[Biological microchip for identification of orthopoxviruses and agents causing the same clinical picture as in smallpox]

Autor: Ai, Myznikova, Sa, Lapa, Dmitry Gryadunov, Da, Khodakov, As, Zasedatelev, Vm, Mikhaĭlovich

Publikováno v: Europe PubMed Central

A method based on hybridization of simultaneously amplified gene fragments of orthopoxviruses and herpesviruses with oligonucleotide probes immobilized on a microarray has been developed. The method permits identification of 6 orthopoxvirus species a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::f6267a250aa9706870851ab67637843d
https://pubmed.ncbi.nlm.nih.gov/17500239

[Identification of Mycobacterium tuberculosis strains and a simultaneous identification of their drug resistance by the hybridization method on oligonucleotide microchips]

Autor: Dmitry Gryadunov, Vm, Mikhaĭlovich, Sa, Lapa, Ni, Rudinskiĭ, Ve, Barskiĭ, Av, Chudinov, As, Zasedatelev, Ad, Mirzabekov

Publikováno v: Europe PubMed Central

A method of multiplex polymerase chain reaction (PCR) with subsequent hyoridization on oligonucleotide microchips was worked out to identify the Mycobacterium tuberculosis complex and to determine simultaneously the bacterial sensitivity to 2 first-l

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::ad5ef30a2a34588a753da89c90ee109c
http://europepmc.org/abstract/med/14664159

Combining recombinase polymerase amplification with tyrosine modified 2'-deoxyuridine-5'-triphosphate for direct voltammetric detection of double-stranded DNA: Application to potato pathogen Dickeya solani.

Autor: Suprun EV; Chemistry Faculty of M.V. Lomonosov Moscow State University, Lenin Hills, 1/3, Moscow, 119991, Russia; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow, 119121, Russia. Electronic address: lenasuprun@mail.ru., Khmeleva SA; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow, 119121, Russia., Duskaev IF; Chemistry Faculty of M.V. Lomonosov Moscow State University, Lenin Hills, 1/3, Moscow, 119991, Russia; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow, 119121, Russia., Ptitsyn KG; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow, 119121, Russia., Kurbatov LK; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow, 119121, Russia., Shershov VE; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street, 32, Moscow, 119991, Russia., Kuznetsova VE; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street, 32, Moscow, 119991, Russia., Lapa SA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street, 32, Moscow, 119991, Russia., Chudinov AV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street, 32, Moscow, 119991, Russia., Radko SP; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow, 119121, Russia.

Publikováno v: Talanta [Talanta] 2024 Jun 01; Vol. 273, pp. 125841. Date of Electronic Publication: 2024 Feb 29.

Synthesis of Cy5-Labelled C5-Alkynyl-modified cytidine triphosphates via Sonogashira coupling for DNA labelling.

Autor: Kuznetsova VE; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia. Electronic address: kuzneimb@gmail.com., Shershov VE; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Guseinov TO; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Miftakhov RA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Solyev PN; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Novikov RA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Levashova AI; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Zasedatelev AS; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Lapa SA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Chudinov AV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia. Electronic address: chud@eimb.ru.

Publikováno v: Bioorganic chemistry [Bioorg Chem] 2023 Feb; Vol. 131, pp. 106315. Date of Electronic Publication: 2022 Dec 10.

[Study of Multiple Enzymatic Incorporation of Modified Nucleotides of Purine and Pyrimidine Nature in the Growing DNA Chain].

Autor: Lapa SA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.; lapa@biochip.ru., Volkova OS; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Kuznetsova VE; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Zasedatelev AS; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Chudinov AV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.

Publikováno v: Molekuliarnaia biologiia [Mol Biol (Mosk)] 2022 Jan-Feb; Vol. 56 (1), pp. 157-167.