Structural and Biochemical Characterization of Mycobacterium tuberculosis Zinc SufU-SufS Complex

Autor: Ingie Elchennawi, Philippe Carpentier, Christelle Caux, Marine Ponge, Sandrine Ollagnier de Choudens

Publikováno v: Biomolecules, Vol 13, Iss 5, p 732 (2023)

Iron-sulfur (Fe-S) clusters are inorganic prosthetic groups in proteins composed exclusively of iron and inorganic sulfide. These cofactors are required in a wide range of critical cellular pathways. Iron-sulfur clusters do not form spontaneously in

Externí odkaz: https://doaj.org/article/3b9e705d98c346e7a8887465f76ce5c2

Iron–Sulfur Clusters toward Stresses: Implication for Understanding and Fighting Tuberculosis

Autor: Ingie Elchennawi, Sandrine Ollagnier de Choudens

Publikováno v: Inorganics, Vol 10, Iss 10, p 174 (2022)

Tuberculosis (TB) remains the leading cause of death due to a single pathogen, accounting for 1.5 million deaths annually on the global level. Mycobacterium tuberculosis, the causative agent of TB, is persistently exposed to stresses such as reactive

Externí odkaz: https://doaj.org/article/1ca98d6ca55248bab0b75f32078b1818

An early origin of iron–sulfur cluster biosynthesis machineries before Earth oxygenation

Autor: Pierre Simon Garcia, Francesca D’Angelo, Sandrine Ollagnier de Choudens, Macha Dussouchaud, Emmanuelle Bouveret, Simonetta Gribaldo, Frédéric Barras

Publikováno v: Nature Ecology & Evolution
Nature Ecology & Evolution, 2022, 6, pp.1564-1572. ⟨10.1038/s41559-022-01857-1⟩

International audience; Iron–sulfur (Fe–S) clusters are ubiquitous cofactors essential for life. It is largely thought that the emergence of oxygenic photosynthesis and progressive oxygenation of the atmosphere led to the origin of multiprotein m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5756309b7dbc9941c65865869cf0347
https://hal.science/hal-03780728/document

Cellular assays identify barriers impeding iron-sulfur enzyme activity in a non-native prokaryotic host

Autor: Helena Shomar, Pierre Simon Garcia, Elena Fernández-Fueyo, Francesca D'Angelo, Martin Pelosse, Rita Rebelo Manuel, Ferhat Büke, Siyi Liu, Niels van den Broek, Nicolas Duraffourg, Carol de Ram, Martin Pabst, Emmanuelle Bouveret, Simonetta Gribaldo, Béatrice Py, Sandrine Ollagnier de Choudens, Frédéric Barras, Gregory Bokinsky

Publikováno v: eLife, 11
eLife
eLife, 2022, 11, pp.e70936. ⟨10.7554/eLife.70936⟩

Iron-sulfur (Fe-S) clusters are ancient and ubiquitous protein cofactors and play irreplaceable roles in many metabolic and regulatory processes. Fe-S clusters are built and distributed to Fe-S enzymes by dedicated protein networks. The core componen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e7b3c370807a29232eb840366489f3e
https://doi.org/10.7554/elife.70936

Transient Formation of a Second Active Site Cavity during Quinolinic Acid Synthesis by NadA

Autor: Sandrine Ollagnier de Choudens, Lydie Martin, Roman Rohac, Juan-Carlos Fontecilla-Camps, Patricia Amara, Anne Volbeda, Hind Basbous

Publikováno v: ACS Chemical Biology
ACS Chemical Biology, 2021, 16 (11), pp.2423-2433. ⟨10.1021/acschembio.1c00541⟩
ACS Chemical Biology, American Chemical Society, 2021, 16 (11), pp.2423-2433. ⟨10.1021/acschembio.1c00541⟩
ACS Chemical Biology, American Chemical Society, In press, ⟨10.1021/acschembio.1c00541⟩

International audience; : Quinolinate synthase, also called NadA, is a [4Fe-4S]-containing enzyme that uses what is probably the oldest pathway to generate quinolinic acid (QA), the universal precursor of the biologically essential cofactor nicotinam

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b5aec2bfdf6a6c9d6fd290df80c8f334
https://hal.science/hal-03383427/file/acschembio.1c00541.pdf