Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Sandra A. Helquist"'
Publikováno v:
Journal of Biological Chemistry. 283:14402-14410
To assess the contribution to discrimination afforded by base pair hydrogen bonding during DNA replication by the human mitochondrial DNA polymerase, we examined nucleoside mimics lacking hydrogen bond forming capability but retaining the overall ste
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a086a7d1844cb52c65673d2cadb065b
https://doi.org/10.1074/jbc.m705007200
https://doi.org/10.1074/jbc.m705007200
Publikováno v:
Journal of Biological Chemistry. 283:14411-14416
We have characterized the role of Watson-Crick hydrogen bonding in the 3'-terminal base pair on the 3'-5' exonuclease activity of the human mitochondrial DNA polymerase. Nonpolar nucleoside analogs of thymidine (dF) and deoxyadenosine (dQ) were used
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51bdabee2aabd62acb968adf569d9ab7
https://doi.org/10.1074/jbc.m705006200
https://doi.org/10.1074/jbc.m705006200
Publikováno v:
Biochemistry. 45:2836-2844
Formation of a noncanonical base pair between dFTP, a dTTP analogue that cannot form H bonds, and the fluorescent base analogue 2-aminopurine (2AP) was studied in order to discover how the bacteriophage T4 DNA polymerase selects nucleotides with high
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::743b84332adb4a66075d80094bdb5a34
https://doi.org/10.1021/bi051644s
https://doi.org/10.1021/bi051644s
Autor:
Angela M. DeLucia, Nigel D. F. Grindley, Sandra A. Helquist, Chikio Chan, Catherine M. Joyce, Olga Potapova, Eric T. Kool
Publikováno v:
Biochemistry. 45:890-898
We report the first pre-steady-state kinetic studies of DNA replication in the absence of hydrogen bonds. We have used nonpolar nucleotide analogues that mimic the shape of a Watson-Crick base pair to investigate the kinetic consequences of a lack of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3aa650652cdd5265ec733341707004e5
https://doi.org/10.1021/bi051792i
https://doi.org/10.1021/bi051792i
Autor:
William T. Wolfle, Sandra A. Helquist, Louise Prakash, Eric T. Kool, M. Todd Washington, Thomas E. Spratt, Satya Prakash
Publikováno v:
Molecular and Cellular Biology. 25:7137-7143
The efficiency and fidelity of nucleotide incorporation by high-fidelity replicative DNA polymerases (Pols) are governed by the geometric constraints imposed upon the nascent base pair by the active site. Consequently, these polymerases can efficient
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f4dc74c0b14f9d2f6347e3ea8ce99e0
https://doi.org/10.1128/mcb.25.16.7137-7143.2005
https://doi.org/10.1128/mcb.25.16.7137-7143.2005
Publikováno v:
Journal of the American Chemical Society. 125:16235-16242
The Escherichia coli DNA repair enzymes Fpg and MutY are involved in the prevention of mutations resulting from 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG) in DNA. The nonpolar isosteres of 2'-deoxyadenosine, 4-methylbenzimidazole beta-deoxynucleoside (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e45bf842461c364095c556f35fa0925
https://doi.org/10.1021/ja0374426
https://doi.org/10.1021/ja0374426
Publikováno v:
Molecular and Cellular Biology. 23:5107-5112
Classical high-fidelity DNA polymerases discriminate between the correct and incorrect nucleotides by using geometric constraints imposed by the tight fit of the active site with the incipient base pair. Consequently, Watson-Crick (W-C) hydrogen bond
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5be7fdedb0ba73ec3f8a31580aa1e082
https://doi.org/10.1128/mcb.23.14.5107-5112.2003
https://doi.org/10.1128/mcb.23.14.5107-5112.2003
Autor:
Paul T. Henderson, Juan C. Morales, Eric T. Kool, John M. Essigmann, Sandra A. Helquist, James C. Delaney
Publikováno v:
Proceedings of the National Academy of Sciences. 100:4469-4473
We report studies testing the importance of Watson–Crick hydrogen bonding, base-pair geometry, and steric effects during DNA replication in living bacterial cells. Nonpolar DNA base shape mimics of thymine and adenine (abbreviated F and Q, respecti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::763e73aca5d8d890ac722619301e97cf
https://doi.org/10.1073/pnas.0837277100
https://doi.org/10.1073/pnas.0837277100
Autor:
Emin Oroudjev, Ravi A. Chandrasekaran, Gregory P. Miller, Lucian Orbai, Eric T. Kool, Helen G. Hansma, Sandra A. Helquist, Ulf M. Lindström
Publikováno v:
Proceedings of the National Academy of Sciences. 99:15953-15958
Human telomerase is a reverse-transcriptase enzyme that synthesizes the multikilobase repeating hexamer telomere sequence (TTAGGG) n at the ends of chromosomes. Here we describe a designed approach to mimicry of telomerase, in which synthetic DNA nan
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b52e6714b6b4819b80f0692243a41e36
https://doi.org/10.1073/pnas.252396199
https://doi.org/10.1073/pnas.252396199
Autor:
Eric T. Kool, Sandra A. Helquist, Bryan O'neill, Kristi L. Good, Juan C. Morales, Jessica E. Ratto, Deborah C. Tahmassebi
Publikováno v:
The Journal of Organic Chemistry. 67:5869-5875
We describe the preparation and structure of the deoxyribonucleoside of 4-fluoro-6-methylbenzimidazole, abbreviated dH (8), which acts as a close shape mimic of the nucleoside deoxyguanosine. The nucleoside is prepared from 2-fluoro-4-methylaniline i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eadb85c04c560fe7247cf478afa32103
https://doi.org/10.1021/jo025884e
https://doi.org/10.1021/jo025884e