A simple method to determine changes in the affinity between HisF and HisH in the Imidazole Glycerol Phosphate Synthase heterodimer.

Autor: Vitor M Almeida, J Patrick Loria, Sandro R Marana

Publikováno v: PLoS ONE, Vol 17, Iss 4, p e0267536 (2022)

The bi-enzyme HisF-HisH heterodimer is part of the pathway that produces histidine and purines in bacteria and lower eukaryotes, but it is absent in mammals. This heterodimer has been largely studied probing the basis of the allosteric effects and th

Externí odkaz: https://doaj.org/article/099cdaed7ad743b18e8d4d2d85eb85e3

Search for independent (β/α)4 subdomains in a (β/α)8 barrel β-glucosidase.

Autor: Vitor M Almeida, Maira A Frutuoso, Sandro R Marana

Publikováno v: PLoS ONE, Vol 13, Iss 1, p e0191282 (2018)

Proteins that fold as (β/α)8 barrels are thought to have evolved from half-barrels that underwent duplication and fusion events. The evidence is particularly clear for small barrels, which have almost identical halves. Additionally, computational c

Externí odkaz: https://doaj.org/article/359e85f6f0a64201ac1e56101ee134ab

Using the Amino Acid Network to Modulate the Hydrolytic Activity of β-Glycosidases.

Autor: Fábio K Tamaki, Diorge P Souza, Valquiria P Souza, Cecilia M Ikegami, Chuck S Farah, Sandro R Marana

Publikováno v: PLoS ONE, Vol 11, Iss 12, p e0167978 (2016)

The active site residues in GH1 β-glycosidases are compartmentalized into 3 functional regions, involved in catalysis or binding of glycone and aglycone motifs from substrate. However, it still remains unclear how residues outside the active site mo

Externí odkaz: https://doaj.org/article/d650f3c1f1cd4f25addd69b7cf4d9133

Sets of covariant residues modulate the activity and thermal stability of GH1 β-glucosidases.

Autor: Fábio K Tamaki, Larissa C Textor, Igor Polikarpov, Sandro R Marana

Publikováno v: PLoS ONE, Vol 9, Iss 5, p e96627 (2014)

The statistical coupling analysis of 768 β-glucosidases from the GH1 family revealed 23 positions in which the amino acid frequencies are coupled. The roles of these covariant positions in terms of the properties of β-glucosidases were investigated

Externí odkaz: https://doaj.org/article/e6912e8aa6554dbaaa5cc7c75985a4b2

Molecular Dynamics Reveals Complex Compensatory Effects of Ionic Strength on the Severe Acute Respiratory Syndrome Coronavirus 2 Spike/Human Angiotensin-Converting Enzyme 2 Interaction

Autor: Chuck S. Farah, Cristiane R. Guzzo, Anacleto Silva de Souza, Jose D. Rivera, Henning Ulrich, Pingju Ge, Roberto Kopke Salinas, Vitor Medeiros Almeida, Robson Francisco de Souza, Sandro R. Marana

Publikováno v: The Journal of Physical Chemistry Letters
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

The SARS-CoV-2 pandemic has already killed more than one million people worldwide. To gain entry, the virus uses its Spike protein to bind to host hACE-2 receptors on the host cell surface and mediate fusion between viral and cell membranes. As initi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3491d1ba25cffc44c0ec011aaa13445f
https://doi.org/10.1021/acs.jpclett.0c02602