Zobrazeno 1 - 10
of 266
pro vyhledávání: '"S.P. Leibo"'
Autor:
S.P. Leibo, Kenneth L. Jones, Jerome J. Howard, Scott G. Hereford, Jessica R. Henkel, Megan L. Savoie
Publikováno v:
Zoo Biology. 31:322-335
The minimization of kinship in captive populations is usually achieved through the use of pedigree information. However, pedigree knowledge alone is not sufficient if pedigree information is missing, questionable, or when the founders of the captive
Publikováno v:
Cell Preservation Technology. 4:188-198
The osmotic responses of isolated ovine primordial follicles when suspended in various concentrations of several cryoprotective additives (CPAs) have recently been published. We have used the Kedem-Katchalsky formulation, written with two parameters
Publikováno v:
Molecular Reproduction and Development. 50:485-492
Activation of bovine oocytes to produce a single haploid pronucleus in preparation for intracytoplasmic sperm injection (ICSI) has been investigated with various combinations of ionomycin and 6-dimethylaminopurine (DMAP). Effects were evaluated by im
Publikováno v:
The Journal of Experimental Zoology. 280:189-196
The objective of this study was to determine the viability and fertility of mouse spermatozoa obtained at various postmortem intervals. Male mice were euthanized, and the bodies were kept at approximately 22 degrees C for up to 24 hr. The epididymide
Publikováno v:
Theriogenology. 48:1329-1342
Successful cryopreservation of spermatozoa for genome banking or transport for articifial insemination (AI) in domestic and nondomestic Canidae depends on understanding the species-specific conditions required. Experiment 1 was designed to evaluate t
Autor:
S.P. Leibo, Nucharin Songsasen
Publikováno v:
Cryobiology. 35:255-269
To examine the effect of seeding to induce ice formation during cryopreservation on their survival, spermatozoa from B6D2F1 mice were cooled to and held at −4°C for 30 min in phosphate-buffered saline (PBS) alone, in egg yolk-supplemented PBS, or
Publikováno v:
Biology of Reproduction. 56:143-152
The objective of this study was to develop a reliable method to cryopreserve mouse spermatozoa, using in vitro fertilization (IVF) of oocytes, development of resultant zygotes into blastocysts, and the birth of live young after embryo transfer as cri
Publikováno v:
Molecular Reproduction and Development. 45:503-512
Bovine oocytes are damaged when chilled to temperatures near 0 degree C. We have determined the temperatures at which this injury occurs, as well as its kinetics and the functional consequences for oocytes both at the germinal vesicle-stage (GV) and
Publikováno v:
Theriogenology. 46:849-858
Recovery of oocytes from ovaries collected at slaughter was carried out at three ambient temperatures (25 degrees, 30 degrees and 35 degrees C) to assess the effect on subsequent embryonic production in vitro. Oocytes recovered at each temperature we