Accurate serology for SARS-CoV-2 and common human coronaviruses using a multiplex approach

Autor: van Tol, Sophie, Mögling, Ramona, Li, Wentao, Godeke, Gert-Jan, Swart, Arno, Bergmans, Barbara, Brandenburg, Afke, Kremer, Kristin, Murk, Jean-Luc, van Beek, Josine, Wintermans, Bas, Reimerink, Johan, Bosch, Berend-Jan, Reusken, Chantal, Virologie, dI&I I&I-1

Publikováno v: Emerging Microbes and Infections, 9(1), 1965. Nature Publishing Group
Emerging Microbes & Infections
article-version (VoR) Version of Record

Serology is a crucial part of the public health response to the ongoing SARS-CoV-2 pandemic. Here, we describe the development, validation and clinical evaluation of a protein micro-array as a quantitative multiplex immunoassay that can identify S an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce7975ae14df1afcc8ef5b9bef2c2c0f
https://doi.org/10.1080/22221751.2020.1813636

Antigenicity and immunogenicity of SARS-CoV S protein receptor-binding domain stably expressed in CHO cells

Autor: Lin Li, Lanying Du, Yuxian He, Guangyu Zhao, Yusen Zhou, Shibo Jiang, Bo-Jian Zheng

Publikováno v: Biochemical and Biophysical Research Communications

The receptor-binding domain (RBD) of SARS coronavirus (SARS-CoV) spike (S) protein contains multiple conformation-dependent epitopes that induce neutralizing antibody responses. Here we used CHO-K1 cells to establish a cell line for stable expression

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17e356f9c2ac96a0d29964595332c2a5

Sensitive and Specific Monoclonal Antibody-Based Capture Enzyme Immunoassay for Detection of Nucleocapsid Antigen in Sera from Patients with Severe Acute Respiratory Syndrome

Autor: Xu Hua, Ya-Di Wang, Xiao-Yan Che, Li Wen Qiu, Kun Wen, Yu Xian Pan, Kwok-Yung Yuen, Li ya Zhang, Wei Hao, Zhi Yong Liao, Vincent C.C. Cheng

Publikováno v: Journal of Clinical Microbiology

A rapid antigen test for the diagnosis of severe acute respiratory syndrome (SARS) is essential for control of this disease at the point of management. The nucleocapsid (N) protein of SARS-associated coronavirus (SARS-CoV) is abundantly expressed in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2846d0861a24a7659a23e5530437049f
https://doi.org/10.1128/jcm.42.6.2629-2635.2004

Toll-like receptors, chemokine receptors and death receptor ligands responses in SARS coronavirus infected human monocyte derived dendritic cells

Autor: Yu-Lung Lau, Helen K. W. Law, Sin Fun Sia, Yuk On Chan, J. S. Malik Peiris, Chung Yan Cheung

Publikováno v: BMC Immunology
BMC Immunology, Vol 10, Iss 1, p 35 (2009)
BMC immunology, 10(1):35

Background: The SARS outbreak in 2003 provides a unique opportunity for the study of human responses to a novel virus. We have previously reported that dendritic cells (DCs) might be involved in the immune escape mechanisms for SARS-CoV. In this stud

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3640138a402506174c18aae590dd38f7
https://doi.org/10.1186/1471-2172-10-35

False-Positive Results in a Recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) Nucleocapsid Enzyme-Linked Immunosorbent Assay Due to HCoV-OC43 and HCoV-229E Rectified by Western Blotting with Recombinant SARS-CoV Spike Polypeptide

Autor: Wai-ting Hui, Robert B. Couch, Kwok-Hung Chan, Susanna K. P. Lau, Patrick C. Y. Woo, Jsm Peiris, Beatrice H.L. Wong, Grace See-Wai Kwan, Kwok-Yung Yuen

Publikováno v: Journal of Clinical Microbiology

Using paired serum samples obtained from patients with illness associated with increases in anti-human coronavirus OC43 (HCoV-OC43) or anti-HCoV-229E antibodies, we examined the possibility of false-positive results detected in a recombinant severe a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6104254c60e2960c5258081307e00417
https://europepmc.org/articles/PMC535232/