Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Saccharomycetales/genetics"'
Publikováno v:
STAR protocols, 3(2):101358
CRISPR/Cas9 technology allows accurate, marker-less genome editing. We report a detailed, robust, and streamlined protocol for CRISPR/Cas9 genome editing in Saccharomyces cerevisiae, based on the widely used MoClo-Yeast Toolkit (https://www.addgene.o
Publikováno v:
Mircen Journal of Applied Microbiology and Biotechnology, 38(3). Springer Science+Business Media
The conventional baker's yeast, Saccharomyces cerevisiae, is the indispensable baking yeast of all times. Its monopoly coupled to its major drawbacks, such as streamlined carbon substrate utilisation base and a poor ability to withstand a number of b
Autor:
Sophie Bamps, Arno Pihlak, Thomas Westerling, Lionel Tafforeau, Damien Hermand, Tomi P. Mäkelä, Jean Vandenhaute
Publikováno v:
Biochemical and biophysical research communications, 325 (4
The Mcs6 CDK together with its cognate cyclin Mcs2 represents the CDK-activating kinase (CAK) of fission yeast Cdc2. We have attempted to determine complexes in which Mcs6 and Mcs2 mediate this and possible other functions. Here we characterize a nov
Autor:
Gilles Fischer, Martial Marbouty, Gianni Liti, Christophe Zimmer, Hervé Marie-Nelly, Axel Cournac, Romain Koszul
Publikováno v:
Bioinformatics
Bioinformatics, 2014, 30 (15), pp.2105-13. ⟨10.1093/bioinformatics/btu162⟩
Bioinformatics, Oxford University Press (OUP), 2014, 30 (15), pp.2105-13. ⟨10.1093/bioinformatics/btu162⟩
Bioinformatics, 2014, 30 (15), pp.2105-13. ⟨10.1093/bioinformatics/btu162⟩
Bioinformatics, Oxford University Press (OUP), 2014, 30 (15), pp.2105-13. ⟨10.1093/bioinformatics/btu162⟩
Motivations: De novo sequencing of genomes is followed by annotation analyses aiming at identifying functional genomic features such as genes, non-coding RNAs or regulatory sequences, taking advantage of diverse datasets. These steps sometimes fail a
Publikováno v:
PloS one, 6 (9
PLoS ONE, Vol 6, Iss 9, p e24962 (2011)
PLoS ONE
PLoS ONE, Vol 6, Iss 9, p e24962 (2011)
PLoS ONE
Ribogenesis is a multistep error-prone process that is actively monitored by quality control mechanisms. How ribosomal RNA synthesis, pre-rRNA processing and nucleolar surveillance are integrated is unclear. Nor is it understood how defective ribosom
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13f52ddc2f9b014595aaf0717c5d7b04
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/110851
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/110851
Publikováno v:
Molecular Genetics and Genomics
Europe PubMed Central
Molecular Genetics and Genomics, vol. 291, no. 6, pp. 2231-2240
Europe PubMed Central
Molecular Genetics and Genomics, vol. 291, no. 6, pp. 2231-2240
The tractability of the budding yeast genome has provided many insights into the fundamental mechanisms regulating cellular life. With the advent of synthetic biol ogy and single cell measurements novel tools are required to manipulate the yeast geno