Zobrazeno 1 - 10
of 16
pro vyhledávání: '"S. H. Bissbort"'
Autor:
Megan Jean Bester, John Elias, William James Hayward Vermaak, Gurdeep S. Dhatt, Joachim K.W. Pum, S. H. Bissbort
Publikováno v:
Clinica Chimica Acta. 303:139-145
Background: Because of the lack of a problem-free, reliable method for determination of erythrocyte acetylcholinesterase (AChE), we developed a simple kinetic method, which we found to be both reliable and suitable for automation in the routine clini
Publikováno v:
Clinica Chimica Acta. 223:148-157
We have developed a new enzymatic assay for the determination of inorganic phosphate (Pi) in serum, using nucleoside phosphorylase (NP) and xanthine oxidase (XOD). Pi and inosine react to form hypoxanthine and ribose-1-phosphate. The hypoxanthine is
Publikováno v:
Clinical Chemistry. 39:2182-2185
A new kinetic method for the determination of serum adenosine deaminase (EC 3.5.4.4) is described, with adenosine as the substrate and nucleoside phosphorylase and xanthine oxidase as the reaction enzymes. Inosine is produced, which is converted to h
Publikováno v:
Clinical Chemistry. 38:1322-1326
Human adenosine deaminase (ADA; EC 3.5.4.4) consists of three isoenzymes: ADA1, ADA1+CP, and ADA2. We developed an electrophoretic technique to distinguish between these three isoenzymes. The isoenzyme pattern was studied in tissue and cell homogenat
Autor:
Johan B. Ubbink, W. J. H. Vermaak, S. H. Bissbort, Rhena Delport, Piet J. Becker, Jacobus P.J. Ungerer
Publikováno v:
Atherosclerosis. 89:155-162
Dietary fat intake is often regarded as a major determinant of coronary heart disease (CHD) rate and it has been deemed unnecessary to invoke racial or other factors to explain the differences in CHD rates among different ethnic groups. Despite a hig
Autor:
Johan B. Ubbink, W. J. H. Vermaak, Piet J. Becker, D. A. van Staden, J. M. Bennett, S. H. Bissbort
Publikováno v:
Klinische Wochenschrift. 69:527-534
Total serum homocysteine and cholesterol levels were determined in 163 male patients with typical angina who were subjected to coronary angiography. The prevalence of homocysteinemia in coronary heart disease (CHD) was 41.9%. Serum homocysteine level
Publikováno v:
Journal of Chromatography B: Biomedical Sciences and Applications. 566:369-375
Human lymphocyte kynureninase activity was assessed in homogenized cells by determination of 3-hydroxyanthranilic acid production as a function of time after addition of the substrate, 3-hydroxykynurenine. The product, 3-hydroxyanthranilic acid, was
Publikováno v:
Journal of Chromatography B: Biomedical Sciences and Applications. 565:441-446
A rapid, isocratic high-performance liquid chromatographic (HPLC) method is described for the determination of total homocysteine levels in human serum. Prior to reversed-phase HPLC analysis, the serum thiols were derivatized with SBD-F (ammonium 7-f
Publikováno v:
European Journal of Clinical Microbiology & Infectious Diseases. 15:510-512
The activity of serum adenosine deaminase and its isoenzymes (ADA1 and ADA2) was measured in the sera of 55 patients with clinically diagnosed typhoid fever, 46 of whom were seropositive for Salmonella typhi infection. At presentation 95% of the sero
Autor:
Megan Jean Bester, W. J. H. Vermaak, Hendrik C. Potgieter, S. H. Bissbort, J. H. Spies, Johan B. Ubbink
Publikováno v:
Analytical biochemistry. 248(1)
Plasma methionine (Met), methionine sulfoxide (MSO), and total Met concentrations were determined by reversed-phase chromatography and fluorescence detection after automated precolumn derivatization with an o-phthalic aldehyde mercaptoethanol reagent