Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Sakurako Ichinose"'
Autor:
Toshiharu Arai, Sakurako Ichinose, Nozomu Shibata, Hiroshi Kakeshita, Hiroshi Kodama, Kazuaki Igarashi, Yasushi Takimura
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-14 (2022)
Abstract Trichoderma reesei is a widely used host for producing cellulase and hemicellulase cocktails for lignocellulosic biomass degradation. Here, we report a genetic modification strategy for industrial T. reesei that enables enzyme production usi
Externí odkaz:
https://doaj.org/article/7bd3dd5ee9014dc589673661cf4a4555
Autor:
Toshiharu Arai, Sakurako Ichinose, Nozomu Shibata, Hiroshi Kakeshita, Hiroshi Kodama, Kazuaki Igarashi, Yasushi Takimura
Background: Trichoderma reesei (Hypocrea jecorina) is a filamentous fungus that can produce extremely high levels of protein; consequently, it is utilized as a host for the production of cellulase and hemicellulase cocktails for lignocellulosic bioma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6a8a476204a939bcbcca4f25073872e
https://doi.org/10.21203/rs.3.rs-1200930/v1
https://doi.org/10.21203/rs.3.rs-1200930/v1
Publikováno v:
Molecular microbiology. 110(2)
Carbon catabolite repression (CCR) is regulated by the C2 H2 -type transcription factor CreA/Cre1 in filamentous fungi including Aspergillus oryzae. We investigated the stability and subcellular localization of CreA in A. oryzae. The abundance of FLA
Autor:
Katsuya Gomi, Kuta Suzuki, Takanori Ichikawa, Mizuki Tanaka, Sakurako Ichinose, Takahiro Shintani, Yui Konno, Sachiko Hasegawa-Shiro
Publikováno v:
Applied Microbiology and Biotechnology. 99:1805-1815
The production of amylolytic enzymes in Aspergillus oryzae is induced in the presence of starch or maltose, and two Zn2Cys6-type transcription factors, AmyR and MalR, are involved in this regulation. AmyR directly regulates the expression of amylase
Publikováno v:
Journal of bioscience and bioengineering. 125(2)
In a previous study, we reported that a double gene deletion mutant for CreA and CreB, which constitute the regulatory machinery involved in carbon catabolite repression, exhibited improved production of α-amylase compared with the wild-type strain
Autor:
Hiroki Sato, Sakurako Ichinose, Katsuya Gomi, Mizuki Tanaka, Silai Zhang, Takahiro Shintani, Ken Miyazawa, Keietsu Abe, Akira Yoshimi
Publikováno v:
Journal of bioscience and bioengineering. 124(1)
We have previously reported that α-amylase (Taka-amylase A, TAA) activity disappears in the later stage of submerged Aspergillus oryzae culture as a result of TAA adsorption onto the cell wall. Chitin, one of the major components of the cell wall, w
Publikováno v:
Applied Microbiology and Biotechnology. 98:335-343
In filamentous fungi, the expression of secretory glycoside hydrolase encoding genes, such as those for amylases, cellulases, and xylanases, is generally repressed in the presence of glucose. CreA and CreB have been observed to be regulating factors
Autor:
Sakurako Ichinose, Dararat Kakizono, Katsuya Gomi, Daisuke Shiro, Osamu Mizutani, Takahiro Shintani, Yasumichi Onozaki, Tomoko Shintani, Jun ichi Yokota, Mizuki Tanaka
Publikováno v:
Applied microbiology and biotechnology. 101(6)
Filamentous fungi are often used as cell factories for recombinant protein production because of their ability to secrete large quantities of hydrolytic enzymes. However, even using strong transcriptional promoters, yields of nonfungal proteins are g