Crystal structure of the catalytic domain of botulinum neurotoxin subtype A3

Autor: Yufan Wu, Oneda Leka, Richard A. Kammerer, Xiao-Dan Li

Publikováno v: The Journal of Biological Chemistry

Botulinum neurotoxins (BoNTs) are among the most widely used therapeutic proteins; however, only two subtypes within the seven serotypes, BoNT/A1 and BoNT/B1, are currently used for medical and cosmetic applications. Distinct catalytic properties, su

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f63f4a9422b97519a0db73c3472043f3
http://europepmc.org/articles/PMC8135040

Inhibiting glycolysis rescues memory impairment in an intellectual disability Gdi1-null mouse

Autor: Maja Malnar, Angela Bachi, Helena H. Chowdhury, Anemari Horvat, Patrizia D’Adamo, Antonia Gurgone, Saša Trkov Bobnar, Marko Muhič, Lorenzo Piemonti, Maria Lidia Mignogna, Michela Masetti, Jelena Velebit, Veronica Bianchi, Robert Zorec, Matjaž Stenovec, Alessia Mercalli, Katja Fink, Sara Belloli, Stefano Taverna, Maja Potokar, Marko Kreft, Rosa Maria Moresco, Nina Vardjan, Maddalena Ripamonti, Umberto Restuccia

Publikováno v: Metabolism
Metabolism, clinical and experimental
116 (2021): 154463. doi:10.1016/j.metabol.2020.154463
info:cnr-pdr/source/autori:D'Adamo P.; Horvat A.; Gurgone A.; Mignogna M.L.; Bianchi V.; Masetti M.; Ripamonti M.; Taverna S.; Velebit J.; Malnar M.; Muhic M.; Fink K.; Bachi A.; Restuccia U.; Belloli S.; Moresco R.M.; Mercalli A.; Piemonti L.; Potokar M.; Bobnar S.T.; Kreft M.; Chowdhury H.H.; Stenovec M.; Vardjan N.; Zorec R./titolo:Inhibiting glycolysis rescues memory impairment in an intellectual disability Gdi1-null mouse/doi:10.1016%2Fj.metabol.2020.154463/rivista:Metabolism, clinical and experimental (Print)/anno:2021/pagina_da:154463/pagina_a:/intervallo_pagine:154463/volume:116

Objectives GDI1 gene encodes for αGDI, a protein controlling the cycling of small GTPases, reputed to orchestrate vesicle trafficking. Mutations in human GDI1 are responsible for intellectual disability (ID). In mice with ablated Gdi1, a model of ID

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2b634b090f73e01f5e64f5a419978ac
http://europepmc.org/articles/PMC7871014

Preparation of dissociated mouse primary neuronal cultures from long-term cryopreserved brain tissue

Autor: Umesh Vivekananda, M. Cano-Jaimez, P.R.F. Mendonca, Erica Tagliatti, Kirill E. Volynski, Dimitri M. Kullmann, E. Nicholson

Publikováno v: Journal of Neuroscience Methods

Highlights • Primary neuronal cultures from cryopreserved tissue retain functional neuronal properties. • Cryopreservation of brain tissue allows long-distance collaborations. • Exchange of cryopreserved tissue can reduce the need to maintain m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c392d58d460c681b1c6b32640440e32
http://europepmc.org/articles/PMC7026713

Simultaneous monitoring of three key neuronal functions in primary neuronal cultures

Autor: Gareth J.O. Evans, Michael A. Cousin

Publikováno v: Evans, G J O & Cousin, M A 2007, ' Simultaneous monitoring of three key neuronal functions in primary neuronal cultures ', Journal of Neuroscience Methods, vol. 160, no. 2, pp. 197-205 . https://doi.org/10.1016/j.jneumeth.2006.09.012
Journal of Neuroscience Methods

The coupling of Ca(2+) influx to synaptic vesicle (SV) recycling in nerve terminals is essential for neurotransmitter release and thus neuronal communication. Both of these parameters have been monitored using fluorescent reporter dyes such as fura-2

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ca95be992de3b0418b9d3bb04a65c6c
https://doi.org/10.1016/j.jneumeth.2006.09.012