Heterologous production, reconstitution and EPR spectroscopic analysis of prFMN dependent enzymes

Autor: Huan-Ming Huang, David J. Procter, Godwin A. Aleku, Arune Balaikaite, Annica Saaret, Stephen E. J. Rigby, Irina Gostimskaya, Karl A. P. Payne, David Leys, Karl Fisher, Samuel S. Bailey, Stephen A. Marshall, Deepankar Gahloth

The recent discovery of the prenylated FMN (prFMN) cofactor has led to a renewed interest in the prFMN-dependent UbiD family of enzymes. The latter catalyses the reversible decarboxylation of alpha-beta unsaturated carboxylic acids and features widel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::228a1fb4edd16998ea10c817d65ecc15
https://doi.org/10.1016/bs.mie.2019.03.022

Terminal Alkenes from Acrylic Acid Derivatives via Non-Oxidative Enzymatic Decarboxylation by Ferulic Acid Decarboxylases

Autor: Godwin A. Aleku, Silvia M. Glueck, Karl A. P. Payne, Ruth T. Bradshaw-Allen, David A. Parker, Katharina Plasch, Samuel S. Bailey, Kurt Faber, Christoph Prause, David Leys

Publikováno v: ChemCatChem
Chemcatchem
Aleku, G A, Prause, C, Bradshaw-Allen, R T, Plasch, K, Glueck, S M, Bailey, S S, Payne, K A P, Parker, D A, Faber, K & Leys, D 2018, ' Terminal Alkenes from Acrylic Acid Derivatives via Non-Oxidative Enzymatic Decarboxylation by Ferulic Acid Decarboxylases ', ChemCatChem, vol. 10, no. 17, pp. 3736-3745 . https://doi.org/10.1002/cctc.201800643

Fungal ferulic acid decarboxylases (FDCs) belong to the UbiD-family of enzymes and catalyse the reversible (de)carboxylation of cinnamic acid derivatives through the use of a prenylated flavin cofactor. The latter is synthesised by the flavin prenylt

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d47da3d88bac37e5b51a070c1718f0cb
https://pubmed.ncbi.nlm.nih.gov/30333895

New cofactor supports α,β-unsaturated acid decarboxylation via 1,3-dipolar cycloaddition

Autor: Basile Khara, Sam Hay, Drupad K. Trivedi, Karl A. P. Payne, David Leys, Stephen E. J. Rigby, Perdita E. Barran, Mark D. White, Nigel S. Scrutton, Royston Goodacre, Rebecca Beveridge, David A. Parker, Samuel S. Bailey, Nicholas J. W. Rattray, Karl Fisher

Publikováno v: Nature. 522:497-501

The bacterial ubiD and ubiX or the homologous fungal fdc1 and pad1 genes have been implicated in the non-oxidative reversible decarboxylation of aromatic substrates, and play a pivotal role in bacterial ubiquinone (also known as coenzyme Q) biosynthe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::be3267a4fcdc2a86cf619e8fc4f0f065
https://doi.org/10.1038/nature14560

MelRisk: Using neutrophil-to-lymphocyte ratio to improve risk prediction models for metastatic cutaneous melanoma in the sentinel lymph node.

Autor: Wade RG; Faculty of Medicine and Health, Worsley Building, University of Leeds, Leeds, UK; Department of Plastic and Reconstructive Surgery, Leeds General Infirmary, Leeds, UK. Electronic address: ryckiewade@gmail.com., Bailey S; Department of Plastic and Reconstructive Surgery, Leeds General Infirmary, Leeds, UK., Robinson AV; Faculty of Medicine and Health, Worsley Building, University of Leeds, Leeds, UK., Lo MCI; Norwich Medical School, University of East Anglia, Norwich, UK., Peach H; Department of Plastic and Reconstructive Surgery, Leeds General Infirmary, Leeds, UK., Moncrieff MDS; Department of Plastic & Reconstructive Surgery, Norfolk & Norwich University Hospital NHS Trust, Norwich, UK; Norwich Medical School, University of East Anglia, Norwich, UK., Martin J; Institute of Applied Health Research, University of Birmingham, Birmingham, UK.

Publikováno v: Journal of plastic, reconstructive & aesthetic surgery : JPRAS [J Plast Reconstr Aesthet Surg] 2022 May; Vol. 75 (5), pp. 1653-1660. Date of Electronic Publication: 2021 Dec 01.

The challenge of comprehensive and consistent sequence variant interpretation between clinical laboratories.

Autor: Pepin MG; Department of Pathology, University of Washington, Seattle, Washington, USA., Murray ML; Department of Pathology, University of Washington, Seattle, Washington, USA.; Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, Washington, USA., Bailey S; Department of Pathology, University of Washington, Seattle, Washington, USA., Leistritz-Kessler D; Department of Pathology, University of Washington, Seattle, Washington, USA., Schwarze U; Department of Pathology, University of Washington, Seattle, Washington, USA., Byers PH; Department of Pathology, University of Washington, Seattle, Washington, USA.; Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, Washington, USA.

Publikováno v: Genetics in medicine : official journal of the American College of Medical Genetics [Genet Med] 2016 Jan; Vol. 18 (1), pp. 20-4. Date of Electronic Publication: 2015 Apr 02.