Výsledky vyhledávání

Akademický článek

Construction of Dmd Gene Mutant Mice and Phenotype Verification in Muscle and Immune Systems

Autor: LIANG Min, GUO Yang, WANG Jinjin, ZHU Mengyan, CHI Jun, CHEN Yanjuan, WANG Chengji, YU Zhilan, SHEN Ruling

Publikováno v: Shiyan dongwu yu bijiao yixue, Vol 44, Iss 1, Pp 42-51 (2024)

Objective The aim is to utilize CRISPR/Cas9 gene editing technology to construct Dmd gene mutant mice with a point mutation in exon 23 of the Dmd gene. Subsequently, the phenotypic changes of the mice in muscles and immune systems are analyzed and ve

Externí odkaz: https://doaj.org/article/1152b6457674413fb72977efc60826ca

Zobrazit plný text záznamu

Akademický článek

Generation of 12 Drosophila Transgenic Negative Control Lines Based on Site-specific ΦC31 Integrase and pUASTattB Vector

Autor: XU Longmei, SHEN Ruling, FAN Chun, WU Wei

Publikováno v: Shiyan dongwu yu bijiao yixue, Vol 43, Iss 5, Pp 541-547 (2023)

Objective Construction of a negative control line for the Drosophila transgenic system based on ΦC31 integrase and vector plasmid pUASTattB to provide a more scientific negative control for transgenic Drosophila research experiments.Methods The vect

Externí odkaz: https://doaj.org/article/06769877bc5949dfad80eca3aa3e1b53

Zobrazit plný text záznamu

Akademický článek

Application of Optimized Latex Perfusion Technique in the Establishment of Craniofacial Venous Model in Mice

Autor: WANG Chengji, WANG Jue, WANG Haijie, LU Weisheng, SHI Yan, GU Zhengye, WAN Mingqiu, SHEN Ruling

Publikováno v: Shiyan dongwu yu bijiao yixue, Vol 43, Iss 5, Pp 574-578 (2023)

Objective Optimize the latex perfusion technique and apply it to the construction of a murine craniofacial venous vascular model. Methods A total of nine 8-week-old male C57BL/6 mice weighing (25.0±1.3) g were randomly divided into three groups: 60%

Externí odkaz: https://doaj.org/article/2839596aa60449c3a2e5d410189dc007

Zobrazit plný text záznamu

Akademický článek

Progress in Establishment and Application of Laboratory Animal Models Related to Development of Male Infertility Drugs

Autor: XIE Shuwu, SHEN Ruling, LIN Jinxing, FAN Chun

Publikováno v: Shiyan dongwu yu bijiao yixue, Vol 43, Iss 5, Pp 504-511 (2023)

As the incidence of male infertility has been increasing during recent years, it is urgent to reveal the pathogenesis of male infertility, as well as to develop the new drugs for treatment of male infertility, in order to solve the declining birth ra

Externí odkaz: https://doaj.org/article/4eb33b1da5cc4411add22b3e000814d7

Zobrazit plný text záznamu

Akademický článek

Progress in the Application of Animal Disease Models in the Medical Research on Colorectal Cancer

Autor: CHEN Yanjuan, SHEN Ruling

Publikováno v: Shiyan dongwu yu bijiao yixue, Vol 43, Iss 5, Pp 512-523 (2023)

Colorectal cancer (CRC) is the third most common malignant tumor in the world. The latest statistics show that CRC accounts for 10% of all cancer cases worldwide and is the second leading cause of cancer deaths. CRC is a highly heterogeneous disease,

Externí odkaz: https://doaj.org/article/bb93bbeb4a584cb78e9be737fbccddb3

Zobrazit plný text záznamu

Akademický článek

Anesthetic Effects of Different Doses of Zoletil Combined with Serazine Hydrochloride on C57BL/6J Mice

Autor: WANG Chengji, CHAI Qing, GONG Hui, WANG Jue, WAN Yinghan, GU Zhengye, BAO Xu, SHEN Ruling

Publikováno v: Shiyan dongwu yu bijiao yixue, Vol 42, Iss 1, Pp 31-35 (2022)

ObjectiveTo observe the duration of anesthesia in C57BL/6J mice anesthetized using different doses of Zoletil combined with serazine hydrochloride to provide an effective reference for all types of mouse surgery and related experimental design.Method

Externí odkaz: https://doaj.org/article/3217e345388649158637778a8650c00f

Zobrazit plný text záznamu