An improved bacterial single-cell RNA-seq reveals biofilm heterogeneity.

Autor: Yan X; The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Medical Research Institute, Wuhan University, Wuhan, China.; Frontier Science Center for Immunology and Metabolism, Wuhan University, Wuhan, China., Liao H; The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Medical Research Institute, Wuhan University, Wuhan, China.; Frontier Science Center for Immunology and Metabolism, Wuhan University, Wuhan, China.; Translational Medicine Research Center, North Sichuan Medical College, Nanchong, China., Wang C; The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Medical Research Institute, Wuhan University, Wuhan, China.; Frontier Science Center for Immunology and Metabolism, Wuhan University, Wuhan, China., Huang C; The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Medical Research Institute, Wuhan University, Wuhan, China.; Frontier Science Center for Immunology and Metabolism, Wuhan University, Wuhan, China., Zhang W; The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Medical Research Institute, Wuhan University, Wuhan, China.; Frontier Science Center for Immunology and Metabolism, Wuhan University, Wuhan, China., Guo C; Center for Life Sciences, School of Life Sciences, Yunnan University, Kunming, China., Pu Y; The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Medical Research Institute, Wuhan University, Wuhan, China.; Frontier Science Center for Immunology and Metabolism, Wuhan University, Wuhan, China.; Department of Immunology, Hubei Province Key Laboratory of Allergy and Immunology, State Key Laboratory of Virology and Medical Research Institute, Wuhan University School of Basic Medical Sciences, Wuhan, China.

Publikováno v: ELife [Elife] 2024 Dec 17; Vol. 13. Date of Electronic Publication: 2024 Dec 17.

Clustering-independent estimation of cell abundances in bulk tissues using single-cell RNA-seq data.

Autor: Aubin RG; Department of Genetics and Institute for Biomedical Informatics, Perelman School of Medicine, University of Pennsylvania, 3700 Hamilton Walk, Philadelphia, PA 19104, USA., Montelongo J; Department of Genetics and Institute for Biomedical Informatics, Perelman School of Medicine, University of Pennsylvania, 3700 Hamilton Walk, Philadelphia, PA 19104, USA., Hu R; Department of Genetics and Institute for Biomedical Informatics, Perelman School of Medicine, University of Pennsylvania, 3700 Hamilton Walk, Philadelphia, PA 19104, USA., Gunther E; Department of Genetics and Institute for Biomedical Informatics, Perelman School of Medicine, University of Pennsylvania, 3700 Hamilton Walk, Philadelphia, PA 19104, USA., Nicodemus P; Department of Genetics and Institute for Biomedical Informatics, Perelman School of Medicine, University of Pennsylvania, 3700 Hamilton Walk, Philadelphia, PA 19104, USA., Camara PG; Department of Genetics and Institute for Biomedical Informatics, Perelman School of Medicine, University of Pennsylvania, 3700 Hamilton Walk, Philadelphia, PA 19104, USA. Electronic address: pcamara@pennmedicine.upenn.edu.

Publikováno v: Cell reports methods [Cell Rep Methods] 2024 Nov 18; Vol. 4 (11), pp. 100905.

Identification and evaluation of plasma exosome RNA biomarkers for non-invasive diagnosis of hepatocellular carcinoma using RNA-seq.

Autor: Huang H; Infectious Disease Department, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China., Zhang M; BamRock Research Department, Suzhou BamRock Biotechnology Ltd., Suzhou, Jiangsu Province, China., Lu H; Infectious Disease Department, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China., Chen Y; Infectious Disease Department, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China., Sun W; Ulink College of Shanghai, Shanghai, China., Zhu J; Infectious Disease Department, The Fourth Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China. zhujinghan@139.com., Chen Z; Infectious Disease Department, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China. chenzt@suda.edu.cn.; MOE Key Laboratory of Geriatric Diseases and Immunology, Suzhou Key Laboratory of Pathogen Bioscience and Anti-infective Medicine, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China. chenzt@suda.edu.cn.; Infectious Disease Department, The Fourth Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China. chenzt@suda.edu.cn.

Publikováno v: BMC cancer [BMC Cancer] 2024 Dec 18; Vol. 24 (1), pp. 1552. Date of Electronic Publication: 2024 Dec 18.

Single-cell RNA-seq reveals FGF12 as a prognostic biomarker in low-grade endometrial stromal sarcoma.

