Reporter gene-engineering of human induced pluripotent stem cells during differentiation renders in vivo traceable hepatocyte-like cells accessible

Autor: Candice Ashmore-Harris, Samuel JI Blackford, Benjamin Grimsdell, Ewelina Kurtys, Marlies C Glatz, Tamir S Rashid, Gilbert O Fruhwirth

Publikováno v: Stem Cell Research, Vol 41, Iss , Pp - (2019)

Primary hepatocyte transplantation (HTx) is a safe cell therapy for patients with liver disease, but wider application is circumvented by poor cell engraftment due to limitations in hepatocyte quality and transplantation strategies. Hepatocyte-like c

Externí odkaz: https://doaj.org/article/18160a1e0fba4e229b85aa66180b66fa

Reporter gene-engineering of human induced pluripotent stem cells during differentiation renders in vivo traceable hepatocyte-like cells accessible

Autor: Tamir S Rashid, Marlies C Glatz, Samuel Ji Blackford, Gilbert O. Fruhwirth, Ewelina Kurtys, Candice Ashmore-Harris, Benjamin Grimsdell

Publikováno v: Ashmore-Harris, C, Blackford, S JI, Grimsdell, B, Kurtys, E, Glatz, M, Rashid, T S & Fruhwirth, G O 2019, ' Reporter gene-engineering of human induced pluripotent stem cells during differentiation renders in vivo traceable hepatocyte-like cells accessible ', Stem Cell Research, vol. 41, 101599 . https://doi.org/10.1016/j.scr.2019.101599
Stem Cell Research
Stem Cell Research, Vol 41, Iss, Pp-(2019)

Highlights • iPSC-derived hepatocyte-like cells (HLCs) rendered traceable in vivo. • Reproducible lentivirus-based gene transfer during the differentiation process. • Protocol and reporter expression did not negatively impact on HLC maturation.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::547de406e39af55aad186afe5402f343
https://kclpure.kcl.ac.uk/en/publications/417d11b8-857c-4ba5-b2be-43b7fa7f78fb