A kinetic model predicts SpCas9 activity, improves off-target classification, and reveals the physical basis of targeting fidelity.

Autor: Eslami-Mossallam B; Kavli Institute of NanoScience and Department of BionanoScience, Delft University of Technology, Delft, 2629HZ, the Netherlands.; Dept. Building Physics and Systems, TNO Building and Construction Research, Leeghwaterstraat 44, Delft, The Netherlands., Klein M; Kavli Institute of NanoScience and Department of BionanoScience, Delft University of Technology, Delft, 2629HZ, the Netherlands.; Department of Physics and Astronomy, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam, De Boelelaan 1081, 1081 HV, Amsterdam, the Netherlands., Smagt CVD; Kavli Institute of NanoScience and Department of BionanoScience, Delft University of Technology, Delft, 2629HZ, the Netherlands.; Department of Physics and Astronomy, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam, De Boelelaan 1081, 1081 HV, Amsterdam, the Netherlands., Sanden KVD; Kavli Institute of NanoScience and Department of BionanoScience, Delft University of Technology, Delft, 2629HZ, the Netherlands., Jones SK Jr; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, 78712, USA.; Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, 78712, USA.; Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX, 78712, USA.; VU LSC-EMBL Partnership for Genome Editing Technologies, Life Sciences Center, Vilnius University, Vilnius, Lithuania., Hawkins JA; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, 78712, USA.; Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, 78712, USA.; Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX, 78712, USA.; Oden Institute for Computational Engineering and Science, University of Texas at Austin, Austin, TX, 78712, USA.; European Molecular Biology Laboratory, Genome Biology Department, Heidelberg, Germany., Finkelstein IJ; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, 78712, USA.; Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, 78712, USA.; Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX, 78712, USA., Depken M; Kavli Institute of NanoScience and Department of BionanoScience, Delft University of Technology, Delft, 2629HZ, the Netherlands. S.M.Depken@tudelft.nl.

Publikováno v: Nature communications [Nat Commun] 2022 Mar 15; Vol. 13 (1), pp. 1367. Date of Electronic Publication: 2022 Mar 15.