Zobrazeno 1 - 10 of 21 pro vyhledávání: '"S. E. Halford"'
1
The identification of chemical intermediates in enzyme catalysis by the rapid quench-flow technique
Autor: H. Gutfreund, Tom Barman, S. E. Halford, Corinne Lionne, S. R. W. Bellamy
Publikováno v: Cellular and molecular life sciences : CMLS. 63(22)
Traditionally, enzyme transient kinetics have been studied by the stopped-flow and rapid quench-flow (QF) methods. Whereas stopped-flow is the more convenient, it suffers from two weaknesses: optically silent systems cannot be studied, and when there
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dfec0591a2d5565c74e5924eab562e62
https://pubmed.ncbi.nlm.nih.gov/16952048
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2
DNA cleavage reactions by type II restriction enzymes that require two copies of their recognition sites
Autor: M L, Embleton, V, Siksnys, S E, Halford
Publikováno v: Journal of molecular biology. 311(3)
Several type II restriction endonucleases interact with two copies of their target sequence before they cleave DNA. Three such enzymes, NgoMIV, Cfr10I and NaeI, were tested on plasmids with one or two copies of their recognition sites, and on catenan
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::b1cec79100c1dc0d9b10bc896d3d6f08
https://pubmed.ncbi.nlm.nih.gov/11493004
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3
Analysis of DNA looping interactions by type II restriction enzymes that require two copies of their recognition sites
Autor: S E, Milsom, S E, Halford, M L, Embleton, M D, Szczelkun
Publikováno v: Journal of molecular biology. 311(3)
Before cleaving DNA substrates with two recognition sites, the Cfr10I, NgoMIV, NaeI and SfiI restriction endonucleases bridge the two sites through 3D space, looping out the intervening DNA. To characterise their looping interactions, the enzymes wer
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6849f1588d5be5a2ac057c627428ae35
https://pubmed.ncbi.nlm.nih.gov/11493005
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5
Phosphorothioate substrates for the SfiI restriction endonuclease
Autor: T J, Nobbs, S A, Williams, B A, Connolly, S E, Halford
Publikováno v: Biological chemistry. 379(4-5)
Oligodeoxynucleotides carrying the recognition sequence for the SfiI endonuclease were synthesised with phosphorothioates at the cleavage site. The Rp and Sp diastereoisomers of the oligonucleotides were separated by HPLC using a mobile phase contain
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e7519ff149a1a87fcde2d8be502c5ce7
https://pubmed.ncbi.nlm.nih.gov/9628364
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6
Repercussions of DNA tracking by the type IC restriction endonuclease EcoR124I on linear, circular and catenated substrates
Autor: M D, Szczelkun, M S, Dillingham, P, Janscak, K, Firman, S E, Halford
Publikováno v: The EMBO journal. 15(22)
Type I restriction endonucleases such as EcoR124I cleave DNA at undefined loci, distant from their recognition sequences, by a mechanism that involves the enzyme tracking along the DNA between recognition and cleavage sites. This mechanism was examin
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::cb83317b4321388b9ebe361e6b7c29a4
https://pubmed.ncbi.nlm.nih.gov/8947056
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10
Single turnovers of the EcoRI restriction endonuclease
Autor: N P Johnson, S E Halford
Publikováno v: Biochemical Journal. 211:405-415
Single turnovers of the EcoRI restriction endonuclease, cleaving its recognition site on the covalently closed form of plasmid pMB9, were examined. Two methods were used to monitor the progress of the reactions: one involved quenching the reaction at
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5fb7c3797a98858787db4a29a3d71a7
https://doi.org/10.1042/bj2110405
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