Efficient genome editing in medaka ( Oryzias latipes ) using a codon-optimized SaCas9 system.

Autor: Jiang Y; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China., Pan Q; State Key Laboratory of Mariculture Breeding, Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College of Jimei University, Xiamen 361021, China., Wang Z; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China., Lu K; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China., Xia B; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China., Chen T; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. tiansheng.chen@jmu.edu.cn.; State Key Laboratory of Mariculture Breeding, Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College of Jimei University, Xiamen 361021, China. tiansheng.chen@jmu.edu.cn.

Publikováno v: Journal of Zhejiang University. Science. B [J Zhejiang Univ Sci B] 2024 Dec 15; Vol. 25 (12), pp. 1083-1096.

Superior Fidelity and Distinct Editing Outcomes of SaCas9 Compared with SpCas9 in Genome Editing

Autor: Zhi-Xue Yang, Ya-Wen Fu, Juan-Juan Zhao, Feng Zhang, Si-Ang Li, Mei Zhao, Wei Wen, Lei Zhang, Tao Cheng, Jian-Ping Zhang, Xiao-Bing Zhang

Publikováno v: Genomics, Proteomics & Bioinformatics, Vol 21, Iss 6, Pp 1206-1220 (2023)

A series of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9) systems have been engineered for genome editing. The most widely used Cas9 is SpCas9 from Streptococcus pyogenes and SaCas9 from Staphyl

Externí odkaz: https://doaj.org/article/dd9a845d89064b8db6bec0665d9eb342

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Efficient genome editing in medaka (Oryzias latipes) using a codon-optimized SaCas9 system

Publikováno v: Journal of Zhejiang University - Science B; December 2024, Vol. 25 Issue: 12 p1083-1096, 14p

Allele-specific genome editing of imprinting genes by preferentially targeting non-methylated loci using Staphylococcus aureus Cas9 (SaCas9)

Autor: Liu, Yajing, Li, Jianan, Zhou, Changyang, Meng, Bin, Wei, Yu, Yang, Guang, Lu, Zongyang, Shen, Qingmei, Zhang, Yu, Yang, Hui, Qiao, Yunbo

Publikováno v: In Science Bulletin 15 November 2019 64(21):1592-1600