Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Sagar Khavnekar"'
Autor:
Noushin Hajarolasvadi, Vikram Sunkara, Sagar Khavnekar, Florian Beck, Robert Brandt, Daniel Baum
Publikováno v:
BMC Bioinformatics, Vol 23, Iss 1, Pp 1-16 (2022)
Abstract Background Despite recent advances in cellular cryo-electron tomography (CET), developing automated tools for macromolecule identification in submolecular resolution remains challenging due to the lack of annotated data and high structural c
Externí odkaz:
https://doaj.org/article/0b83bdb32ec846448dd7d390138cc366
Autor:
Philipp S. Erdmann, Zhen Hou, Sven Klumpe, Sagar Khavnekar, Florian Beck, Florian Wilfling, Jürgen M. Plitzko, Wolfgang Baumeister
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-9 (2021)
The large and small subunits of the ribosome are synthesized independently within the nucleolus — a membrane-less compartment within the nucleus — before being exported into the cytoplasm. Here, the authors use in situ cryo-ET to observe ribosome
Externí odkaz:
https://doaj.org/article/29af8d2ce12c4b48a297291bce32bf53
Autor:
Sagar Khavnekar, Veronika Vrbovská, Magda Zaoralová, Ron Kelley, Florian Beck, Sven Klumpe, Abhay Kotecha, Jürgen Plitzko, Philipp S. Erdmann
We here present a method based on metallic platinum sputtering that can substantially enhance the quality of subtomogram averages from lamellas and thereby reduce the number of particles needed for high-resolution subtomogram averaging. We provide ev
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9b16c3caa7902447ba62e6395a175bd9
https://doi.org/10.1101/2022.06.16.496417
https://doi.org/10.1101/2022.06.16.496417
Autor:
Sagar Khavnekar, Wojciech Wietrzynski, William Wan, Juergen Plitzko, Philipp Erdmann, Parijat Majumder
Cryo-electron tomography (cryo-ET) and subtomogram averaging (STA) can resolve protein complexes at near atomic resolution, and when combined with focused ion beam (FIB) milling, macromolecules can be observed within their native context. Unlike sing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f538d5efa674751c3989c336b696165e
https://doi.org/10.1101/2022.04.10.487763
https://doi.org/10.1101/2022.04.10.487763
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
Autor:
Daniel Němeček, Abhay Kotecha, Oliver Raschdorf, Fanis Grollios, Rob van der Ploeg, Dennis Cats, Dimple Karia, Wen Yang, Sagar Khavnekar, Adrian Koh
Publikováno v:
Journal of visualized experiments : JoVE. (181)
Cryo-electron microscopy (cryo-EM) has been established as a routine method for protein structure determination during the past decade, taking an ever-increasing share of published structural data. Recent advances in TEM technology and automation hav
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3002f040cae591a1ff3fd41b3c74609a
https://pubmed.ncbi.nlm.nih.gov/35377368
https://pubmed.ncbi.nlm.nih.gov/35377368
Autor:
Sagar Khavnekar, Jürgen M. Plitzko, Zhen Hou, Florian Beck, Sven Klumpe, Florian Wilfling, Philipp Erdmann, Wolfgang Baumeister
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-9 (2021)
Ribosomes comprise a large (LSU) and a small subunit (SSU) which are synthesized independently in the nucleolus before being exported into the cytoplasm, where they assemble into functional ribosomes. Individual maturation steps have been analyzed in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::531c391bb69dfa554387876c28f6e3c8
https://doi.org/10.1038/s41467-021-25413-w
https://doi.org/10.1038/s41467-021-25413-w
Publikováno v:
Microscopy and Microanalysis. 27:3240-3240
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::455b33c5d8f541a347c2f9027a9a4607
https://doi.org/10.1017/s143192762101117x
https://doi.org/10.1017/s143192762101117x
Autor:
Sagar Khavnekar, John B. Rafferty, Sarath Chandra Dantu, Svetlana E. Sedelnikova, Sylvia Ayora, Avinash Kale
Publikováno v:
Nucleic Acids Research
'Nucleic Acids Research ', vol: 45, pages: 975-986 (2017)
Europe PubMed Central
'Nucleic Acids Research ', vol: 45, pages: 975-986 (2017)
Europe PubMed Central
© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. Holliday junction (HJ) resolving enzyme RecU is involved in DNA repair and recombination. We have determined the crystal structure of inactive mutant (D8
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b0b8d04981384dc09466b2ad40f9f3e
http://europepmc.org/articles/PMC5314769
http://europepmc.org/articles/PMC5314769
Publikováno v:
Journal of Biomolecular Structure and Dynamics. 35:2186-2196
Peb4 from Campylobacter jejuni is an intertwined dimeric, periplasmic holdase, which also exhibits peptidyl prolyl cis/trans isomerase (PPIase) activity. Peb4 gene deletion alters the outer membrane protein profile and impairs cellular adhesion and b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::286b24e1e39c52e1eb983c86be79ef0e
https://doi.org/10.1080/07391102.2016.1209131
https://doi.org/10.1080/07391102.2016.1209131
Publikováno v:
Microscopy and Microanalysis. 26:2516-2516
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0545a97094a2fe79b185c25f70e7de70
https://doi.org/10.1017/s143192762002187x
https://doi.org/10.1017/s143192762002187x