Sensing Enzyme Activation Heat Capacity at the Single-Molecule Level Using Gold-Nanorod-Based Optical Whispering Gallery Modes

Autor: Vickery L. Arcus, Sivaraman Subramanian, Hannah B. L. Jones, Marc W. van der Kamp, Christopher R. Pudney, Frank Vollmer, Samuel Winter, Simona Frustaci

Publikováno v: et al. 2021, ' Sensing Enzyme Activation Heat Capacity at the Single-Molecule Level Using Gold-Nanorod-Based Optical Whispering Gallery Modes ', ACS Applied Nano Materials, vol. 4, no. 5 . https://doi.org/10.1021/acsanm.1c00176
ACS Applied Nano Materials

Here, we report a label-free gold nanoparticle-based single-molecule optical platform to study the immobilization, activity, and thermodynamics of single enzymes. The sensor uses plasmonic gold nanoparticles coupled to optical whispering gallery mode

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12f249d84cbfce55f90a194c67afcb66
https://doi.org/10.1021/acsanm.1c00176

Chemical Mapping Exposes the Importance of Active Site Interactions in Governing the Temperature Dependence of Enzyme Turnover

Autor: Vickery L. Arcus, Samuel Winter, Christopher R. Pudney, Erica J. Prentice, Dora M. Răsădean, Hannah B. L. Jones, Michael J. Danson, G. Dan Pantoş, Rory M. Crean, Gergely Katona, Marc W. van der Kamp

Publikováno v: Winter, S, Jones, H B L, Răsădean, D M, Crean, R M, Van der Kamp, M W & Pudney, C R 2021, ' Chemical Mapping Exposes the Importance of Active Site Interactions in Governing the Temperature Dependence of Enzyme Turnover ', ACS Catalysis, vol. 11, no. 24, pp. 14854-14863 . https://doi.org/10.1021/acscatal.1c04679
ACS Catalysis

Uncovering the role of global protein dynamics in enzyme turnover is needed to fully understand enzyme catalysis. Recently, we have demonstrated that the heat capacity of catalysis, ΔCP‡, can reveal links between the protein free energy landscape,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::190ba99b0f95305e563e8bbbec3a3647
https://research-information.bris.ac.uk/en/publications/5ae0ae7e-e25c-499a-8349-e605a8e52e02

Author response for 'Structure and in silico simulations of a cold-active esterase reveals its prime cold-adaptation mechanism'

Autor: Nehad Noby, Stephen A. Wells, D. Dafydd Jones, Ahmed Hussein, Husam Sabah Auhim, Hesham Saeed, Pierre J. Rizkallah, Samuel Winter, Christopher R. Pudney, Amira M. Embaby, Harley L. Worthy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::193da1ef5af00cabcda2f0d59fd2af21
https://doi.org/10.1098/rsob.210182/v2/response1

Text 3: Irish Ministers’ reply to the Worcestershire Association, 16 January 1655{/6}

Autor: Oliver Huchinson, William Markham, Samuel Winter

Publikováno v: Reliquiae Baxterianae: Or, Mr. Richard Baxter's Narrative of the Most Memorable Passages of his Life and Times

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::359c1159f698d290891f7df32b2c66c7
https://doi.org/10.1093/oseo/instance.00266733

Brain networks supporting execution of mathematical skills versus acquisition of new mathematical competence.

Autor: Samuel Wintermute, Shawn Betts, Jennifer L Ferris, Jon M Fincham, John R Anderson

Publikováno v: PLoS ONE, Vol 7, Iss 12, p e50154 (2012)

This fMRI study examines how students extend their mathematical competence. Students solved a set of algebra-like problems. These problems included Regular Problems that have a known solution technique and Exception Problems that but did not have a k

Externí odkaz: https://doaj.org/article/ffc5d6fa7ef246e9b4a52b183f870990