Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Sandra Scagliarini"'
Publikováno v:
Planta. 220:365-375
Two membrane-bound, ascorbate-dependent b-type cytochromes were identified in etiolated bean (Phaseolus vulgaris L.) hypocotyls. Following solubilization of microsomal membranes and anion-exchange chromatography at pH 8.0, two major cytochrome peaks
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7df3a7df205c7891c6724d2c2ad23632
https://doi.org/10.1007/s00425-004-1360-0
https://doi.org/10.1007/s00425-004-1360-0
Autor:
Francesca Sparla, Piera Sabatino, Simona Fermani, Paolo Trost, Giuseppe Zanotti, Sandra Scagliarini, Alberto Ripamonti, Paolo Pupillo
Publikováno v:
Journal of Molecular Biology. 314:527-542
Here, we report the first crystal structure of a photosynthetic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) complexed with NADP. The enzyme, purified from spinach chloroplasts, is constituted of a single type of subunit (A) arranged in homotetra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8cebf688dd6a3fbff350475888e340c2
https://doi.org/10.1006/jmbi.2001.5172
https://doi.org/10.1006/jmbi.2001.5172
Publikováno v:
Journal of Experimental Botany. 49:1307-1315
except that V max with NADP(H) was 8-fold lower on a protein basis. The kinetic data are consistent with a Isoforms of NAD(P)-dependent glyceraldehyde-3- GAPDH
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f55908c4ee96dcff73be6b93bcd4e6e0
https://doi.org/10.1093/jxb/49.325.1307
https://doi.org/10.1093/jxb/49.325.1307
Publikováno v:
Protoplasma. 205:66-73
The plasma membrane (PM) of higher plants contains a major ascorbate-reducible, high-potentialb-type cytochrome, named cytochromeb561 (cytb561). In this paper a rapid purification protocol for the cytb561 of bean hypocotyls PM is described. An almost
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a15ff765c548eb603b4a6cb0a5cbf4b1
https://doi.org/10.1007/bf01279295
https://doi.org/10.1007/bf01279295
Publikováno v:
European Journal of Biochemistry. 234:452-458
NAD(P)H :(quinone-acceptor) oxidoreductase [NAT)(P)H-QR], a plant cytosolic protein, was purified froni cultured sugarbeet cells by a combination of ainnlonium sulfatc fractionation, FPLC Superdex 200 gel filtration, Q-Sepharosc anion-cxchange chroma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6802c12d9f42c3a2be32dbe7d3ce7bc
https://doi.org/10.1111/j.1432-1033.1995.452_b.x
https://doi.org/10.1111/j.1432-1033.1995.452_b.x
Publikováno v:
FEBS Letters. 339:97-100
Non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.9) from spinach leaves was purified to homogeneity using an improved purification procedure. Thus, a major contaminant with molecular mass and ion-exchange
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be0207b695039f96577aec80e0ca9a3b
https://doi.org/10.1016/0014-5793(94)80393-5
https://doi.org/10.1016/0014-5793(94)80393-5
Publikováno v:
Plant Physiology. 94:1337-1344
The steady state kinetics of glyceraldehyde 3-phosphate:NADP + oxidoreductase (GNR) (EC 1.2.1.9) have been investigated. The enzyme exhibits hyperbolic behavior over a wide range of substrate concentrations. Double-reciprocal plots are nearly paralle
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3b5d1d71bf3e59763384c12f39572be
https://doi.org/10.1104/pp.94.3.1337
https://doi.org/10.1104/pp.94.3.1337
Autor:
Francesca Sparla, Sandra Scagliarini, Giuseppe Falini, Lucia Marri, Simona Fermani, Paolo Trost, Paolo Pupillo, Mirko Zaffagnini
Regulation of the Calvin-Benson cycle under varying light/dark conditions is a common property of oxygenic photosynthetic organisms and photosynthetic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the targets of this complex regulatory s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15c8cdc395dfd4660ffd4854d513fc02
http://hdl.handle.net/11585/32814
http://hdl.handle.net/11585/32814
Publikováno v:
Planta. 190
Spinach (Spinacia oleracea L.) chloroplast NAD(P)-dependent glyceraldehyde 3-phosphate dehydrogenase (NAD(P)-GAPDH; EC 1.2.1.13) was purified. The association state of the protein was monitored by fast protein liquid chromatography-Superose 12 gel fi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5b81e964500a8d8bbda3c7acc5ed341d
https://doi.org/10.1007/bf00196960
https://doi.org/10.1007/bf00196960
Publikováno v:
Planta. 190
Light modulation of chloroplast glyceraldehyde 3-phosphate dehydrogenase (NAD(P)-GAPDH; EC 1.2.1.13) has been investigated. Complete activation of NADPH-dependent activity is achieved at 25 W.m−2 photosynthetically active radiation in spinach (Spin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::90d481f799383ddf68e8f77e80342457
https://doi.org/10.1007/bf00196959
https://doi.org/10.1007/bf00196959