Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Sam Philip Heywood"'
Autor:
Danielle Freches, Rita Vanbever, Catherine Uyttenhove, Maria Machado Franco, Sam Philip Heywood, Harshad P. Patil
Publikováno v:
International Journal of Pharmaceutics. 521:120-129
The PEGylation of antibody fragments has been shown to greatly prolong their residence time in the lungs in mice. The purpose of this research was to confirm the effect of PEGylation in higher animal species, that is, the rat and the rabbit. An anti-
Autor:
Alastair D. G. Lawson, Ralph Adams, Joanne E. Compson, Tim Kopotsha, Michael Airey, Hanna Hailu, David Paul Humphreys, Oliver Zaccheo, Kaushik Sarkar, Mark Jairaj, Wild Gavin Barry, Andrew M. Ventom, Sarah L. Dugdale, Louis Christodoulou, Emma Dave, Sarah Malcolm, Diane Marshall, Alison Turner, Bruce Carrington, James T. Heads, Miren Zamacona, Sam Philip Heywood
Publikováno v:
mAbs
An antibody format, termed Fab-dsFv, has been designed for clinical indications that require monovalent target binding in the absence of direct Fc receptor (FcR) binding while retaining substantial serum presence. The variable fragment (Fv) domain of
Autor:
Mark Jairaj, Emma Dave, Ralph Adams, Shauna West, Laura Griffin, Oliver Zaccheo, Sam Philip Heywood, David Paul Humphreys, Tom Ceska, Joanne E. Compson, Alastair Dg. Lawson, Terry Baker
Publikováno v:
mAbs
We generated an anti-albumin antibody, CA645, to link its Fv domain to an antigen-binding fragment (Fab), thereby extending the serum half-life of the Fab. CA645 was demonstrated to bind human, cynomolgus, and mouse serum albumin with similar affinit
Autor:
Rita Vanbever, Sam Philip Heywood, Salome Juliette Koussoroplis, Jacques Van Snick, Catherine Uyttenhove, Céline Barilly
Publikováno v:
International Journal of Pharmaceutics. 454:107-115
The aim of this study was to maximize the yield of the production of mono-PEGylated anti-interleukin-17A (anti-IL-17A) antibody fragments using large (≥ 20 kDa) polyethylene glycol (PEG) chains. Particular attention was paid to selectively yield mo
Autor:
Sarah E Lane, Leigh C Bowering, Sam Philip Heywood, David Paul Humphreys, Lloyd M. King, James P Turner
Publikováno v:
Protein Expression and Purification. 37:109-118
Escherichia coli is a widely used host for the heterologous expression of proteins of therapeutic and commercial interest. The scale and speed at which it can be cultured can result in the rapid generation of large quantities of product. However, to
Publikováno v:
PLoS ONE, Vol 12, Iss 8, p e0182695 (2017)
PLoS ONE
PLoS ONE
A major role for FcRn is the salvage of pinocytosed IgG and albumin from a degradative fate in lysosomes. FcRn achieves this by binding IgG in a pH-dependent manner in acidic endosomes and recycling it to the plasma membrane to be released at neutral
Autor:
Eve E. Weatherill, James T. Heads, Ralph Adams, Katharine Cain, Joanne E. Compson, David Paul Humphreys, Sam Philip Heywood
Publikováno v:
Protein engineering, designselection : PEDS. 25(7)
Engineered introduction of interface interchain disulphide bonds is perceived to be a simple method to increase the stability of single chain Fv (scFv). Six disulphide bond locations have been cited within the literature but the potential for the bro
Autor:
Roger Palframan, Kevin Greenslade, Helen A. Brand, David Paul Humphreys, Bruce Carrington, Alistair James Henry, Dominc G. Reeks, P. Antoniw, Shauna West, Leigh C Bowering, Layla Ait-Lhadj, Sam Philip Heywood
Publikováno v:
Protein engineering, designselection : PEDS. 20(5)
Antigen-binding fragments (Fab') of antibodies can be site specifically PEGylated at thiols using cysteine reactive PEG-maleimide conjugates. For therapeutic Fab'-PEG, conjugation with 40 kDa of PEG at a single hinge cysteine has been found to confer
Investigation of the Distribution and Elimination of the PEG Component of Certolizumab Pegol in Rats
Publikováno v:
American Journal of Gastroenterology. 103:S431-S432