim
Publikováno v:
PLoS ONE, Vol 6, Iss 8, p e22621 (2011)
Xvent homeobox genes encode transcription factors that repress organizer genes and are essential for dorsoventral specification during early embryogenesis in Xenopus. In contrast to the Xvent-2 gene subfamily, Xvent-1 subfamily members, including PV.
Externí odkaz:
https://doaj.org/article/79101688ad3f4c209e0a148dfa7999f7
Publikováno v:
Cold Spring Harbor protocols. 2022(12)
We describe a step-by-step procedure to perform homology-directed repair (HDR)-mediated precise gene editing in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::35c556a2aef654984a8d56b138de66c6
https://pubmed.ncbi.nlm.nih.gov/35953242
https://pubmed.ncbi.nlm.nih.gov/35953242
Autor:
Sang-Wook, Cha
Publikováno v:
Cold Spring Harbor protocols. 2022(6)
In CRISPR-Cas9 genome editing, double-strand DNA breaks (DSBs) primarily undergo repair through nonhomologous end joining (NHEJ), which produces insertion or deletion of random nucleotides within the targeted region (indels). As a result, frameshift
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::96092fc43be9a812491d421688451dda
https://pubmed.ncbi.nlm.nih.gov/34244351
https://pubmed.ncbi.nlm.nih.gov/34244351