Výsledky vyhledávání - "Sandra A Rutherford"

A protocol for isolation and visualization of yeast nuclei by scanning electron microscopy (SEM)

Autor: K. N. Morozova, Elena Kiseleva, Fiona Gardiner, Terence D Allen, Martin W. Goldberg, Sandra A Rutherford, Stephen M Murray, Sheona P Drummond

Publikováno v: Nature Protocols. 2:1943-1953

This protocol details methods for the isolation of yeast nuclei from budding yeast (Saccharomyces cerevisiae) and fission yeast (Schizosaccharomyces pombe), immuno-gold labeling of proteins and visualization by field emission scanning electron micros

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9065eb678df4125afa7180933158f4b3
https://doi.org/10.1038/nprot.2007.251

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A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM)

Autor: Fiona Gardiner, Terence D Allen, Helen S Sanderson, Martin W. Goldberg, Sandra A Rutherford, Stephen M Murray, Sheona P Drummond, Elena Kiseleva

Publikováno v: Nature Protocols. 2:1166-1172

This protocol details methods for the isolation of oocyte nuclear envelopes (NEs) from the African clawed toad Xenopus laevis, immunogold labeling of component proteins and subsequent visualization by field-emission scanning electron microscopy (FESE

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f5b288a6ba4889c3dd12a1693649573
https://doi.org/10.1038/nprot.2007.137

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Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro–formed nuclei for Western blotting and scanning electron microscopy (SEM)

Autor: Sheona P Drummond, Terence D Allen, Sandra A Rutherford, Stephen M Murray, Martin W. Goldberg, Fiona Gardiner, Elena Kiseleva, Helen S Sanderson

Publikováno v: Nature Protocols. 2:1173-1179

This protocol details methods for the generation of cell-free extracts and DNA templates from the eggs and sperm chromatin, respectively, of the clawed toad Xenopus laevis. We have used this system with scanning electron microscopy (SEM), as detailed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aeca550488d33b917295640361d567c4
https://doi.org/10.1038/nprot.2007.138

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Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells

Autor: Sheona P Drummond, Terence D Allen, Sandra A Rutherford, Stephen M Murray, Fiona Gardiner, Martin W. Goldberg, Elena Kiseleva

Publikováno v: Nature Protocols. 2:1180-1184

Our previous work characterizing the biogenesis and structural integrity of the nuclear envelope and nuclear pore complexes (NPCs) has been based on amphibian material but has recently progressed into the analysis of tissue-culture cells. This protoc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85bf3bc3aea9ab97c7e61fdc06e355ab
https://doi.org/10.1038/nprot.2007.139

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High resolution analysis of mammalian nuclear structure throughout the cell cycle: implications for nuclear pore complex assembly during interphase and mitosisThis paper is one of a selection of papers published in this Special Issue, entitled The Nucleus: A Cell Within A Cell

Autor: Helen S Sanderson, Terry Allen, Sheona P Drummond, Sandra A Rutherford

Publikováno v: Canadian Journal of Physiology and Pharmacology. 84:423-430

Nuclear pore complexes (NPCs) are the gateways for both active and passive bidirectional molecular transport between the nucleoplasm and cytoplasm. These mega-dalton assemblies are composed of multiple copies of approximately 30 distinct proteins ter

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::758bc204935b7edbd6413faaf0d9cc72
https://doi.org/10.1139/y05-148

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Actin- and protein-4.1-containing filaments link nuclear pore complexes to subnuclear organelles inXenopusoocyte nuclei

Autor: Sheona P Drummond, Katherine L. Wilson, Sandra A Rutherford, Elena Kiseleva, Martin W. Goldberg, Terence D Allen

Publikováno v: Journal of Cell Science. 117:2481-2490

We imaged the interiors of relatively intact Xenopus oocyte nuclei by field emission scanning electron microscopy (feSEM) and visualized a network of filaments that attach to nuclear pore complexes and extend throughout the nucleus. Within the nucleu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51c7f70c8eb06730233ca993c77808ba
https://doi.org/10.1242/jcs.01098

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Ran alters nuclear pore complex conformation

Autor: Laura A Cotter, Steven Bagley, Paul Clarke, Elena Kiseleva, Martin W. Goldberg, Mike F. Hughes, Terence D Allen, Sandra A Rutherford

Publikováno v: Journal of Molecular Biology. 300:519-529

Transport across the nuclear membranes occurs through the nuclear pore complex (NPC), and is mediated by soluble transport factors including Ran, a small GTPase that is generally GDP-bound during import and GTP-bound for export. The dynamic nature of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7786118694f89530ff3b30a9ffc06a2b
https://doi.org/10.1006/jmbi.2000.3891

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Macromolecular substructure in nuclear pore complexes by in-lens field-emission scanning electron microscopy

Autor: Elena Kiseleva, Siegfried Reipert, Gordon Bennion, Martin W. Goldberg, Sandra A Rutherford, A Ramalho, Terence D Allen

Publikováno v: Scanning. 19:403-410

Scanning electron microscopy (SEM) has produced a wealth of novel images that have significantly complemented our perception of biological structure and function, derived initially from transmission electron microscopy (TEM) information. SEM is a sur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e24b5fab710828c6d927188b7cbd8107
https://doi.org/10.1002/sca.4950190603

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Scanning electron microscopy of nuclear structure

Autor: Terence D, Allen, Sandra A, Rutherford, Stephen, Murray, Sheona P, Drummond, Martin W, Goldberg, Elena, Kiseleva

Publikováno v: Methods in cell biology. 88

Accessing internal structure and retaining relative three dimensional (3D) organization within the nucleus has always proved difficult in the electron microscope. This is due to the overall size and largely fibrous nature of the contents, making larg

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::b9ad86c86f479049350394fffecad517
https://pubmed.ncbi.nlm.nih.gov/18617044

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Chapter 20 Scanning Electron Microscopy of Nuclear Structure

Autor: Martin W. Goldberg, Elena Kiseleva, Sandra A Rutherford, Stephen M Murray, Sheona P Drummond, Terence D Allen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bbac7d971018fe6ce711d257bc91e874
https://doi.org/10.1016/s0091-679x(08)00420-2

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