Restoring the biological activity of crizanlizumab at physiological conditions through a pH-dependent aspartic acid isomerization reaction

Autor: Fabian Bickel, François Griaud, Wolfram Kern, Frieder Kroener, Manuela Gritsch, Jérôme Dayer, Samuel Barteau, Blandine Denefeld, Chi-Ya Kao-Scharf, Manuel Lang, Izabela Slupska-Muanza, Carla Schmidt, Matthias Berg, Jürgen Sigg, Lina Boado, Dirk Chelius

Publikováno v: mAbs, Vol 15, Iss 1 (2023)

ABSTRACTIn this study, we report the isomerization of an aspartic acid residue in the complementarity-determining region (CDR) of crizanlizumab as a major degradation pathway. The succinimide intermediate and iso-aspartic acid degradation products we

Externí odkaz: https://doaj.org/article/704ad1576cf945d7aab734d00191b085

Synthesis and in Vitro and in Vivo Pharmacological Evaluation of New 4-Aminoquinoline-Based Compounds

Autor: Kenneth J. Swart, Samuel Barteau, Jennifer Norman, Liezl Gibhard, Lubbe Wiesner, Efrem T. Abay, Philip J. Rosenthal, Jacques Kameni-Tcheudji, Jiri Gut, Matshawandile Tukulula, Mathew Njoroge, Dale Taylor, Kelly Chibale, Judith Streckfuss, Grace Mugumbate

Publikováno v: ACS medicinal chemistry letters, vol 4, iss 12

A new class of 4-aminoquinolines was synthesized and evaluated in vitro for antiplasmodial activity against both the chloroquine-sensitive (3D7) and -resistant (K1 and W2) strains. The most active compounds 3c-3e had acceptable cytotoxicity but showe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::018c8e18d0bb7117c3975ce8acda801d
https://doi.org/10.1021/ml400311r

The flexibility of a generic LC-MS/MS method for the quantitative analysis of therapeutic proteins based on human immunoglobulin G and related constructs in animal studies

Autor: Olivier Kretz, Thierry Wolf, Christian Lanshoeft, Markus Walles, Franck Picard, Olivier Heudi, Samuel Barteau, Sarah Cianférani

Publikováno v: Journal of Pharmaceutical and Biomedical Analysis
Journal of Pharmaceutical and Biomedical Analysis, Elsevier, 2016, 131, pp.214-222. ⟨10.1016/j.jpba.2016.08.039⟩

International audience; An increasing demand of new analytical methods is associated with the growing number of biotherapeutic programs being prosecuted in the pharmaceutical industry. Whilst immunoassay has been the standard method for decades, a gr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d319995649da569339d522fc67142539
https://pubmed.ncbi.nlm.nih.gov/27599352

Quantitative analysis of maytansinoid (DM1) in human serum by on-line solid phase extraction coupled with liquid chromatography tandem mass spectrometry - Method validation and its application to clinical samples

Autor: Olivier Kretz, Olivier Heudi, Samuel Barteau, Franck Picard

Publikováno v: Journal of pharmaceutical and biomedical analysis. 120

A sensitive and specific method was developed and validated for the quantitation of maytansinoid (DM1) in human serum using on-line solid phase extraction (SPE)-liquid chromatography-tandem mass spectrometry (LC-MS/MS). Because DM1 contains a free th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd3c9efd79630e4747d5572e06a5a67c
https://pubmed.ncbi.nlm.nih.gov/26771131

A sensitive LC-MS/MS method for quantification of a nucleoside analog in plasma: application to in vivo rat pharmacokinetic studies

Autor: Olivier Heudi, Samuel Barteau, Franck Picard, Olivier Kretz

Publikováno v: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 877(20-21)

A LC-MS/MS method was developed and validated for determination of nucleoside analog (NA) in rat plasma. The method run time was 6 min and the limit of quantification (LOQ) was estimated at 100 pg/mL. The extraction procedure consisted on plasma samp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e6b21f8392ada9bb31a44cb54fd80b1
https://pubmed.ncbi.nlm.nih.gov/19501028