Zobrazeno 1 - 10 of 14 pro vyhledávání: '"Sandrine Magnard"'
1
The non-Newtonian behavior of detergents during concentration is increased by macromolecules, in trans, and results in their over-concentration
Autor: Alexia Gobet, Veronica Zampieri, Sandrine Magnard, Eva Pebay-Peyroula, Pierre Falson, Vincent Chaptal
Publikováno v: Biochimie
Biochimie, In press, ⟨10.1016/j.biochi.2022.09.004⟩
International audience; Concentration of pure membrane proteins in detergent solution results in detergent concentration, albeit in unknown amounts. This phenomenon is observed in every lab working on membrane proteins, but has seldom been investigat
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30328a25d115eb77677b293401ecaf5a
https://pubmed.ncbi.nlm.nih.gov/36087644
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2
Overexpression of the ABC Transporter BmrA Within Intracellular Caveolae in Escherichia coli
Autor: Alexia, Gobet, Christine, Jaxel, Sandrine, Magnard, Manuel, Garrigos, Stéphane, Orlowski, Nadège, Jamin, Pierre, Falson, Vincent, Chaptal
Publikováno v: Methods in molecular biology (Clifton, N.J.). 2507
We describe here the overproduction and oriented membrane insertion of membrane protein inside intracellular vesicles named heterologous caveolae within E. coli. The method is described with BmrA, a multidrug efflux pump from Bacillus subtilis. BmrA
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d43699b9a1c2a46e333e33408c7b6a58
https://pubmed.ncbi.nlm.nih.gov/35773578
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3
Production and Purification of a GFP-Tagged ABC Transporter CaCdr1p
Autor: Jorgaq, Pata, Alexis, Moreno, Sandrine, Magnard, Atanu, Banerjee, Rajendra, Prasad, Pierre, Falson
Publikováno v: Methods in molecular biology (Clifton, N.J.). 2507
The production and purification are the first steps required in any functional or structural study of a protein of interest. In the case of membrane proteins, these tasks can be difficult due to low expression levels and the necessity to extract them
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::be10ec225134471c47c922595f01d2df
https://pubmed.ncbi.nlm.nih.gov/35773582
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4
Substrate-bound and substrate-free outward-facing structures of a multidrug ABC exporter
Autor: Vincent Chaptal, Veronica Zampieri, Benjamin Wiseman, Cédric Orelle, Juliette Martin, Kim-Anh Nguyen, Alexia Gobet, Margot Di Cesare, Sandrine Magnard, Waqas Javed, Jad Eid, Arnaud Kilburg, Marine Peuchmaur, Julien Marcoux, Luca Monticelli, Martin Hogbom, Guy Schoehn, Jean-Michel Jault, Ahcène Boumendjel, Pierre Falson
Publikováno v: Science Advances
Science Advances, 2022, 8 (4), pp.eabg9215. ⟨10.1126/sciadv.abg9215⟩
Science Advances, American Association for the Advancement of Science (AAAS), 2022, 8 (4), pp.eabg9215. ⟨10.1126/sciadv.abg9215⟩
Multidrug ABC transporters translocate drugs across membranes by a mechanism for which the molecular features of drug release are so far unknown. Here, we resolved three ATP-Mg 2+ –bound outward-facing conformations of the Bacillus subtilis (homodi
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12388d8dd4050b1eea0a1e4b562b5bd3
https://pubmed.ncbi.nlm.nih.gov/35080979
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6
Overexpression of the ABC Transporter BmrA Within Intracellular Caveolae in Escherichia coli
Autor: Alexia Gobet, Christine Jaxel, Sandrine Magnard, Manuel Garrigos, Stéphane Orlowski, Nadège Jamin, Pierre Falson, Vincent Chaptal
Publikováno v: Methods in Molecular Biology ISBN: 9781071623671
Heterologous Expression of Membrane Proteins
Heterologous Expression of Membrane Proteins, 2507, Springer US, pp.79-89, 2022, Methods in Molecular Biology, ⟨10.1007/978-1-0716-2368-8_5⟩
International audience; We described here the overproduction and oriented membrane insertion of membrane protein inside intracellular vesicles named heterologous caveolae within E. coli. The method is described with BmrA, a multidrug efflux pump from
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::800e8d36c3deabe6bebc074035f222bc
https://doi.org/10.1007/978-1-0716-2368-8_5
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8
Glycosyl-Substituted Dicarboxylates as Detergents for the Extraction, Overstabilization, and Crystallization of Membrane Proteins
Autor: Sandrine Magnard, Pierre Falson, Saravanan Mounien, Sébastien Igonet, Veronica Zampieri, Romain Haudecoeur, Cédric Boyère, Marine Peuchmaur, Kim-Anh Nguyen, Anass Jawhari, Vincent Chaptal, Ahcène Boumendjel, Ikram Benammar
Publikováno v: Angewandte Chemie International Edition
Angewandte Chemie International Edition, Wiley-VCH Verlag, 2018, 57 (11), pp.2948-2952. ⟨10.1002/anie.201713395⟩
To tackle the problems associated with membrane protein (MP) instability in detergent solutions, we designed a series of glycosyl-substituted dicarboxylate detergents (DCODs) in which we optimized the polar head to clamp the membrane domain by includ
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::182233eebcd281cc95b84fe6e463d066
https://hal.archives-ouvertes.fr/hal-02329303
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9
Quantification of Detergents Complexed with Membrane Proteins
Autor: Martin Picard, Jean-Michel Jault, Renaud Wagner, Olivier Bornert, Pierre Falson, Cédric Orelle, Morgane Agez, Vincent Chaptal, Cédric Montigny, Marc le Maire, Charlène Prier, Arnaud Kilburg, Luca Monticelli, H. Ronald Kaback, Eva Pebay-Peyroula, Anass Jawhari, Stéphanie Ravaud, Sandrine Magnard, Frédéric Delolme, Isabelle Broutin
Publikováno v: Scientific Reports
Scientific Reports, Nature Publishing Group, 2017, 7, ⟨10.1038/srep41751⟩
Scientific Reports, Nature Publishing Group, 2017, 7 (1), ⟨10.1038/srep41751⟩
Scientific reports, vol 7, iss 1
Scientific Reports, 2017, 7 (1), ⟨10.1038/srep41751⟩
Scientific Reports, 2017, 7, ⟨10.1038/srep41751⟩
Scientific Reports, Nature Publishing Group, 2017, 7, 〈10.1038/srep41751〉
International audience; Most membrane proteins studies require the use of detergents, but because of the lack of a general, accurate and rapid method to quantify them, many uncertainties remain that hamper proper functional and structural data analys
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43a4f0ffcfdb3af2f4f620c917d5da92
http://www.nature.com/articles/srep41751
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10
Collateral sensitivity of resistant MRP1-overexpressing cells to flavonoids and derivatives through GSH efflux
Autor: Pierre-Alain Carrupt, Sandrine Magnard, Attilio Di Pietro, Claudia A. Simões-Pires, Raphaël Terreux, Estelle Genoux-Bastide, Hélène Baubichon-Cortay, Doriane Lorendeau, Ahcène Boumendjel, Lauriane Dury, Florine Lecerf-Schmidt
Publikováno v: Biochemical Pharmacology. 90:235-245
The multidrug resistance protein 1 (MRP1) is involved in multidrug resistance of cancer cells by mediating drug efflux out of cells, often in co-transport with glutathione (GSH). GSH efflux mediated by MRP1 can be stimulated by verapamil. In cells ov
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e44e4d408d7eab20ce19b5e977e5f542
https://doi.org/10.1016/j.bcp.2014.05.017
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