Electroporation of CRISPR/Cas9 Targeting Neurogenin 3 (NGN3) in Porcine Embryos and Its Effects on Mosaicism and Off-Target Effects by Next Generation Sequencing (NGS)

Autor: Insung Park, Sergio Navarro-Serna, Raquel M. Pinho, Trish Berger, Elizabeth A. Maga, Joaqu?n Gadea, Seung K. Kim, Pablo J. Ross

Publikováno v: Re:GEN Open, Vol 4, Iss 1, Pp 9-20 (2024)

Introduction: CRISPR/Cas9-mediated editing of embryos through microinjection is limited in efficiency because of high rates of mosaicism and off-target mutations. Electroporation has been proposed as an easier and faster procedure compared to single

Externí odkaz: https://doaj.org/article/ca79aef459f941d493f6c8f1535e9b85

Islet cell replacement and transplantation immunology in a mouse strain with inducible diabetes

Autor: Preksha Bhagchandani, Charles A. Chang, Weichen Zhao, Luiza Ghila, Pedro L. Herrera, Simona Chera, Seung K. Kim

Publikováno v: Scientific Reports, Vol 12, Iss 1, Pp 1-10 (2022)

Abstract Improved models of experimental diabetes are needed to develop cell therapies for diabetes. Here, we introduce the B6 RIP-DTR mouse, a model of experimental diabetes in fully immunocompetent animals. These inbred mice harbor the H2b major hi

Externí odkaz: https://doaj.org/article/9f14e82940fb47cfa087063f5419f40c

CRISPR-based genome editing in primary human pancreatic islet cells

Autor: Romina J. Bevacqua, Xiaoqing Dai, Jonathan Y. Lam, Xueying Gu, Mollie S. H. Friedlander, Krissie Tellez, Irene Miguel-Escalada, Silvia Bonàs-Guarch, Goutham Atla, Weichen Zhao, Seung Hyun Kim, Antonia A. Dominguez, Lei S. Qi, Jorge Ferrer, Patrick E. MacDonald, Seung K. Kim

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)

The editing of primary human islets could provide insight into diabetes pathogenesis. Here the authors use CRISPR-Cas9 to target regulatory elements associated with T2D susceptibility.

Externí odkaz: https://doaj.org/article/22d2b0339efc4aa29c30bb51db113c85

What is a β cell? – Chapter I in the Human Islet Research Network (HIRN) review series

Autor: Klaus H. Kaestner, Martha Campbell–Thompson, Yuval Dor, Ronald G. Gill, Benjamin Glaser, Seung K. Kim, Maike Sander, Cherie Stabler, Andrew F. Stewart, Alvin C. Powers

Publikováno v: Molecular Metabolism, Vol 53, Iss , Pp 101323- (2021)

Background: The pancreatic β cell, as the sole source of the vital hormone insulin, has been under intensive study for more than a century. Given the potential of newly created insulin-producing cells as a treatment or even cure of type 1 diabetes (

Externí odkaz: https://doaj.org/article/176e01f999a345899f3c242dc7786c64

An Interscholastic Network To Generate LexA Enhancer Trap Lines in Drosophila

Autor: Lutz Kockel, Catherine Griffin, Yaseen Ahmed, Lauren Fidelak, Arjun Rajan, Ethan P. Gould, Myles Haigney, Benjamin Ralston, Rex J. Tercek, Lara Galligani, Sagar Rao, Lutfi Huq, Hersh K. Bhargava, Ailis C. Dooner, Emily G. Lemmerman, Ruby F. Malusa, Tran H. Nguyen, Julie S. Chung, Sara M. Gregory, Kiyomasa M. Kuwana, Jonathan T. Regenold, Alexander Wei, Jake Ashton, Patrick Dickinson, Kate Martel, Connie Cai, Carissa Chen, Stephen Price, Jeffrey Qiao, David Shepley, Joanna Zhang, Meghana Chalasani, Khanh Nguyen, August Aalto, ByungJun Kim, Erik Tazawa-Goodchild, Amanda Sherwood, Ahmad Rahman, Sum Ying Celeste Wu, Joel Lotzkar, Serena Michaels, Hillary Aristotle, Antigone Clark, Grace Gasper, Evan Xiang, Frieda Luna Schlör, Melissa Lu, Kate Haering, Julia Friberg, Alyssa Kuwana, Jonathan Lee, Alan Liu, Emma Norton, Leena Hamad, Clara Lee, Dara Okeremi, Harry diTullio, Kat Dumoulin, Sun Yu Gordon Chi, Grayson S. Derossi, Rose E. Horowitch, Elias C. Issa, Dan T. Le, Bryce C. Morales, Ayush Noori, Justin Shao, Sophia Cho, Mai N. Hoang, Ian M. Johnson, Katherine C. Lee, Maria Lee, Elizabeth A. Madamidola, Katrina E. Schmitt, Gabriel Byan, Taeyoung Park, Jonathan Chen, Alexi Monovoukas, Madison J. Kang, Tanner McGowan, Joseph J. Walewski, Brennan Simon, Sophia J. Zu, Gregory P. Miller, Kate B. Fitzpatrick, Nicole Lantz, Elizabeth Fox, Jeanette Collette, Richard Kurtz, Chris Duncan, Ryan Palmer, Cheryl Rotondo, Eric Janicki, Townley Chisholm, Anne Rankin, Sangbin Park, Seung K. Kim

Publikováno v: G3: Genes, Genomes, Genetics, Vol 9, Iss 7, Pp 2097-2106 (2019)

Binary expression systems like the LexA-LexAop system provide a powerful experimental tool kit to study gene and tissue function in developmental biology, neurobiology, and physiology. However, the number of well-defined LexA enhancer trap insertions

Externí odkaz: https://doaj.org/article/fc21fc9a2ebf49d9b04f204517e30bf7