Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Sampada Kalan"'
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Cdk9 (P-TEFb) and its substrate Spt5 influence events throughout the transcription cycle. Here, the authors define two switches with the potential to regulate promoter-proximal pause release and termination, respectively containing phosphatases PP4 a
Externí odkaz:
https://doaj.org/article/8e61f919126a433ba10ee6df1e7aa926
Autor:
Sampada Kalan, Ramon Amat, Miriam Merzel Schachter, Nicholas Kwiatkowski, Brian J. Abraham, Yanke Liang, Tinghu Zhang, Calla M. Olson, Stéphane Larochelle, Richard A. Young, Nathanael S. Gray, Robert P. Fisher
Publikováno v:
Cell Reports, Vol 21, Iss 2, Pp 467-481 (2017)
Summary: Cdk7, the CDK-activating kinase and transcription factor IIH component, is a target of inhibitors that kill cancer cells by exploiting tumor-specific transcriptional dependencies. However, whereas selective inhibition of analog-sensitive (AS
Externí odkaz:
https://doaj.org/article/6e79333bb0bb44d39aacfbac77a291d1
Supplemental Figure 1. A. FACS data showing DNA content of HeLaII transfected with the indicated siRNAs. B. Percentages of the cells found in subG1, G1, S, and G2/M for figure A (mean of four experiments). C. The p53BP1 foci do not show obvious co-lo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dcfc4930aa438dd5b3d5e9573a2ccafc
https://doi.org/10.1158/1541-7786.22517166
https://doi.org/10.1158/1541-7786.22517166
Supplemental Figure 4. A. IP-TRAP assays showing the precipitation of telomerase activity with antihTERT or anti-FEN1 antibodies, as well as the assays for OBFC1, p68 (POLA2) and POLα (p180). Both p68 and p180 are positive in this assay, where
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7d7d761a376e2a94d20c4c25ef3e6cd
https://doi.org/10.1158/1541-7786.22517154
https://doi.org/10.1158/1541-7786.22517154
Supplemental Figure 3. A. Extracts prepared from HTC75 cells expressing retrovirally transduced MYC-POLA2 were used for immunoprecipitations with the antibodies indicated at the top of the lanes. PI: preimmune serum, S: immune serum. The Western blot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54c3f8bfc428752c4a17be2ff3f9e670
https://doi.org/10.1158/1541-7786.22517157
https://doi.org/10.1158/1541-7786.22517157
Supplemental Figure 2. FACS profiles showing DNA content of the synchronized HeLaII at the indicated time points. These correspond to the experiment shown in 3C and 3D.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::063bf2419319ee7f8ade94fe6f8baf00
https://doi.org/10.1158/1541-7786.22517163
https://doi.org/10.1158/1541-7786.22517163
Supplemental Figure 6. A. Western blot for HeLaII transfected with the siRNAs indicated on top. The analysis was performed 48 hours post-transfection. B. Telomeric overhang analysis in HelaII depleted for 180 with the indicated siRNAs, using the DSN
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41ac53f4647b1cb6e7a8d29dc25523e3
https://doi.org/10.1158/1541-7786.22517148.v1
https://doi.org/10.1158/1541-7786.22517148.v1
Supplemental Figure 5. A. Western blot for HeLaII expressing stable p68 shRNA B. In-gel hybridization for HeLaII treated with shRNA for p68 after ten passages. B. Overhang intensity for shRNA samples, processed as described in Fig.5B. C. Quantitation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30ee8b9ba6475cec1638ffa837acc8a9
https://doi.org/10.1158/1541-7786.22517151
https://doi.org/10.1158/1541-7786.22517151
Figure legends to supplemental figures 1-6
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c6a0fd684c5fa8901e5aa48e168c9d4
https://doi.org/10.1158/1541-7786.22517145.v1
https://doi.org/10.1158/1541-7786.22517145.v1
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Nature Communications
Nature Communications
Reversible phosphorylation of Pol II and accessory factors helps order the transcription cycle. Here, we define two kinase-phosphatase switches that operate at different points in human transcription. Cdk9/cyclin T1 (P-TEFb) catalyzes inhibitory phos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0baeb2672a2e3b03516f6c0d790d449
https://doaj.org/article/8e61f919126a433ba10ee6df1e7aa926
https://doaj.org/article/8e61f919126a433ba10ee6df1e7aa926