Zobrazeno 1 - 10
of 64
pro vyhledávání: '"Sanchita, Bhadra"'
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-11 (2023)
Abstract Lyme disease, one of the most common tickborne diseases, has been rapidly spreading in parallel with the expansion of the range of its tick vector. Better tick surveillance efforts are needed to accurately estimate disease risk and to guide
Externí odkaz:
https://doaj.org/article/0c6f597641814631849b9f7f014024de
Publikováno v:
PLoS ONE, Vol 17, Iss 5, p e0268575 (2022)
The ability to predict nucleic acid hybridization energies has been greatly enabling for many applications, but predictive models require painstaking experimentation, which may limit expansion to non-natural nucleic acid analogues and chemistries. We
Externí odkaz:
https://doaj.org/article/df1a2278060a4efc88d97cf6fe785aab
Publikováno v:
mSphere, Vol 6, Iss 3 (2021)
One of the key approaches to treatment and control of infectious diseases, such as COVID-19, is accurate and rapid diagnostics that is widely deployable in a timely and scalable manner. To achieve this, it is essential to go beyond the traditional go
Externí odkaz:
https://doaj.org/article/ab996ce1cb4e43d5bf31f1c6c377013f
Publikováno v:
Bio-Protocol, Vol 11, Iss 2 (2021)
Given the scale of the ongoing COVID-19 pandemic, the need for reliable, scalable testing, and the likelihood of reagent shortages, especially in resource-poor settings, we have developed an RT-qPCR assay that relies on an alternative to conventional
Externí odkaz:
https://doaj.org/article/48fbaacba7fc4821ae4266cd1f9c4061
Autor:
Sanchita Bhadra, Vylan Nguyen, Jose-Angel Torres, Shaunak Kar, Stéphane Fadanka, Chiara Gandini, Harry Akligoh, Inyup Paik, Andre C Maranhao, Jenny Molloy, Andrew D Ellington
Publikováno v:
PLoS ONE, Vol 16, Iss 6, p e0252507 (2021)
We recently developed 'cellular' reagents-lyophilized bacteria overexpressing proteins of interest-that can replace commercial pure enzymes in typical diagnostic and molecular biology reactions. To make cellular reagent technology widely accessible a
Externí odkaz:
https://doaj.org/article/e35fa46120fb4ebfae7c4478329c5534
Publikováno v:
Nucleic Acids Research. 51:488-499
Loop-mediated isothermal amplification (LAMP) has proven to be easier to implement than PCR for point-of-care diagnostic tests. However, the underlying mechanism of LAMP is complicated and the kinetics of the major steps in LAMP have not been fully e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bd790ce348cc216da9ab296dcccb670
https://doi.org/10.1093/nar/gkac1221
https://doi.org/10.1093/nar/gkac1221
Autor:
Inyup, Paik, Phuoc H T, Ngo, Raghav, Shroff, Daniel J, Diaz, Andre C, Maranhao, David J F, Walker, Sanchita, Bhadra, Andrew D, Ellington
Publikováno v:
Biochemistry
The DNA polymerase I from Geobacillus stearothermophilus (also known as Bst DNAP) is widely used in isothermal amplification reactions, where its strand displacement ability is prized. More robust versions of this enzyme should be enabled for diagnos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8764414f6e516fa3e1091f62c4301641
https://pubmed.ncbi.nlm.nih.gov/34762799
https://pubmed.ncbi.nlm.nih.gov/34762799
Autor:
Sanchita Bhadra, Timothy E Riedel, Miguel A Saldaña, Shivanand Hegde, Nicole Pederson, Grant L Hughes, Andrew D Ellington
Publikováno v:
PLoS Neglected Tropical Diseases, Vol 12, Iss 8, p e0006671 (2018)
Manipulation of natural mosquito populations using the endosymbiotic bacteria Wolbachia is being investigated as a novel strategy to reduce the burden of mosquito-borne viruses. To evaluate the efficacy of these interventions, it will be critical to
Externí odkaz:
https://doaj.org/article/5113a60a2cf3435bab15aa018b5c15b2
Autor:
Sanchita Bhadra, Arti Pothukuchy, Raghav Shroff, Austin W Cole, Michelle Byrom, Jared W Ellefson, Jimmy D Gollihar, Andrew D Ellington
Publikováno v:
PLoS ONE, Vol 13, Iss 8, p e0201681 (2018)
We have found that the overproduction of enzymes in bacteria followed by their lyophilization leads to 'cellular reagents' that can be directly used to carry out numerous molecular biology reactions. We demonstrate the use of cellular reagents in a v
Externí odkaz:
https://doaj.org/article/c8cea68c2ab242859670f4198d96c323
Autor:
Raghav Shroff, Phuoc H. T. Ngo, Daniel J. Diaz, Sanchita Bhadra, Andrew D. Ellington, Andre C. Maranhao, Inyup Paik, David J. F. Walker
Publikováno v:
Biochemistry. 62:410-418
The DNA polymerase I from Geobacillus stearothermophilus (also known as Bst DNAP) is widely used in isothermal amplification reactions, where its strand displacement ability is prized. More robust versions of this enzyme should be enabled for diagnos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ed3c1631add22c8a621d58b8a3ccdc9f
https://doi.org/10.1021/acs.biochem.1c00451
https://doi.org/10.1021/acs.biochem.1c00451