Zobrazeno 1 - 10
of 64
pro vyhledávání: '"Sanchita Bhadra"'
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-11 (2023)
Abstract Lyme disease, one of the most common tickborne diseases, has been rapidly spreading in parallel with the expansion of the range of its tick vector. Better tick surveillance efforts are needed to accurately estimate disease risk and to guide
Externí odkaz:
https://doaj.org/article/0c6f597641814631849b9f7f014024de
Publikováno v:
PLoS ONE, Vol 17, Iss 5, p e0268575 (2022)
The ability to predict nucleic acid hybridization energies has been greatly enabling for many applications, but predictive models require painstaking experimentation, which may limit expansion to non-natural nucleic acid analogues and chemistries. We
Externí odkaz:
https://doaj.org/article/df1a2278060a4efc88d97cf6fe785aab
Publikováno v:
mSphere, Vol 6, Iss 3 (2021)
One of the key approaches to treatment and control of infectious diseases, such as COVID-19, is accurate and rapid diagnostics that is widely deployable in a timely and scalable manner. To achieve this, it is essential to go beyond the traditional go
Externí odkaz:
https://doaj.org/article/ab996ce1cb4e43d5bf31f1c6c377013f
Publikováno v:
Bio-Protocol, Vol 11, Iss 2 (2021)
Given the scale of the ongoing COVID-19 pandemic, the need for reliable, scalable testing, and the likelihood of reagent shortages, especially in resource-poor settings, we have developed an RT-qPCR assay that relies on an alternative to conventional
Externí odkaz:
https://doaj.org/article/48fbaacba7fc4821ae4266cd1f9c4061
Autor:
Sanchita Bhadra, Vylan Nguyen, Jose-Angel Torres, Shaunak Kar, Stéphane Fadanka, Chiara Gandini, Harry Akligoh, Inyup Paik, Andre C Maranhao, Jenny Molloy, Andrew D Ellington
Publikováno v:
PLoS ONE, Vol 16, Iss 6, p e0252507 (2021)
We recently developed 'cellular' reagents-lyophilized bacteria overexpressing proteins of interest-that can replace commercial pure enzymes in typical diagnostic and molecular biology reactions. To make cellular reagent technology widely accessible a
Externí odkaz:
https://doaj.org/article/e35fa46120fb4ebfae7c4478329c5534
Autor:
Sanchita Bhadra, Timothy E Riedel, Miguel A Saldaña, Shivanand Hegde, Nicole Pederson, Grant L Hughes, Andrew D Ellington
Publikováno v:
PLoS Neglected Tropical Diseases, Vol 12, Iss 8, p e0006671 (2018)
Manipulation of natural mosquito populations using the endosymbiotic bacteria Wolbachia is being investigated as a novel strategy to reduce the burden of mosquito-borne viruses. To evaluate the efficacy of these interventions, it will be critical to
Externí odkaz:
https://doaj.org/article/5113a60a2cf3435bab15aa018b5c15b2
Autor:
Sanchita Bhadra, Arti Pothukuchy, Raghav Shroff, Austin W Cole, Michelle Byrom, Jared W Ellefson, Jimmy D Gollihar, Andrew D Ellington
Publikováno v:
PLoS ONE, Vol 13, Iss 8, p e0201681 (2018)
We have found that the overproduction of enzymes in bacteria followed by their lyophilization leads to 'cellular reagents' that can be directly used to carry out numerous molecular biology reactions. We demonstrate the use of cellular reagents in a v
Externí odkaz:
https://doaj.org/article/c8cea68c2ab242859670f4198d96c323
Publikováno v:
Viruses, Vol 10, Iss 12, p 714 (2018)
We have developed a generalizable “smart molecular diagnostic„ capable of accurate point-of-care (POC) detection of variable nucleic acid targets. Our isothermal assay relies on multiplex execution of four loop-mediated isothermal amplification r
Externí odkaz:
https://doaj.org/article/bcc24496b6fc45a2aef384c58adf55ac
Autor:
Sanchita Bhadra, Yu Sherry Jiang, Mia R Kumar, Reed F Johnson, Lisa E Hensley, Andrew D Ellington
Publikováno v:
PLoS ONE, Vol 10, Iss 4, p e0123126 (2015)
The Middle East respiratory syndrome coronavirus (MERS-CoV), an emerging human coronavirus, causes severe acute respiratory illness with a 35% mortality rate. In light of the recent surge in reported infections we have developed asymmetric five-prime
Externí odkaz:
https://doaj.org/article/23515e760c174d4887d51de0e16481b5
Publikováno v:
PLoS Pathogens, Vol 2, Iss 12, p e128 (2006)
Most inbred mice carry germline proviruses of the retrovirus, mouse mammary tumor virus (MMTV) (called Mtvs), which have multiple replication defects. A BALB/c congenic mouse strain lacking all endogenous Mtvs (Mtv-null) was resistant to MMTV oral an
Externí odkaz:
https://doaj.org/article/ac5e75d6f5214270ac2391acc2753d64