Výsledky vyhledávání - "S. K. Skelton"

Chlamydia DNA extraction for use in PCR: Stability and sensitivity in detection

Autor: Trudy O. Messmer, Harry Daugharty, S K Skelton

Publikováno v: Journal of Clinical Laboratory Analysis. 12:47-53

We evaluated multiple procedures for extracting chlamydial DNA from specimens for detection in PCR tests. Commercial kits and an in‐house method were tested for their sensitivity and utility. Quantifiable chlamydial elementary bodies (EB) were used

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45254b5624da75cb9940874020e1dcb6
https://doi.org/10.1002/(sici)1098-2825(1998)12:1<47::aid-jcla8>3.0.co

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Utility of complement fixation and microimmunofluorescence assays for detecting serologic responses in patients with clinically diagnosed psittacosis

Autor: K H Wong, Harry Daugharty, S K Skelton

Publikováno v: Journal of Clinical Microbiology. 32:2417-2421

The serodiagnosis of human psittacosis was considerably improved by a microimmunofluorescence (MIF) assay that uses selected strains of Chlamydia psittaci, C. pneumoniae, and C. trachomatis as antigens. The 78 patients examined in the study were clin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a331d43328ee4896c9f69ba3781dd53
https://doi.org/10.1128/jcm.32.10.2417-2421.1994

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Radiographic appearance of Chlamydia pneumoniae (TWAR strain) respiratory infections. CBPIS Study Group. Community-based Pneumonia Incidence Study

Autor: C. F. Mueller, Kwei-Hay Wong, J. F. Plouffe, R. F. Breiman, B. J. Marston, C. T. Mcconnell, T. M. File, S. K. Skelton

Publikováno v: Radiology. 192:819-824

PURPOSE: To report the spectrum of radiographic findings associated with a new respiratory pathogen: Chlamydia pneumoniae (TWAR strain). MATERIALS AND METHODS: Radiographs of 55 adult patients hospitalized with serologic evidence of C pneumoniae were

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fbe2914eee6320b2498e3b144d98b242
https://doi.org/10.1148/radiology.192.3.8058954

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Efficient culture of Chlamydia pneumoniae with cell lines derived from the human respiratory tract

Autor: K H Wong, S K Skelton, Y. K. Chan

Publikováno v: Journal of Clinical Microbiology. 30:1625-1630

Two established cell lines, H 292 and HEp-2, originating from the human respiratory tract, were found to be significantly more efficient and practical than the currently used HeLa 229 cells for growth of Chlamydia pneumoniae. Six strains of C. pneumo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4116261de6a2c3f6a7b99c8f4ef05c41
https://doi.org/10.1128/jcm.30.7.1625-1630.1992

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Application of a nested, multiplex PCR to psittacosis outbreaks

Autor: Harry Daugharty, Trudy O. Messmer, Barry S. Fields, S K Skelton, John F. Moroney

Publikováno v: Journal of clinical microbiology. 35(8)

We developed a nested, multiplex PCR for simultaneous detection of three species of chlamydiae in human and avian specimens. The PCR was designed to increase sensitivity and to circumvent inhibitors of PCR present in clinical specimens. The target se

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91e02f78030615cdbb63bc96d31e693e
https://pubmed.ncbi.nlm.nih.gov/9230378

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Simple, efficient purification of filamentous hemagglutinin and pertussis toxin from Bordetella pertussis by hydrophobic and affinity interaction

Autor: S K Skelton, K H Wong

Two major antigens of Bordetella pertussis, filamentous hemagglutinin (FHA) and pertussis toxin (PT), were efficiently purified from culture filtrate by exploiting their relative hydrophobicities and differences in affinity to sialic acid-containing

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b431618300f329cf153639a4427031c
https://europepmc.org/articles/PMC267867/

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New, practical approach to detecting antibody to pertussis toxin for public health and clinical laboratories

Autor: K H Wong, S K Skelton

Publikováno v: Journal of Clinical Microbiology. 26:1316-1320

A new, practical method for determining antibody to pertussis toxin (PT) by enzyme-linked immunosorbent assay for public health and clinical laboratories is possible because of recent advances in understanding the pathobiology of Bordetella pertussis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::816ef183d7005d9da79bdf54af39107e
https://doi.org/10.1128/jcm.26.7.1316-1320.1988

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Typing of heat-stable and heat-labile antigens of Campylobacter jejuni and Campylobacter coli by coagglutination

Autor: K H Wong, C M Patton, James C. Feeley, George K. Morris, S K Skelton

A coagglutination system has been devised for typing heat-stable and heat-labile antigens of Campylobacter jejuni and C. coli. The use of protein A-positive Staphylococcus aureus cells carrying Campylobacter sp. serotype antibody and the treatment of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d710e2bc2319757018dc951e780c751c
https://europepmc.org/articles/PMC271762/

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Strain characterization and grouping of Campylobacter jejuni and Campylobacter coli by interaction with lectins

Autor: J C Feeley, S K Skelton, K H Wong

Strains of Campylobacter jejuni and Campylobacter coli were characterized and grouped by their distinct reaction patterns with lectins. Heating of the Campylobacter cultures to 100 degrees C and holding for 30 to 60 min greatly enhanced their reactiv

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c22f2aab40d8e207484d7cdbbb19a7ab
https://europepmc.org/articles/PMC268663/

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