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DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments

Autor: Charles R. Connell, Nicolette D. Halloran, Richard D. Cheng, Linda G. Lee, Carl W. Fuller, Rick K. Wilson, Sam Lee Woo, Bernard F. McArdle

Publikováno v: Nucleic acids research. 20(10)

The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f12e20e63358d39b38938e637ac04127
https://pubmed.ncbi.nlm.nih.gov/1598205

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