Zobrazeno 1 - 10
of 47
pro vyhledávání: '"Salvatore Lanzavecchia"'
Publikováno v:
PLoS ONE, Vol 6, Iss 8, p e23753 (2011)
Lens transparency depends on the accumulation of massive quantities (600-800 mg/ml) of twelve primary crystallines and two truncated crystallines in highly elongated "fiber" cells. Despite numerous studies, major unanswered questions are how this het
Externí odkaz:
https://doaj.org/article/586ccb2d10ee42369dc297f36e45653d
Publikováno v:
Journal of Structural Biology. 169:192-199
We present a strategy for the alignment of dual-axis tomographic series, based on reference points and simultaneous alignment of both series. Each series is first aligned individually, an affine transformation is determined to bring the two series in
Autor:
Joel L. Rosenbaum, Francesca Cantele, Pietro Lupetti, Eugenio Paccagnini, Gaia Pigino, Salvatore Lanzavecchia, Stefan Geimer, Dennis R. Diener
Publikováno v:
The Journal of Cell Biology
Ultrastructural study of Chlamydomonas cilia shows that anterograde IFT particles form trains that are long and narrow, while retrograde IFT form short, compact particle trains.
Intraflagellar transport (IFT) is the bidirectional movement of mul
Intraflagellar transport (IFT) is the bidirectional movement of mul
Autor:
Lorenzo Zampighi, Guido A. Zampighi, Eleonora Salvi, Francesca Cantele, N. Fain, Salvatore Lanzavecchia, Gaia Pigino
Publikováno v:
Journal of Structural Biology. 161:287-297
We are presenting a program for interactive segmentation of tomographic maps, based on objective criteria so as to yield reproducible results. The strategy starts with the automatic segmentation of the entire volume with the watershed algorithm in 3D
Autor:
Francesca Cantele, Stephen D. Fuller, Twan Rutten, Roger M. Burnett, Salvatore Lanzavecchia, Carmen San Martín, Roberto Marabini, José María Carazo, Sjors H.W. Scheres
Publikováno v:
Journal of Structural Biology. 151:79-91
We present a novel strategy for classification of heterogeneous electron microscopy data of icosahedral virus particles. The effectiveness of the procedure, which is based on classification of single-projection reconstructions (SPRs), is first invest
Autor:
Francesca Cantele, David Mercati, Caterina Mencarelli, Romano Dallai, Salvatore Lanzavecchia, Pietro Lupetti
Publikováno v:
Cell Motility and the Cytoskeleton. 62:69-83
We present here for the first time a 3D reconstruction of in situ axonemal outer dynein arms. This reconstruction has been obtained by electron tomography applied to a series of tilted images collected from metal replicas of rapidly frozen, cryofract
Publikováno v:
Journal of Structural Biology. 141:84-92
A model-free method to determine the three-dimensional structure of icosahedral viruses is described. The novel strategy is based upon the approximate principle that correct virus structures have high variance as do all other well-detailed structures
Autor:
F. G. van der Goot, Yulia Tsitrin, Marie-Claire Velluz, Patrick Paumard, Catherine El Bez, Michael W. Parker, Salvatore Lanzavecchia, Jacques Dubochet, Marc Adrian, Craig J. Morton
Publikováno v:
Nature Structural Biology. 9:729-733
Proteins exist in one of two generally incompatible states: either membrane associated or soluble. Pore-forming proteins are exceptional because they are synthesized as a water-soluble molecule but end up being located in the membrane -- that is, the
Publikováno v:
Journal of Structural Biology. 137:259-272
In this paper we discuss the embedding of symmetry information in an algorithm for three-dimensional reconstruction, which is based on the discrete Radon transform. The original algorithm was designed for randomly oriented and in principal asymmetric
Publikováno v:
Ultramicroscopy. 87:187-197
The lines of a large group of sinograms of projections with random orientation can be submitted to correspondence analysis. Since the number of samples per line is small, a small matrix is accumulated which is quick to orthogonalise. In the eigenvect