Zobrazeno 1 - 10
of 110
pro vyhledávání: '"Sang‐Ging Ong"'
Autor:
Chengdong Zhang, Yuan Yang, Tao Qi, Yuening Zhang, Linghui Hou, Jingjing Wei, Jingcheng Yang, Leming Shi, Sang-Ging Ong, Hongyan Wang, Hui Wang, Bo Yu, Yongming Wang
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-11 (2023)
Abstract Due to the tolerance of mismatches between gRNA and targeting sequence, base editors frequently induce unwanted Cas9-dependent off-target mutations. Here, to develop models to predict such off-targets, we design gRNA-off- target pairs for ad
Externí odkaz:
https://doaj.org/article/28a94f556575445abd86aca74c30d663
Autor:
Shubhi Srivastava, Priyanka Gajwani, Jordan Jousma, Hiroe Miyamoto, Youjeong Kwon, Arundhati Jana, Peter T. Toth, Gege Yan, Sang-Ging Ong, Jalees Rehman
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)
Abstract Chemotherapy-induced cardiac damage remains a leading cause of death amongst cancer survivors. Anthracycline-induced cardiotoxicity is mediated by severe mitochondrial injury, but little is known about the mechanisms by which cardiomyocytes
Externí odkaz:
https://doaj.org/article/3304c83eef7a4c07ac6642b821364c12
Autor:
Ruifan Wu, Jooman Park, Yanyu Qian, Zuoxiao Shi, Ruoci Hu, Yexian Yuan, Shaolei Xiong, Zilai Wang, Gege Yan, Sang-Ging Ong, Qing Song, Zhenyuan Song, Abeer M. Mahmoud, Pingwen Xu, Congcong He, Robert W. Arpke, Michael Kyba, Gang Shu, Qingyan Jiang, Yuwei Jiang
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-17 (2023)
Abstract A potential therapeutic target to curb obesity and diabetes is thermogenic beige adipocytes. However, beige adipocytes quickly transition into white adipocytes upon removing stimuli. Here, we define the critical role of cyclin dependent kina
Externí odkaz:
https://doaj.org/article/0357368b73a6464c98e1c6cffa3af7b9
Publikováno v:
Cell & Bioscience, Vol 12, Iss 1, Pp 1-28 (2022)
Abstract Cancer is one of the leading causes of morbidity and mortality worldwide. Significant improvements in the modern era of anticancer therapeutic strategies have increased the survival rate of cancer patients. Unfortunately, cancer survivors ha
Externí odkaz:
https://doaj.org/article/7454a07776314e57b81e464054ff2db0
Autor:
Shuai Wang, Chen Tao, Huilin Mao, Linghui Hou, Yao Wang, Tao Qi, Yuan Yang, Sang-Ging Ong, Shijun Hu, Renjie Chai, Yongming Wang
Publikováno v:
PLoS Biology, Vol 20, Iss 11, p e3001897 (2022)
Due to different nucleotide preferences at target sites, no single Cas9 is capable of editing all sequences. Thus, this highlights the need to establish a Cas9 repertoire covering all sequences for efficient genome editing. Cas9s with simple protospa
Externí odkaz:
https://doaj.org/article/baee577b55524ed0813dc7d4618d843c
Autor:
Edit Gara, Sang-Ging Ong, Johannes Winkler, Katrin Zlabinger, Dominika Lukovic, Bela Merkely, Maximilian Y. Emmert, Petra Wolint, Simon P. Hoerstrup, Mariann Gyöngyösi, Joseph C. Wu, Noemi Pavo
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 10 (2022)
Recent preclinical investigations and clinical trials with stem cells mostly studied bone-marrow-derived mononuclear cells (BM-MNCs), which so far failed to meet clinically significant functional study endpoints. BM-MNCs containing small proportions
Externí odkaz:
https://doaj.org/article/70555f8610974d02a82f41484bd3bc49
Autor:
Olivia T. Ly, Hanna Chen, Grace E. Brown, Liang Hong, Xinge Wang, Yong Duk Han, Mahmud Arif Pavel, Arvind Sridhar, Mark Maienschein-Cline, Brandon Chalazan, Sang-Ging Ong, Khaled Abdelhady, Malek Massad, Lona Ernst Rizkallah, Jalees Rehman, Salman R. Khetani, Dawood Darbar
Publikováno v:
JCI Insight, Vol 7, Iss 7 (2022)
Human induced pluripotent stem cell–derived cardiomyocytes (iPSC-CMs) can model heritable arrhythmias to personalize therapies for individual patients. Although atrial fibrillation (AF) is a leading cause of cardiovascular morbidity and mortality,
Externí odkaz:
https://doaj.org/article/2cbefd4c83254f4fb0d6f8d93e7817d4
Autor:
Youjeong Kwon, Sarath Babu Nukala, Shubhi Srivastava, Hiroe Miyamoto, Nur Izzah Ismail, Jordan Jousma, Jalees Rehman, Sang-Bing Ong, Won Hee Lee, Sang-Ging Ong
Publikováno v:
Stem Cell Research & Therapy, Vol 11, Iss 1, Pp 1-5 (2020)
Abstract Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global pandemic. The prevalence/severity of COVID-19 is higher among patients with cardiovascular risk factors. Despite the e
Externí odkaz:
https://doaj.org/article/b91833c0c5b348b49892c1c3e3da0941
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 9 (2021)
CRISPR/Cas9 nucleases hold great potential for gene therapy, but they frequently induce unwanted off-target cleavage. We previously developed a GFP activation assay for detection of DNA cleavage in cells. Here, we demonstrate two novel applications o
Externí odkaz:
https://doaj.org/article/ed723177ffba47588f6be8e71deee98e
Autor:
Bei Wang, Zishi Wang, Daqi Wang, Baolong Zhang, Sang-Ging Ong, Mingqing Li, Wenqiang Yu, Yongming Wang
Publikováno v:
Journal of Biological Engineering, Vol 13, Iss 1, Pp 1-13 (2019)
Abstracts Background CRISPR/Cas9 system is a powerful tool for knocking out genes in cells. However, genes essential for cell survival cannot be directly knocked out. Traditionally, generation of conditional knockout cells requires multiple steps. Re
Externí odkaz:
https://doaj.org/article/35906630a32b40e5afb1e0f6f3fdfd6e