Autor: Miao Y; Department of Oncology, The Second Affiliated Hospital of Zunyi Medical University, Zunyi, China.; Institute of Biomedical Research, Henan Academy of Sciences, Zhengzhou, China., Dong M; Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology and University of Tübingen, Stuttgart, Germany., Zhou Q; Department of Obstetrics and Gynecology, Yanhe County People's Hospital, Tongren, Guizhou, China., Thiel J; Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology and University of Tübingen, Stuttgart, Germany., Li N; Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China., Cai Y; Department of Oncology, The Second Affiliated Hospital of Zunyi Medical University, Zunyi, China., Yuan D; Department of Pathology, The Affiliated Hospital of Zunyi Medical University, Zunyi, China., Wang H; Thoracic Surgery Branch, Center for Cancer Research, National Cancer Institute (NCI), NIH, Bethesda, MD, United States., Jin SH; Department of Orthodontics, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi, China., Yang H; Department of Pathology, The Second Affiliated Hospital of Zunyi Medical University, Zunyi, China., Wang J; Department of Pathology, The Affiliated Hospital of Zunyi Medical University, Zunyi, China., Frey B; Translational Radiobiology, Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.; Comprehensive Cancer Center Erlangen, European Metropolitan Region Nuremberg, Erlangen, Germany.; FAU Profile Center for Immunomedicine, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany., Gaipl US; Translational Radiobiology, Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.; Comprehensive Cancer Center Erlangen, European Metropolitan Region Nuremberg, Erlangen, Germany.; FAU Profile Center for Immunomedicine, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany., Ma H; Department of Oncology, The Second Affiliated Hospital of Zunyi Medical University, Zunyi, China., Zhou JG; Department of Oncology, The Second Affiliated Hospital of Zunyi Medical University, Zunyi, China.; Translational Radiobiology, Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.; Comprehensive Cancer Center Erlangen, European Metropolitan Region Nuremberg, Erlangen, Germany.; FAU Profile Center for Immunomedicine, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.

Publikováno v: Frontiers in immunology [Front Immunol] 2024 Nov 29; Vol. 15, pp. 1513076. Date of Electronic Publication: 2024 Nov 29 (Print Publication: 2024).

Cross-species single-cell RNA sequencing reveals divergent phenotypes and activation states of adaptive immunity in human carotid and experimental murine atherosclerosis.

Autor: Horstmann H; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.; Division of Cardiology, Department of Medicine, NYU Cardiovascular Research Center, NYU Grossmann School of Medicine, 10016 New York, NY, USA., Michel NA; Department of Cardiology, University Heart Center Graz, Medical University of Graz, 8036 Graz, Austria., Sheng X; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany., Hansen S; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany., Lindau A; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany., Pfeil K; Division of Cardiology, Department of Medicine, NYU Cardiovascular Research Center, NYU Grossmann School of Medicine, 10016 New York, NY, USA., Fernández MC; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.; Institute for Experimental Cardiovascular Medicine, Heart Centre, University of Freiburg, 79106 Freiburg, Germany., Marchini T; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany., Winkels H; Department of Cardiology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50923 Cologne, Germany., Mitre LS; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, 79106 Freiburg, Germany., Abogunloko T; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, 79106 Freiburg, Germany., Li X; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany., Mwinyella TB; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany., Gissler MC; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany., Bugger H; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.; Department of Cardiology, University Heart Center Graz, Medical University of Graz, 8036 Graz, Austria., Heidt T; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany., Buscher K; Division of General Internal Medicine, Nephrology and Rheumatology, Department of Medicine, University Hospital of Münster, 48149 Münster, Germany., Hilgendorf I; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany., Stachon P; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany., Piepenburg S; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany., Verheyen N; Department of Cardiology, University Heart Center Graz, Medical University of Graz, 8036 Graz, Austria., Rathner T; Department of Cardiology, University Heart Center Graz, Medical University of Graz, 8036 Graz, Austria., Gerhardt T; Department of Cardiology, Angiology and Intensive Care Medicine CBF, Deutsches Herzzentrum der Charité, and Berlin Institute of Health (BIH), 13353 Berlin, Germany.; DZHK (German Centre for Cardiovascular Research), partner site Berlin , 13353 Berlin, Germany.; Cardiovascular Research Institute and the Department of Medicine, Cardiology, Icahn School of Medicine at Mount Sinai, 10029 New York, NY, USA., Siegel PM; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany., Oswald WK; Department of Vascular Surgery, Medical University of Graz, 8036 Graz, Austria., Cohnert T; Department of Vascular Surgery, Medical University of Graz, 8036 Graz, Austria., Zernecke A; Institute of Experimental Biomedicine, University Hospital Würzburg, 97080 Würzburg, Germany., Madl J; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.; Institute for Experimental Cardiovascular Medicine, Heart Centre, University of Freiburg, 79106 Freiburg, Germany., Kohl P; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.; Institute for Experimental Cardiovascular Medicine, Heart Centre, University of Freiburg, 79106 Freiburg, Germany., Foks AC; Leiden Academic Centre for Drug Research, Division of BioTherapeutics, Leiden University, 2333 CC Leiden, The Netherlands., von Zur Muehlen C; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany., Westermann D; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany., Zirlik A; Department of Cardiology, University Heart Center Graz, Medical University of Graz, 8036 Graz, Austria., Wolf D; Department of Cardiology and Angiology I, Medical Center, University of Freiburg, 79106 Freiburg, Germany.; Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.

Publikováno v: Cardiovascular research [Cardiovasc Res] 2024 Nov 25; Vol. 120 (14), pp. 1713-1726.