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Comparison of EV characterization by commercial high-sensitivity flow cytometers and a custom single-molecule flow cytometer.

Autor: Kim J; Department of Chemistry, University of Washington, Seattle, Washington, USA., Xu S; Department of Chemistry, University of Washington, Seattle, Washington, USA., Jung SR; Department of Chemistry, University of Washington, Seattle, Washington, USA., Nguyen A; Department of Chemistry, University of Washington, Seattle, Washington, USA., Cheng Y; Department of Chemistry, University of Washington, Seattle, Washington, USA., Zhao M; Department of Chemistry, University of Washington, Seattle, Washington, USA., Fujimoto BS; Department of Chemistry, University of Washington, Seattle, Washington, USA., Nelson W; Department of Chemistry, University of Washington, Seattle, Washington, USA., Schiro P; Micareo Inc, Burlingame, California, USA., Franklin JL; Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA., Higginbotham JN; Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA., Coffey RJ; Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA.; Department of Cell Biology, Vanderbilt University, Nashville, Tennessee, USA., Shi M; Department of Pathology, University of Washington, Seattle, Washington, USA., Vojtech LN; Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA., Hladik F; Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA.; Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA., Tewari M; Division of Hematology/Oncology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA.; Rogel Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michigan, USA.; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.; Center for Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, Michigan, USA.; VA Ann Arbor Healthcare System, Ann Arbor, Michigan, USA., Tigges J; Department of Medicine, Beth Israel Deaconess Medical Center, Boston and Cambridge, Massachusetts, USA., Ghiran I; Department of Medicine, Beth Israel Deaconess Medical Center, Boston and Cambridge, Massachusetts, USA., Jovanovic-Talisman T; Department of Molecular Medicine, Beckman Research Institute of the City of Hope Comprehensive Cancer Center, Duarte, California, USA., Laurent LC; Department of Obstetrics, Gynecology, and Reproductive Sciences and Sanford Consortium for Regenerative Medicine, University of California San Diego, San Diego, California, USA., Das S; Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical school, Boston, Massachusetts, USA., Gololobova O; Department of Molecular and Comparative Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Witwer KW; Department of Molecular and Comparative Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Xu T; Cancer Research Institute, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.; Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA., Charest A; Cancer Research Institute, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.; Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA., Jensen KVK; Neurogenomics Division, TGen, Phoenix, Arizona, USA., Raffai RL; Department of Veterans Affairs, Surgical Service (112G), San Francisco VA Medical Center, San Francisco, California, USA., Jones JC; Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA., Welsh JA; Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA., Nolan JP; Scintillon Institute, San Diego, California, USA., Chiu DT; Department of Chemistry, University of Washington, Seattle, Washington, USA.

Publikováno v: Journal of extracellular vesicles [J Extracell Vesicles] 2024 Aug; Vol. 13 (8), pp. e12498.

Improving Multicolor Colocalization in Single-Vesicle Flow Cytometry with Vesicle Transit Time.

Autor: Andronico LA; Department of Women's and Children's Health (KBH), Karolinska Institutet, Solna 17177, Sweden., Jung SR; Department of Chemistry, University of Washington, Seattle, Washington 98195, United States., Fujimoto BS; Department of Chemistry, University of Washington, Seattle, Washington 98195, United States., Chiu DT; Department of Chemistry, University of Washington, Seattle, Washington 98195, United States.

Publikováno v: Analytical chemistry [Anal Chem] 2023 Jul 18; Vol. 95 (28), pp. 10492-10497. Date of Electronic Publication: 2023 Jul 05.

BODIPY-based near-infrared semiconducting polymer dot for selective yellow laser-excited cell imaging.

Autor: Chen L; Department of Biomedical Engineering, Sun Yat-Sen University Shenzhen 518107 China.; Department of Chemistry and Bioengineering, University of Washington Seattle Washington 98195 USA chiu@uw.edu., Jiang Y; Department of Chemistry and Bioengineering, University of Washington Seattle Washington 98195 USA chiu@uw.edu., Xu S; Department of Chemistry and Bioengineering, University of Washington Seattle Washington 98195 USA chiu@uw.edu., Zhang J; Department of Chemistry and Bioengineering, University of Washington Seattle Washington 98195 USA chiu@uw.edu., Jung SR; Department of Chemistry and Bioengineering, University of Washington Seattle Washington 98195 USA chiu@uw.edu., Yu J; Department of Chemistry and Bioengineering, University of Washington Seattle Washington 98195 USA chiu@uw.edu., Zhang X; Department of Health Sciences, University of Macau Taipa Macau SAR 999078 China., Chiu DT; Department of Chemistry and Bioengineering, University of Washington Seattle Washington 98195 USA chiu@uw.edu.

Publikováno v: RSC advances [RSC Adv] 2023 May 22; Vol. 13 (22), pp. 15121-15125. Date of Electronic Publication: 2023 May 22 (Print Publication: 2023).

High-Precision Mapping of Membrane Proteins on Synaptic Vesicles using Spectrally Encoded Super-Resolution Imaging.

Autor: Jiang Y; Institute of Basic Medicine and Cancer, Chinese Academy of Science, Hangzhou, Zhejiang 310016, China.; Departments of Chemistry and Bioengineering, University of Washington, Seattle, WA 98195, USA., Zhang J; Departments of Chemistry and Bioengineering, University of Washington, Seattle, WA 98195, USA., Jung SR; Departments of Chemistry and Bioengineering, University of Washington, Seattle, WA 98195, USA., Chen H; Departments of Chemistry and Bioengineering, University of Washington, Seattle, WA 98195, USA., Xu S; Departments of Chemistry and Bioengineering, University of Washington, Seattle, WA 98195, USA., Chiu DT; Departments of Chemistry and Bioengineering, University of Washington, Seattle, WA 98195, USA.

Publikováno v: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2023 Feb 13; Vol. 62 (8), pp. e202217889. Date of Electronic Publication: 2023 Jan 18.