Zobrazeno 1 - 10
of 12
pro vyhledávání: '"S.Ronald Smithers"'
Autor:
Andrew J.G. Simpson, Rodrigues Correa-Oliveira, Raymond Allen, S.Ronald Smithers, Paul Hagan, Simon A. Jeffs
Publikováno v:
Molecular and biochemical parasitology. 46(1)
A cDNA clone from an adult Schistosoma mansoni λgt11 expression library (A12) encoding an antigenic polypeptide of 22 kDa is described. A12 is 797 bp long and has one open reading frame encoding a protein of 190 amino acids which does not contain a
Autor:
Andrew J.G. Simpson, S.Ronald Smithers, Trudi Hollyer, Frederico G.C. Abath, Pirlanta Omer Ali, Raymond Allen, Simon A. Jeffs, Carolyn A. Owen, Helen M. Meadows, Fiona Hackett
Publikováno v:
Molecular and biochemical parasitology. 45(2)
Sm25 is the principal antigen recognised by antibodies from mice protectively vaccinated with isolated tegumental membranes of adult Schistosoma mansoni. The full-length amino acid sequence of this protein has been deduced from the sequence of two cD
Autor:
J.E. Lillywhite, Paul Hagan, Xinyuan Yi, Franklin David Rumjanek, Rodrigo Corrêa Oliveira, Naftale Katz, Richard Mott, Pirlanta Omer Ali, Andrew J.G. Simpson, C. Kelly, S.Ronald Smithers
Publikováno v:
Transactions of the Royal Society of Tropical Medicine and Hygiene. 84(6)
Immunoglobulin (Ig) G and IgM antibody levels to soluble egg antigens (SEA), adult worm glycoproteins (AWGP), carbohydrate antigens (CHO) and cationic exchange fraction 6 (CEF6) were measured in serum specimens taken from Brazilian patients with acut
Publikováno v:
Molecular and Biochemical Parasitology. 17:115-130
The structures of the 38, 32 and 20 kDa surface antigens isolated from schistosomula and adult worms of Schistosoma mansoni were compared by two-dimensional peptide mapping, by immunological analysis and by one- and two-dimensional electrophoresis. P
Publikováno v:
Molecular and Biochemical Parasitology. 8:191-205
Lectins labeled with 125I or conjugated with fluorescein were employed to study the carbohydrates on the surface of different stages of schistosomula of Schistosoma mansoni. Newly transformed schistosomula were shown to bind concanavalin A; the 60 00
Publikováno v:
Experimental Parasitology. 60:304-308
Two-dimensional gel analysis of the surface polypeptides of the schistosomula stage of Schistosoma mansoni resolved a complex pattern of approximately 20 polypeptides. The majority of these were identified as immunogenic since they were immunoprecipi
Autor:
Quentin D. Bickle, S.Ronald Smithers, Andrew J.G. Simpson, Andrew Wilkins, Matty Knight, Paul Hagan, Ann Moloney
Publikováno v:
Molecular and Biochemical Parasitology. 18:235-253
Protective immunity has been demonstrated in experimental schistosomiasis and is also believed to occur in man. It can be mediated by antibodies from infected animals or animals immunized with attenuated organisms. Recombinant Escherichia coli synthe
Publikováno v:
Experimental Parasitology. 41:160-166
Sera obtained from mice 12–16 weeks after a single infection of Schistosoma mansoni were separated by gel filtration or DEAE-cellulose chromatography and the fractions were tested for their activity in passively immunizing normal recipients. Partia
Publikováno v:
Molecular and Biochemical Parasitology. 9:337-350
When freshly transformed schistosomula of Schistosoma mansoni are incubated with human serum, a protein doublet (molecular weight approximately 45 kDa) is expressed on the surfaces of the parasites; parasites incubated in defined media fail to expres
Autor:
S.Ronald Smithers, Franklin David Rumjanek, Maliha Chaudri, Andrew J. G. Simpson, Matty Knight, Charles Kelly, Stella Martin
Publikováno v:
Molecular and Biochemical Parasitology. 22:169-176
A previously described cDNA clone, pSF10, of Schistosoma mansoni encoding the very dominant female specific polypeptide (FSP) has been used to characterize the gene and its expression. The gene is detectable in different isolates of S. mansoni and is