Discovery of Small-Molecule CD33 Pre-mRNA Splicing Modulators

Autor: Thomas A. Chappie, Mario Abdelmessih, Claude W. Ambroise, Markus Boehm, Mi Cai, Michael Green, Edward Guilmette, Claire M. Steppan, Lucy M. Stevens, Liuqing Wei, Simon Xi, Samuel A. Hasson

Publikováno v: ACS Med Chem Lett

[Image: see text] CD33/Siglec 3 is a myeloid lineage cell surface receptor that is known to regulate microglia activity. Multiple genome-wide association studies (GWAS) have identified genetic variants in the CD33 gene that convey protection from lat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf6ae24644520418da58b2523e34f2ef
https://europepmc.org/articles/PMC8762744/

SRSF1 and PTBP1 Are trans-Acting Factors That Suppress the Formation of a CD33 Splicing Isoform Linked to Alzheimer’s Disease Risk

Autor: Uthpala Seneviratne, Robert V. Stanton, Samuel A. Hasson, Petra van Bergeijk, Estel Aparicio-Prat

Publikováno v: Molecular and Cellular Biology. 39

A single nucleotide polymorphism (SNP) in exon 2 of the CD33 gene is associated with reduced susceptibility to late-onset Alzheimer's disease (AD) and causal for elevated mRNA lacking exon 2. In contrast to full-length CD33, transcripts lacking exon

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c79e626d043607b8a05a020f1e9c68ee
https://doi.org/10.1128/mcb.00568-18

SRSF1 and PTBP1 Are

Autor: Petra, van Bergeijk, Uthpala, Seneviratne, Estel, Aparicio-Prat, Robert, Stanton, Samuel A, Hasson

Publikováno v: Molecular and Cellular Biology

A single nucleotide polymorphism (SNP) in exon 2 of the CD33 gene is associated with reduced susceptibility to late-onset Alzheimer’s disease (AD) and causal for elevated mRNA lacking exon 2. In contrast to full-length CD33, transcripts lacking exo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ec72fe15ec1a7810dbf008840d60ca71
https://pubmed.ncbi.nlm.nih.gov/31208978

Adaptation of a Genetic Screen Reveals an Inhibitor for Mitochondrial Protein Import Component Tim44

Autor: Zhiye Tang, Non Miyata, Colin J. Douglas, Carla M. Koehler, Chia-en A. Chang, Michael A. Conti, Robert Damoiseaux, Samuel A. Hasson, Meghan E. Johnson

Publikováno v: The Journal of biological chemistry, vol 292, iss 13
Miyata, N; Tang, Z; Conti, MA; Johnson, ME; Douglas, CJ; Hasson, SA; et al.(2017). Adaptation of a Genetic Screen Reveals an Inhibitor for Mitochondrial Protein Import Component Tim44. JOURNAL OF BIOLOGICAL CHEMISTRY, 292(13), 5429-5442. doi: 10.1074/jbc.M116.770131. UCLA: Retrieved from: http://www.escholarship.org/uc/item/7nc145n7

Diverse protein import pathways into mitochondria use translocons on the outer membrane (TOM) and inner membrane (TIM). We adapted a genetic screen, based on Ura3 mistargeting from mitochondria to the cytosol, to identify small molecules that attenua

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::748dfe86f91b9ba566231d16ac45d8bf
https://pubmed.ncbi.nlm.nih.gov/28167535

Application of Imaging-Based Assays in Microplate Formats for High-Content Screening

Autor: Adam I. Fogel, Scott E. Martin, Samuel A. Hasson

Publikováno v: Methods in Molecular Biology ISBN: 9781493936717

The use of multiparametric microscopy-based screens with automated analysis has enabled the large-scale study of biological phenomena that are currently not measurable by any other method. Collectively referred to as high-content screening (HCS), or

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6b8ada65ebe3e2fe21df6ab639ce613d
https://doi.org/10.1007/978-1-4939-3673-1_18