Zobrazeno 1 - 10
of 140
pro vyhledávání: '"SaCas9"'
Autor:
Zhi-Xue Yang, Ya-Wen Fu, Juan-Juan Zhao, Feng Zhang, Si-Ang Li, Mei Zhao, Wei Wen, Lei Zhang, Tao Cheng, Jian-Ping Zhang, Xiao-Bing Zhang
Publikováno v:
Genomics, Proteomics & Bioinformatics, Vol 21, Iss 6, Pp 1206-1220 (2023)
A series of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9) systems have been engineered for genome editing. The most widely used Cas9 is SpCas9 from Streptococcus pyogenes and SaCas9 from Staphyl
Externí odkaz:
https://doaj.org/article/dd9a845d89064b8db6bec0665d9eb342
Autor:
Khaled Essawi, Waleed Hakami, Muhammad Behroz Naeem Khan, Reid Martin, Jing Zeng, Rebecca Chu, Naoya Uchida, Aylin C. Bonifacino, Allen E. Krouse, Nathaniel S. Linde, Robert E. Donahue, Gerd A. Blobel, Ulrike Gerdemann, Leslie S. Kean, Stacy A. Maitland, Scot A. Wolfe, Jean-Yves Metais, Stephen Gottschalk, Daniel E. Bauer, John F. Tisdale, Selami Demirci
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 29, Iss , Pp 483-493 (2023)
CRISPR-Cas9-based therapeutic genome editing approaches hold promise to cure a variety of human diseases. Recent findings demonstrate pre-existing immunity for the commonly used Cas orthologs from Streptococcus pyogenes (SpCas9) and Staphylococcus au
Externí odkaz:
https://doaj.org/article/186bb2a612c54f99a14f07e9473590ec
Publikováno v:
Cell & Bioscience, Vol 12, Iss 1, Pp 1-10 (2022)
Abstract Background The true diploid frog, Xenopus tropicalis (X. tropicalis) is an excellent genetic model organism. To date, the CRISPR/Cas-mediated genome editing methods established in this species are mostly based on SpCas9 that requires the str
Externí odkaz:
https://doaj.org/article/58ea460350694baa96a04be859892a2b
Autor:
Yu Zhang, Takahiko Nishiyama, Hui Li, Jian Huang, Ayhan Atmanli, Efrain Sanchez-Ortiz, Zhaoning Wang, Alex A. Mireault, Pradeep P.A. Mammen, Rhonda Bassel-Duby, Eric N. Olson
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 22, Iss , Pp 122-132 (2021)
Duchenne muscular dystrophy (DMD), caused by mutations in the X-linked dystrophin gene, is a lethal neuromuscular disease. Correction of DMD mutations in animal models has been achieved by CRISPR/Cas9 genome editing using Streptococcus pyogenes Cas9
Externí odkaz:
https://doaj.org/article/f687232ad75d4bad97c0326b10f1b620
Autor:
Yuxi Chen, Shengyao Zhi, Puping Liang, Qi Zheng, Mengni Liu, Qi Zhao, Jian Ren, Jun Cui, Junjiu Huang, Yizhi Liu, Zhou Songyang
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 18, Iss , Pp 33-43 (2020)
Herpes simplex keratitis (HSK) is the most common cause of corneal blindness in developed nations, caused by primary or recurrent herpes simplex virus 1 (HSV-1) infection of the cornea. Latent infection of HSV-1, especially in the trigeminal ganglion
Externí odkaz:
https://doaj.org/article/ddf891fa45ea41bf9006415f60ec22bb
Autor:
Shuai Wang, Huilin Mao, Linghui Hou, Ziying Hu, Yao Wang, Tao Qi, Chen Tao, Yuan Yang, Chengdong Zhang, Miaomiao Li, Huihui Liu, Shijun Hu, Renjie Chai, Yongming Wang
Publikováno v:
Advanced Science, Vol 9, Iss 4, Pp n/a-n/a (2022)
Abstract Clustered regularly interspaced short palindromic repeat (CRISPR)/SaCas9 is the most popular tool for in vivo genome editing due to its high efficiency and small genome. The authors previously developed four SaCas9 orthologs as genome‐edit
Externí odkaz:
https://doaj.org/article/65cf145de86a4625822dec403823eaab
Publikováno v:
Frontiers in Genome Editing, Vol 2 (2021)
Biotic diseases cause substantial agricultural losses annually, spurring research into plant pathogens and strategies to mitigate them. Nicotiana benthamiana is a commonly used model plant for studying plant–pathogen interactions because it is host
Externí odkaz:
https://doaj.org/article/b5d09718a2164e29884d281645d209a3
Autor:
Yan Feng, Cheng Chen, Yuxiang Han, Zelin Chen, Xiaochan Lu, Fang Liang, Song Li, Wei Qin, Shuo Lin
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 6, Iss 8, Pp 2517-2521 (2016)
The type II CRISPR/Cas9 system has been used widely for genome editing in zebrafish. However, the requirement for the 5′-NGG-3′ protospacer-adjacent motif (PAM) of Cas9 from Streptococcus pyogenes (SpCas9) limits its targeting sequences. Here, we
Externí odkaz:
https://doaj.org/article/9a927181f14a449bbe09c6db6d48fb3a
Autor:
A. Katrin Helfer-Hungerbuehler, Jimit Shah, Theres Meili, Eva Boenzli, Pengfei Li, Regina Hofmann-Lehmann
Publikováno v:
Viruses, Vol 13, Iss 8, p 1636 (2021)
Feline leukemia virus (FeLV) is a retrovirus of cats worldwide. High viral loads are associated with progressive infection and the death of the host, due to FeLV-associated disease. In contrast, low viral loads, an effective immune response, and a be
Externí odkaz:
https://doaj.org/article/af6960a1499e465984bd054a3da4085f
Autor:
Salma G. Morsy, Jason M. Tonne, Yaxi Zhu, Brian Lu, Karol Budzik, James W. Krempski, Sherine A. Ali, Mohamed A. El-Feky, Yasuhiro Ikeda
Publikováno v:
BMC Research Notes, Vol 10, Iss 1, Pp 1-7 (2017)
Abstract Objective Recombinant adeno-associated virus (AAV)-based vectors are characterized by their robust and safe transgene delivery. The CRISPR/Cas9 and guide RNA (gRNA) system present a promising genome-editing platform, and a recent development
Externí odkaz:
https://doaj.org/article/08fb99c2192749669f3d9f97c87e48b9