Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Samy Majoul"'
Publikováno v:
Biochemical Engineering Journal. 90:131-139
Sheep pox virus initially adapted to replicate in primary lamb kidney cells was adapted to Vero cells by serial passages in monolayer cultures. After nine passages the virus was able to correctly replicate in Vero cells, virus titer achieved was 10 5
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cea975030bf40377e30aeb368c20da43
https://doi.org/10.1016/j.bej.2014.06.001
https://doi.org/10.1016/j.bej.2014.06.001
Publikováno v:
Applied Biochemistry and Biotechnology. 170:1724-1737
Subcultivation of Vero cells grown in a proprietary animal component-free medium named IPT-AFM, on microcarriers, was studied. TrypLE Select, a non-animal-derived protease, was used as an alternative to trypsin for cell passaging. We first studied th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::320d7e071d56e35b4e934305f650c8ff
https://doi.org/10.1007/s12010-013-0307-y
https://doi.org/10.1007/s12010-013-0307-y
Publikováno v:
Applied Microbiology and Biotechnology. 93:1031-1040
Measles vaccination remains the most efficient way to control the spread of the virus. This work focuses on the production of a measles vaccine using stirred conditions as an advanced option for process scale up. Non-porous Cytodex 1 microcarriers we
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26c1354bf5bb0e662caf7bb09c83a8e9
https://doi.org/10.1007/s00253-011-3574-y
https://doi.org/10.1007/s00253-011-3574-y
Autor:
Héla Kallel, Arno van der Ark, Samia Rourou, Samy Majoul, Khaled Trabelsi, Tiny van der Velden
Publikováno v:
Applied Microbiology and Biotechnology. 85:53-63
Vero cells growth and rabies production in IPT-AF medium, a property animal-component-free medium are described in this work. Kinetics of cell growth and rabies virus (strain LP 2061) production were first conducted in spinner flasks. Over eight inde
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cddddee6b86e57324a21414509c1c44b
https://doi.org/10.1007/s00253-009-2064-y
https://doi.org/10.1007/s00253-009-2064-y
Publikováno v:
Enzyme and Microbial Technology. 36:514-519
We studied Vero cell growth on Cytodex 1 (3 g/l) and MEM supplemented with foetal calf serum (FCS) in a 2-l bioreactor using continuous (recirculation and perfusion) and batch culture modes. We achieved a cell density level equal to 4.35 × 10 6 cell
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b04c35100bb77fb9b17952751e9c504d
https://doi.org/10.1016/j.enzmictec.2004.11.008
https://doi.org/10.1016/j.enzmictec.2004.11.008
Publikováno v:
Applied Microbiology and Biotechnology. 61:441-446
We studied BHK-21 cells growth in a 2-l bioreactor and investigated the effects of microcarrier concentration, type of growth medium, culture mode and serum concentration. The highest cell density reached was equal to 4x10(6) cells/ml and was achieve
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f774dbf36943bc51f7d38a6fbfcb0bda
https://doi.org/10.1007/s00253-003-1245-3
https://doi.org/10.1007/s00253-003-1245-3
Publikováno v:
Vaccine. 32(24)
IPT-AFM is a proprietary animal component free medium that was developed for rabies virus (strain LP 2061) production in Vero cells. In the present work, we demonstrated the versatility of this medium and its ability to sustain the growth of other ce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::37565e52feac49f4264a5f35693bff17
https://pubmed.ncbi.nlm.nih.gov/24583007
https://pubmed.ncbi.nlm.nih.gov/24583007
Autor:
Erdmann Rapp, Dirk Benndorf, D. Vester, Samia Rourou, Héla Kallel, Sabine Kluge, Samy Majoul, Udo Reichl, Yvonne Genzel
Publikováno v:
Applied microbiology and biotechnology. 97(12)
The use of Vero cells for rabies vaccine production was recommended from the WHO in 2005. A controlled production process is necessary to reduce the risk of contaminants in the product. One step towards this is to turn away from animal-derived compon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7b3861c2e275833385a8ab9cf1e2fc3
https://pubmed.ncbi.nlm.nih.gov/23674149
https://pubmed.ncbi.nlm.nih.gov/23674149
Publikováno v:
Animal Cell Technology: Basic & Applied Aspects ISBN: 9789048165575
We studied BHK-21 cells growth in a 2 liter bioreactor and investigated the effects of microcarriers concentration, nature of growth medium, culture mode and serum concentration. We have reached a cell density of 4×106 cells/ml under the following g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b8d4513300364ab02fcb524ddf1f30df
https://doi.org/10.1007/978-94-017-0726-8_43
https://doi.org/10.1007/978-94-017-0726-8_43
Publikováno v:
Journal of biotechnology. 95(3)
We have carried out the adaptation of BHK-21 cells to two serum free (Ex Cell 520 and HyQ PF CHO) and three animal protein free media: Ex Cell 302, HyQ PF CHO MPS and Rencyte BHK. After a direct switch or a gradual adaptation, we have achieved BHK-21
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9863f7330f8ab1327906c71268f40945
https://pubmed.ncbi.nlm.nih.gov/12007860
https://pubmed.ncbi.nlm.nih.gov/12007860