Zobrazeno 1 - 10
of 88
pro vyhledávání: '"Salpingoeca rosetta"'
Autor:
Justine M Pinskey, Adhya Lagisetty, Long Gui, Nhan Phan, Evan Reetz, Amirrasoul Tavakoli, Gang Fu, Daniela Nicastro
Publikováno v:
eLife, Vol 11 (2022)
In most eukaryotic organisms, cilia and flagella perform a variety of life-sustaining roles related to environmental sensing and motility. Cryo-electron microscopy has provided considerable insight into the morphology and function of flagellar struct
Externí odkaz:
https://doaj.org/article/ef50ee5d98b74cc2a6b8474d82bf8e5c
Autor:
Thibaut Brunet, Marvin Albert, William Roman, Maxwell C Coyle, Danielle C Spitzer, Nicole King
Publikováno v:
eLife, Vol 10 (2021)
Amoeboid cell types are fundamental to animal biology and broadly distributed across animal diversity, but their evolutionary origin is unclear. The closest living relatives of animals, the choanoflagellates, display a polarized cell architecture (wi
Externí odkaz:
https://doaj.org/article/eb91b66f99144b5abd0c4b481927da0d
Synthesis of the CCA end of essential tRNAs is performed either by CCA-adding enzymes or as a collaboration between enzymes restricted to CC- and A-incorporation. While the occurrence of such tRNA nucleotidyltransferases with partial activities seeme
Externí odkaz:
https://ul.qucosa.de/id/qucosa%3A89302
https://ul.qucosa.de/api/qucosa%3A89302/attachment/ATT-0/
https://ul.qucosa.de/api/qucosa%3A89302/attachment/ATT-0/
Autor:
David S Booth, Nicole King
Publikováno v:
eLife, Vol 9 (2020)
In a previous study, we established a forward genetic screen to identify genes required for multicellular development in the choanoflagellate, Salpingoeca rosetta (Levin et al., 2014). Yet, the paucity of reverse genetic tools for choanoflagellates h
Externí odkaz:
https://doaj.org/article/c6a394c9e7de454997c6c61f83320a16
Publikováno v:
eLife
Amoeboid cell types are fundamental to animal biology and broadly distributed across animal diversity, but their evolutionary origin is unclear. The closest living relatives of animals, the choanoflagellates, display a polarized cell architecture (wi
Autor:
Nicole King, David S. Booth
Choanoflagellates, the closest living relatives of animals, have the potential to reveal the genetic and cell biological foundations of complex multicellular development in animals. Here we describe the history of research on the choanoflagellate Sal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::124ae2a9c7d73732f5d63f0c9f39b30c
https://doi.org/10.20944/preprints202109.0264.v1
https://doi.org/10.20944/preprints202109.0264.v1
Autor:
David Booth
This protocol details the preparation and execution of CRISPR/Cas9 genome editing in S. rosetta. The protocol builds on a method to transfect macromolecules into S. rosetta for delivering a purified Cas9 ribonucleoprotein from Streptomyces pyogenes (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6b720b2cd25ea9a9dad7282c3d720133
https://doi.org/10.17504/protocols.io.bx58pq9w
https://doi.org/10.17504/protocols.io.bx58pq9w
Autor:
Alex de Mendoza, Iñaki Ruiz-Trillo
Publikováno v:
eLife, Vol 3 (2014)
A genetic screen has revealed one of the molecules that allow choanoflagellates, the closest unicellular relative of animals, to form colonies, which could help researchers to answer questions about the earliest days of animal evolution.
Externí odkaz:
https://doaj.org/article/6431e801df66405d89dd047bc10e192a
Publikováno v:
eLife, Vol 3 (2014)
The origin of animal multicellularity may be reconstructed by comparing animals with one of their closest living relatives, the choanoflagellate Salpingoeca rosetta. Just as animals develop from a single cell–the zygote–multicellular rosettes of
Externí odkaz:
https://doaj.org/article/5ba6b8b6edff460993c028ae99de9956
Publikováno v:
Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
Horizontal gene transfer (HGT), the movement of heritable materials between distantly related organisms, is crucial in eukaryotic evolution. However, the scale of HGT in choanoflagellates, the closest unicellular relatives of metazoans, and its possi