Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Sandra E. D'Souza"'
Publikováno v:
Journal of Environmental Pathology, Toxicology and Oncology. 27:181-189
A simple, single-step, extrusion-free protocol for preparing doxorubicin-loaded liposomes (100150 nm), based on the ethanol injection method (EIM), is described. Efficient encapsulation of doxorubicin (up to 98%) was obtained concomitantly with lipos
Publikováno v:
World Journal of Microbiology and Biotechnology. 13:561-564
Fructose-1,6-bisphosphate (FBP) aldolase (EC 4.1.2.13) of Haloferax mediterranei was immobilized by treating the cell extract in the presence of 10% BSA, with the cross-linking reagent, 0.5% glutaraldehyde for 15min, with the retention of 60% of its
Publikováno v:
Journal of Biochemical and Biophysical Methods. 24:239-247
The cells of Haloferax mediterannei were stabilized by cross-linking with 0.5% glutaraldehyde for 10 min. Such cells were found to be osmotically stable even when suspended in water. The stabilized cells could be permeabilized by treatment with chlor
Autor:
Wijaya Altekar, Sandra E. D'Souza
Publikováno v:
The Journal of general and applied microbiology. 44(4)
Fructose-1,6-bisphosphate (FBP) aldolase (EC 4.1.2.13) was purified 97-fold from a halophilic archaebacterium Haloferax mediterranei, with a specific activity of 2.8. The enzyme was characterized as a Class II aldolase on the basis of its inhibition
Adaptive response of Haloferax mediterranei to low concentrations of NaCl (20%) in the growth medium
Publikováno v:
Archives of microbiology. 168(1)
Halobacteria require 20-25% NaCl for optimal growth and lyse when the salt concentration falls below 10%. The response of Haloferax mediterranei cells to low concentrations of NaCl (< 20%) in the medium was studied. The cells adapted to and grew in c